Dynamic Modulation of Enzyme Activity by Near‐Infrared Light

Wang, Changping; Zhang, Qiang; Wang, Xinyu; Chang, Hong; Zhang, Sanjun; Tang, Yuankai; Xu, Jianhua; Qi, Ruijuan; Cheng, Yiyun

doi:10.1002/anie.201700968

Cited by 88 publications

(52 citation statements)

References 50 publications

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“…We suspected that the increased activity of the THE system under light irradiation was due to an increase in local temperature as a result of the photothermal effect of Fe 3 O 4 @rGO. Similar phenomena of enhancing enzymatic activity by anchoring Pt nanoparticles to enzyme molecules was previously reported by Cheng and co‐workers; this enhanced the enzyme activity without increasing bulk temperature. They used near‐infrared light, which allowed deep tissue penetration, and this system could be further used to study and control protein activities in cells and organisms.…”

Section: Figuresupporting

confidence: 82%

Targeted Heating of Enzyme Systems Based on Photothermal Materials

Wang

Zhou

et al. 2019

ChemBioChem

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This study demonstrates that the enzymatic reaction rate can be increased significantly by targeted heating of the microenvironment around the enzyme, while maintaining the reaction system at environmental temperature. Enzyme molecules are covalently attached to the surface of Fe3O4@reduced graphite oxide (rGO). Under visible‐light irradiation, the reaction rate catalyzed by the enzyme–Fe3O4@rGO system is clearly enhanced relative to that of the free enzyme and a mixture of free enzyme and Fe3O4@rGO. This local heating mechanism contributes to promotion of the enzymatic reactions of the targeted heating of the enzyme (THE) system, which has been validated by using different enzymes, including lipase, glucose oxidase, and organophosphorus hydrolase. These results indicate that targeted heating of the catalytic centers has the same effect on speeding up reactions as that of traditional heating methods, which treat the whole reaction system. As an example, it is shown that the THE system promotes the sensitivity of an enzyme screen‐printed electrode by 14 times at room temperature, which implies that the THE system can be advantageous in improving enzyme efficiency, especially if heating the entire system is impossible or could lead to degradation of substrates or damage of components, such as in vitro bioanalysis of frangible molecules or in vivo diagnosis.

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Section: Figuresupporting

confidence: 82%

Targeted Heating of Enzyme Systems Based on Photothermal Materials

Wang

Zhou

et al. 2019

ChemBioChem

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“…Because ultraviolet (UV) or visible light shows shallow tissue penetration depth and has high photon energy that will lead to photodamage, near‐infrared (NIR) light (700–1000 nm) that penetrates deeper into tissues and induces minimal detriments is preferred . NIR light thus can be utilized for molecular imaging, such as afterglow imaging and photoacoustic imaging, phototherapy including photodynamic therapy (PDT), photothermal therapy, and photoimmunotherapy, photoactivation of prodrugs or proinhibitors, and photoregulation of biological events, such as gene expression, enzymatic activity, neural activity, biocatalysis, and so on. However, NIR light‐mediated photoinhibition of intracellular protein biosynthesis has been rarely exploited for cancer therapy.…”

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confidence: 99%

Near‐Infrared Photoactivatable Semiconducting Polymer Nanoblockaders for Metastasis‐Inhibited Combination Cancer Therapy

et al. 2019

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Inhibition of protein biosynthesis is a promising strategy to develop new therapeutic modalities for cancers; however, noninvasive precise regulation of this cellular event in living systems has been rarely reported. In this study, a semiconducting polymer nanoblockader (SPNB) is developed that can inhibit intracellular protein synthesis upon near‐infrared (NIR) photoactivation to synergize with photodynamic therapy (PDT) for metastasis‐inhibited cancer therapy. SPNB is self‐assembled from an amphiphilic semiconducting polymer which is grafted with poly(ethylene glycol) conjugated with a protein biosynthesis blockader through a singlet oxygen (1O2) cleavable linker. Such a designed molecular structure not only enables generation of 1O2 under NIR photoirradiation for PDT, but also permits photoactivation of blockaders to terminate protein translation. Thereby, SPNB exerts a synergistic action to afford an enhanced therapeutic efficacy in tumor ablation. More importantly, SPNB‐mediated photoactivation of protein synthesis inhibition precisely and remotely downregulates the expression levels of metastasis‐related proteins in tumor tissues, eventually contributing to the complete inhibition of lung metastasis. This study thus proposes a photoactivatable protherapeutic design for metastasis‐inhibited cancer therapy.

