Dynamic of H5N1 virus in Cambodia and emergence of a novel endemic sub-clade

Sorn, San; Touch, Sok; Ly, Sovann; Rith, Sareth; Nguyen, Tung; Viari, Alain; Gavotte, Laurent; Holl, Davun; Heng, Seng; Asgari, Nima; Richner, Beat; Laurent, Denis; Chea, Nora; Duong, Veasna; Toyoda, Tetsuya; Yasuda, Chadwick Y.; Kitsutani, Paul; Zhou, Paul; Sun, Bing; Denis, Vincent; Donis, Ruben O.; Frutos, Roger; Buchy, Philippe

doi:10.1016/j.meegid.2012.05.013

Cited by 26 publications

(31 citation statements)

References 25 publications

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“…We recently described a novel ADCC assay that measures antibody-mediated NK cell activation (23), developed as modifications of human immunodeficiency virus (HIV)-specific ADCC assays (24,25). This influenza virus ADCC assay was subsequently adapted to study macaque samples.…”

Section: Methodsmentioning

confidence: 99%

Standard Trivalent Influenza Virus Protein Vaccination Does Not Prime Antibody-Dependent Cellular Cytotoxicity in Macaques

Jegaskanda

Amarasena

Laurie

et al. 2013

J Virol

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Yearly vaccination with the trivalent inactivated influenza vaccine (TIV) is recommended, since current vaccines induce little cross neutralization to divergent influenza strains. Whether the TIV can induce antibody-dependent cellular cytotoxicity (ADCC) responses that can cross-recognize divergent influenza virus strains is unknown. We immunized 6 influenza-naive pigtail macaques twice with the 2011-2012 season TIV and then challenged the macaques, along with 12 control macaques, serially with H1N1 and H3N2 viruses. We measured ADCC responses in plasma to a panel of H1 and H3 hemagglutinin (HA) proteins and influenza virus-specific CD8 T cell (CTL) responses using a sensitive major histocompatibility complex (MHC) tetramer reagent. The TIV was weakly immunogenic and, although binding antibodies were detected by enzyme-linked immunosorbent assay (ELISA), did not induce detectable influenza virus-specific ADCC or CTL responses. The H1N1 challenge elicited robust ADCC to both homologous and heterologous H1 HA proteins, but not influenza virus HA proteins from different subtypes (H2 to H7). There was no anamnestic influenza virus-specific ADCC or CTL response in vaccinated animals. The subsequent H3N2 challenge did not induce or boost ADCC either to H1 HA proteins or to divergent H3 proteins but did boost CTL responses. ADCC or CTL responses were not induced by TIV vaccination in influenza-naive macaques. There was a marked difference in the ability of infection compared to that of vaccination to induce cross-reactive ADCC and CTL responses. Improved vaccination strategies are needed to induce broad-based ADCC immunity to influenza.

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Section: Methodsmentioning

confidence: 99%

Standard Trivalent Influenza Virus Protein Vaccination Does Not Prime Antibody-Dependent Cellular Cytotoxicity in Macaques

Jegaskanda

Amarasena

Laurie

et al. 2013

J Virol

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“…Nucleotide sequence analysis of the HA genes revealed that all viruses belong to the clade 1.1.2 phylogenetic group (9,10). While all 2013 data from neighboring countries may not be available, the Cambodian viruses clustered in a discrete group made up of samples collected only in Cambodia during 2013 (Fig.…”

Section: E Arly Detection Of Avian Influenza a Virus Infection In Humansmentioning

confidence: 99%

Identification of Molecular Markers Associated with Alteration of Receptor-Binding Specificity in a Novel Genotype of Highly Pathogenic Avian Influenza A(H5N1) Viruses Detected in Cambodia in 2013

Rith

Davis

Duong

et al. 2014

J Virol

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Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further.

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“…Both Re-5 and Re-1 vaccines (Re-1, implemented since 2006; of rgA/Goose/Guangdong/1996 of HA clade 0, generated by reverse genetics) conferred a high level of protection (over 90%) in chickens against clade 2.3.2.1a by challenge test in laboratory; and about 70% seropositivity (by HA inhibition test) in immunized poulty in the field. However, protection level of Re-1/Re-5 vaccines, by challenging with strains of novel clade 2.3.2.1 (a, b, c), was much lower in ducks and muscovy ducks [47,49,65, 67,69,73]. …”

Section: Spatial-temporal Transmission Of H5n1 Clades and Dynamic Of mentioning

confidence: 99%

“…Concurrently, strains of more virulent clade 1.1, now evolved into 1.1.1 and 1.1.2, are predominantly present in Mekong Delta provinces, which have been phylogenetically revealed to share close evolution of the Cambodian clade 1 progenitors [42,44,73]. The presence of clade 1.1 and its subclades 1.1.1/1.1.2 in several provinces of Central Vietnam in 2013, indicated that now these clades are heading towards North Vietnam from Mekong Delta Basin.…”

Section: Spatial-temporal Transmission Of H5n1 Clades and Dynamic Of mentioning

confidence: 99%

Evolutionary dynamics of highly pathogenic avian influenza A/H5N1 HA clades and vaccine implementation in Vietnam

Nguyen

2014

Clin Exp Vaccine Res

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Based on hemagglutinin (HA) and neuraminidase (NA), influenza A virus is divided into 18 different HA (H1 to H18) and 11 NA types (N1 to N11), opening the possibility for reassortment between the HA and NA genes to generate new HxNy subtypes (where x could be any HA and y is any NA, possibly). In recent four years, since 2010, highly pathogenic avian influenza (HPAI) viruses of H5N1 subtype (HPAI A/H5N1) have become highly enzootic and dynamically evolved to form multiple H5 HA clades, particularly in China, Vietnam, Indonesia, Egypt, Cambodia, and Bangladesh. So far, after more than 10 years emerged in Vietnam (since late 2003), HPAI A/H5N1 is still posing a potential risk of causing outbreaks in poultry, with high frequency of annual endemics. Intragenic variation (referred to as antigenic drift) in HA (e.g., H5) has given rise to form numerous clades, typically marking the major timelines of the evolutionary status and vaccine application in each period. The dominance of genetically and antigenically diversified clade 2.3.2.1 (of subgroups a, b, c), clade 1.1 (1.1.1/1.1.2) and re-emergence of clade 7.1/7.2 at present, has urged Vietnam to the need for dynamically applied antigenicity-matching vaccines, i.e., the plan of importing Re-6 vaccine for use in 2014, in parallel use of Re-1/Re-5 since 2006. In this review, we summarize evolutionary features of HPAI A/H5N1 viruses and clade formation during recent 10 years (2004-2014). Dynamic of vaccine implementation in Vienam is also remarked.

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Dynamic of H5N1 virus in Cambodia and emergence of a novel endemic sub-clade

Cited by 26 publications

References 25 publications

Standard Trivalent Influenza Virus Protein Vaccination Does Not Prime Antibody-Dependent Cellular Cytotoxicity in Macaques

Standard Trivalent Influenza Virus Protein Vaccination Does Not Prime Antibody-Dependent Cellular Cytotoxicity in Macaques

Identification of Molecular Markers Associated with Alteration of Receptor-Binding Specificity in a Novel Genotype of Highly Pathogenic Avian Influenza A(H5N1) Viruses Detected in Cambodia in 2013

Evolutionary dynamics of highly pathogenic avian influenza A/H5N1 HA clades and vaccine implementation in Vietnam

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