Dynamic of the structural alteration of biochar in ancient Anthrosol over a long timescale by Raman spectroscopy

Sousa, Daniel Vieira de; Guimarães, Luciano Moura; Felix, Jorlandio F.; Ker, João Carlos; Schaefer, Carlos E. G. R.; Rodet, Maria Jacqueline

doi:10.1371/journal.pone.0229447

Cited by 29 publications

(16 citation statements)

References 60 publications

(94 reference statements)

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“…2b ) of CKMB and laccase@CKMB samples do not show any significant differences, indicates that the immobilization process of laccase did not affect the structure of CKMB. In addition, Biochar has a disordered structure of amorphous phase, which is mainly caused by the uneven pyrolysis of molecules in the pyrolysis process of biochar [ 22 ]. In short, the results of XRD and FTIR help to some extent prove that laccase is well loaded on CKMB.…”

Section: Resultsmentioning

confidence: 99%

The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar

et al. 2021

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Background Although laccase has a good catalytic oxidation ability, free laccase shows a poor stability. Enzyme immobilization is a common method to improve enzyme stability and endow the enzyme with reusability. Adsorption is the simplest and common method. Modified biochar has attracted great attention due to its excellent performance. Results In this paper, cetyltrimethylammonium bromide (CTAB)-KOH modified biochar (CKMB) was used to immobilize laccase by adsorption method (laccase@CKMB). Based on the results of the single-factor experiments, the optimal loading conditions of laccase@CKMB were studied with the assistance of Design-Expert 12 and response surface methods. The predicted optimal experimental conditions were laccase dosage 1.78 mg/mL, pH 3.1 and 312 K. Under these conditions, the activity recovery of laccase@CKMB was the highest, reaching 61.78%. Then, the CKMB and laccase@CKMB were characterized by TGA, FT-IR, XRD, BET and SEM, and the results showed that laccase could be well immobilized on CKMB, the maximum enzyme loading could reach 57.5 mg/g. Compared to free laccase, the storage and pH stability of laccase@CKMB was improved greatly. The laccase@CKMB retained about 40% of relative activity (4 °C, 30 days) and more than 50% of relative activity at pH 2.0–6.0. In addition, the laccase@CKMB indicated the reusability up to 6 reaction cycles while retaining 45.1% of relative activity. Moreover, the thermal deactivation kinetic studies of laccase@CKMB showed a lower k value (0.00275 min− 1) and higher t1/2 values (252.0 min) than the k value (0.00573 min− 1) and t1/2 values (121.0 min) of free laccase. Conclusions We explored scientific and reasonable immobilization conditions of laccase@CKMB, and the laccase@CKMB possessed relatively better stabilities, which gave the immobilization of laccase on this cheap and easily available carrier material the possibility of industrial applications.

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Section: Resultsmentioning

confidence: 99%

The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar

et al. 2021

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“…The G band noticed nearly 1580 cm −1 in graphite material which is an indication of the presence of organised sp2 domains. The D band noticed at approximately 1350 cm −1 and is related to the defects in the graphene sheet (Sousa et al, 2020).…”

Section: Analysis Of Corn Cob-derived Biocharmentioning

confidence: 99%

A holistic overview on corn cob biochar: A mini-review

Wakudkar

Jain

2022

Waste Manag Res

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Corn cob is one of the agricultural waste materials subjected to improper burning, which creates pollution. It can be used for the production of green technologies for further applications. Carbonisation or slow pyrolysis could be promising alternative to burning. It has many applications, such as soil ameliorant, waste water treatment, carbon sequestration, composting, supercapacitor, fuel cell and biocomposites material. It motivated to investigate the suitability of corn cob as a potential material for biochar production and its application. The advanced form of analysis, such as thermogravimetric, scanning electron microscopy, surface area, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy and Raman spectroscopy, is elaborated for in-depth knowledge of characteristics. The hypothesis is that if the available corn cob is used for biochar production, it will reduce the carbon dioxide (CO2) emission. On a global level, conversion of available corn cob into biochar is expected to reduce CO2 emission by 0.13 Gt per year. The reduction in CO2 emission also favours economy. If 1 tonne of biomass per year is converted into biochar, 0.82 tonnes of CO2 can be reduced per year and by considering the emission cost of Rs 1800 per tonne, the cost saving would be Rs 1476 per year. The presented mini-review article provides an outline of the state-of-art information on corn cob biochar and its novel application. It will be helpful to scientific domain to find new opportunities in biochar research and also the humanity will be benefitted due to reduction in greenhouse gases.

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“…2b) of CKMB and laccase@CKMB samples do not show any significant differences, indicates that the immobilization process of laccase did not affect the structure of CKMB. In addition, Biochar has a disordered structure of amorphous phase, which is mainly caused by the uneven pyrolysis of molecules in the pyrolysis process of biochar [22].…”

Section: Characterization Analysismentioning

confidence: 99%

The Study of Laccase Immobilization Optimization and Stability Improvement on CTAB-KOH Modified Biochar

Wang

Ren

Jiang

et al. 2021

Preprint

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Background: Although laccase has a good catalytic oxidation ability, free laccase shows a poor stability. Enzyme immobilization is a common method to improve enzyme stability and endow the enzyme with reusability. Adsorption is the simplest and common method. Modified biochar has attracted great attention due to its excellent performance. Results: In this paper, cetyltrimethylammonium bromide (CTAB)-KOH modified biochar (CKMB) was used to immobilize laccase by adsorption method (laccase@CKMB). Based on the results of the single-factor experiments, the optimal loading conditions of laccase@CKMB were studied with the assistance of Design-Expert 12 and response surface methods. The predicted optimal experimental conditions were laccase dosage 1.78 mg/mL, pH 3.1 and 312 K. Under these conditions, the activity recovery of laccase@CKMB was the highest, reaching 61.78 %. Then, the CKMB and laccase@CKMB were characterized by TGA, FT-IR, XRD, BET and SEM, and the results showed that laccase could be well immobilized on CKMB, the maximum enzyme loading could reach 57.5 mg/g. Compared to free laccase, the storage and pH stability of laccase@CKMB was improved greatly. The laccase@CKMB retained about 40 % of relative activity (4 ℃, 30 days) and more than 50 % of relative activity at pH 2-6. In addition, the laccase@CKMB indicated the reusability up to 6 reaction cycles while retaining 45.1 % of relative activity. Moreover, the thermal deactivation kinetic studies of laccase@CKMB showed a lower k value (0.00275 min-1) and higher t1/2 values (252.0 min) than the k value (0.00573 min-1) and t1/2 values (121.0 min) of free laccase. Conclusions: We explored scientific and reasonable immobilization conditions of laccase@CKMB, and the laccase@CKMB possessed relatively better stabilities, which gave the immobilization of laccase on this cheap and easily available carrier material the possibility of industrial applications.

show abstract

Dynamic of the structural alteration of biochar in ancient Anthrosol over a long timescale by Raman spectroscopy

Cited by 29 publications

References 60 publications

The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar

The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar

A holistic overview on corn cob biochar: A mini-review

The Study of Laccase Immobilization Optimization and Stability Improvement on CTAB-KOH Modified Biochar

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