Dynamic of Distribution of Human Bone Marrow-Derived Mesenchymal Stem Cells After Transplantation into Adult Unconditioned Mice

Allers, Carolina; Sierralta, Walter; Rivera, Francisco J.; Minguell, José J.; Conget, Paulette

doi:10.1097/01.tp.0000128334.93343.b3

Cited by 147 publications

(112 citation statements)

References 26 publications

(41 reference statements)

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“…The relationship between expression of telomerase and telomere length stabilization and the extension of the life span of the human cell have been reported. 21,22 MSCs, or marrow stromal cells, are multipotential cells. 2,23 Stromal cells of the marrow microenvironment and their associated biosynthetic products provide the physical framework within which hematopoiesis occurs, synthesize or present growth-stimulatory and growthinhibitory factors, produce numerous extracellular matrix ligands, and express a broad repertoire of adhesion molecules that serve to mediate hematopoietic stem cell homing and to transmit regulatory signals.…”

Section: Discussionmentioning

confidence: 99%

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Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Zhao

Liao

Jiang

et al. 2005

Bone Marrow Transplant

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Summary:Human mesenchymal stem cells (hMSCs) are excellent candidates for ex vivo gene transfer and cell therapy in various systems. However, hMSCs are mortal somatic cells, and thus invariably enter an irreversible growth arrest after a finite number of cell divisions in culture. It has been proposed that this is due to telomere shortening. In this study, pGRN145 plasmid containing human telomerase reverse transcriptase (hTRT) was introduced into fetal dermis-derived hMSCs. Single-cell clones positive for telomerase activity and hTRT mRNA were selected and expanded. Single-cell-derived hTRT þ cells could be expanded rapidly in vitro and passaged up to 70 doublings without showing senescence. FACScan flow cytometer showed that hTRT þ cells were positive for CD29, CD44, CD105, and CD166, while CD31, CD45, CD34, vWF, and HLA-DR were negative. Under suitable conditions, hTRT þ cells have the ability of multiple lineage differentiation, including bone, fat, and nerve. Furthermore, transplantation of hTRT þ cells could protect NOD/SCID mice from lethal irradiation. Thus, these cells may be an ideal cell source for promoting hematopoietic recovery after radiotherapy. Bone Marrow Transplantation (2005) 36, 355-365.

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Section: Discussionmentioning

confidence: 99%

“…Although Conget showed that human MSC can be detected in mouse bone marrow for as long as 1 year after human MSC infusion, we did the 6-month safety test according to the requirement of the authorities for the new medical product. 21 …”

Section: Safety Profile Of Transplanted Htrt þ Mscsmentioning

confidence: 99%

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Zhao

Liao

Jiang

et al. 2005

Bone Marrow Transplant

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“…As a result, minimally invasive treatments such as injecting growth factors to stimulate the NP cells to synthesize proteoglycans [74] or injecting cells able to synthesize appropriate ECM such as bone marrow mesenchymal stem cells [48,67,108] are the most commonly proposed. Nevertheless, one important inadequacy of this approach is that the high disc pressure and the aqueous nature of the cell suspension usually result in depletion of the local availability of cells [5,53]. As a result, use of injectable carriers is necessary to effectively deliver cells into the degenerative NP space.…”

Section: Scaffolding In Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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“…Our data are in good agreement with previously published reports demonstrating that after syngeneic or xenogeneic transplantation of MSCs derived from the bone morrow of mouse, rat or nonhuman primates, donor cells engraft into many tissues of conditioned recipients. [53][54][55][56][57] It is still unclear whether transplanted cells continue to retain their multilineage potentials and clonogenic ability or if they were able to commit into residing tissue. At present, Overall, our results provide evidence supporting the suitability of genetically corrected MSCs by ODN in cellbased therapeutic applications.…”

Section: Discussionmentioning

confidence: 99%

Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells

et al. 2008

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Synthetic oligodeoxynucleotides (ODNs) had been employed in gene modification and represent an alternative approach to 'cure' genetic disorders caused by mutations. To test the ability of ODN-mediated gene repair in bone marrow-derived mesenchymal stem cells (MSCs), we established MSCs cell lines with stably integrated mutant neomycin resistance and enhanced green fluorescent protein reporter genes. The established cultures showed morphologically homogenous population with phenotypic and functional features of mesenchymal progenitors. Transfection with gene-specific ODNs successfully repaired targeted cells resulting in the expression of functional proteins at relatively high frequency approaching 0.2%. Direct DNA sequencing confirmed that phenotype change resulted from the designated nucleotide correction at the target site. The position of the mismatchforming nucleotide was shown to be important structural feature for ODN repair activity. The genetically corrected MSCs were healthy and maintained an undifferentiated state. Furthermore, the genetically modified MSCs were able to engraft into many tissues of unconditioned transgenic mice making them an attractive therapeutic tool in a wide range of clinical applications.

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Dynamic of Distribution of Human Bone Marrow-Derived Mesenchymal Stem Cells After Transplantation into Adult Unconditioned Mice

Abstract: These results suggest that human MSC transplantation into unconditioned recipients represents an option for providing cellular therapy and avoids the complications associated with drugs or radiation conditioning.

Cited by 147 publications

References 26 publications

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells

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