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“…Zhu et al reported the first example of a temperature-responsive enzyme-polymer nanobiocatalyst, which showed markedly enhanced catalytic activities in organic media at 40 • C [35]. Controlled activation of non-photosensitive enzymatic reactions using a light-to-thermal strategy was also achieved with the help of photosensitive nanostructured supports such as noble metal nanoparticles and semiconductor nanomaterials [83,84]. Blankschien et al prepared a thermophilic enzyme-Au nanorod nanobiocatalyst that showed an improvement of enzymatic reaction rate of about 60% upon photothermal activation [24].…”

Section: Temperature Effectsmentioning

confidence: 99%

Recent Advances in Enzyme-Nanostructure Biocatalysts with Enhanced Activity

Zhang

et al. 2020

Catalysts

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Owing to their unique physicochemical properties and comparable size to biomacromolecules, functional nanostructures have served as powerful supports to construct enzyme-nanostructure biocatalysts (nanobiocatalysts). Of particular importance, recent years have witnessed the development of novel nanobiocatalysts with remarkably increased enzyme activities. This review provides a comprehensive description of recent advances in the field of nanobiocatalysts, with systematic elaboration of the underlying mechanisms of activity enhancement, including metal ion activation, electron transfer, morphology effects, mass transfer limitations, and conformation changes. The nanobiocatalysts highlighted here are expected to provide an insight into enzyme-nanostructure interaction, and provide a guideline for future design of high-efficiency nanobiocatalysts in both fundamental research and practical applications.Catalysts 2020, 10, 338 2 of 15 nanoscale supports, such as nanoparticles, nanowires, microspheres, metal-organic frameworks, and nanoflowers, has also drawn extensive attention in recent years [5,[16][17][18][19][20][21][22]. A great deal of effort has also been made to design nanostructured supports with a variety of components including noble metal (e.g., Au) [23,24], metal oxides (e.g., Cu 2 O, Fe 3 O 4 , SiO 2 , Ti 8 O 15 , alumina) [16,25-33], polymer (e.g., Cu 2+ /PAA/PPEGA matrix, aldehyde-derived Pluronic polymer, polycaprolactone) [34-36], metal-organic frameworks (e.g., zeolitic imidazolate framework) [37-39], carbon based (e.g., carbon dots, carbon nanotubes) [40-44], and complex compounds (e.g., Cu 3 (PO 4 ) 2 ·3H 2 O, Ca 3 (PO 4 ) 2 , Co 3 (PO 4 ) 2 ·8H 2 O, Mn 3 (PO 4 ) 2 , Ca 8 H 2 (PO 4 ) 6 , Cu 4 (OH) 6 )SO 4 , CaHPO 4 , Zn 3 (PO 4 ) 2 , Mg-Al layered double hydroxide, CdSe/ZnS quantum dots) [17,[45][46][47][48][49][50][51][52][53][54][55][56][57][58][59]. These representative supports, which possess unique chemical and physical properties, such as controllable release of ion activator and synergic catalysts and response to external stimuli, are able to regulate the enzyme-support interaction and eventually lead to an unprecedented enhancement in immobilized enzyme activity [7,60,61].Overall, recent years have witnessed great success in the interactions between artificial nanostructured supports and natural enzymes for enhanced activity. This review will focus on recent advances in this field in the past 10 years, with an emphasis on various mechanisms behind the boosted catalytic activities, including reduced mass transfer limitation, interfacial ion activation, local heating effect, synergistic effects, conformational changes, substrate channeling and so on. A better understanding of the relationship between the characteristics of the nanostructured supports and the enhanced activities of nanobiocatalysts, may provide new insights in designing efficient nanobiocatalysts for various applications. Mechanisms behind Enhanced Activities of NanobiocatalystsAn overview of recently reported nanobiocatalyst...

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Dynamic Modulation of Enzyme Activity by Near‐Infrared Light

Cited by 88 publications

References 50 publications

Targeted Heating of Enzyme Systems Based on Photothermal Materials

Targeted Heating of Enzyme Systems Based on Photothermal Materials

Near‐Infrared Photoactivatable Semiconducting Polymer Nanoblockaders for Metastasis‐Inhibited Combination Cancer Therapy

Recent Advances in Enzyme-Nanostructure Biocatalysts with Enhanced Activity

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