Dynamic Migration Modes of Collective Cells

Lin, Shao-Zhen; Sang, Ye; Xu, Guang-Kui; Li, Bo; Feng, Xi‐Qiao

doi:10.1016/j.bpj.2018.09.010

Cited by 73 publications

(95 citation statements)

References 65 publications

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“…Finally, it is worth emphasizing that collective cell migration is also mediated by cell-cell social interactions [26,28], cell-cell adhesion [8,15] and cell-matrix adhesion [58]. We have performed robustness tests on the regulating roles of cell-cell social interactions and cell-cell adhesion in confined collective cell migration (see Material and methods).…”

Section: Discussionmentioning

confidence: 99%

“…Due to the confluency, they form an inter-connected polygonal tiling. The shapes and dynamics of cells are determined from the position of vertices, given by r i (t), which obeys the overdamped equation of motion [26][27][28][29] dr…”

Section: Biophysical Modelmentioning

confidence: 99%

“…where g is the friction coefficient and f U i ¼ À@U=@r i denotes the potential force acting on vertex i. n J refers to the number of connecting neighbours of cell J, and P J[C i computes a summation over all neighbouring cells C i of vertex i. v 0 is the self-propelled velocity accounting for the active motility of cells, and e J ¼ (cos u J , sin u J ) is the polarity vector of cell J. The evolution of polarity direction u J satisfies [28] du…”

Section: Biophysical Modelmentioning

confidence: 99%

“…Interface 16: 20190258 cell re-orientation. The roles of LA and CIL in regulating collective cell migration have been explored previously [26,28].…”

Section: Biophysical Modelmentioning

confidence: 99%

“…A T1 transition means that two originally separated but approaching cells may push out their common neighbours such that they would get in contact [39]. In our simulations, a T1 transition is performed when the distance between the two approaching cells becomes shorter than a threshold D T1 , taken as D T1 ¼ 2:5L=(K a A 0 ) here [28], and the potential energy of the post-transition configuration is lower than that before the transition. Besides, the cells are randomly polarized at the initial state, i.e.…”

Section: Biophysical Modelmentioning

confidence: 99%

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Dynamic instability and migration modes of collective cells in channels

Lin

et al. 2019

J. R. Soc. Interface.

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Migrating cells constantly experience geometrical confinements in vivo , as exemplified by cancer invasion and embryo development. In this paper, we investigate how intrinsic cellular properties and extrinsic channel confinements jointly regulate the two-dimensional migratory dynamics of collective cells. We find that besides external confinement, active cell motility and cell crowdedness also shape the migration modes of collective cells. Furthermore, the effects of active cell motility, cell crowdedness and confinement size on collective cell migration can be integrated into a unified dimensionless parameter, defined as the cellular motility number (CMN), which mirrors the competition between active motile force and passive elastic restoring force of cells. A low CMN favours laminar-like cell flows, while a high CMN destabilizes cell motions, resulting in a series of mode transitions from a laminar phase to an ordered vortex chain, and further to a mesoscale turbulent phase. These findings not only explain recent experiments but also predict dynamic behaviours of cell collectives, such as the existence of an ordered vortex chain mode and the mode selection under non-straight confinements, which are experimentally testable across different epithelial cell lines.

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Section: Discussionmentioning

confidence: 99%

Section: Biophysical Modelmentioning

confidence: 99%

Section: Biophysical Modelmentioning

confidence: 99%

“…Interface 16: 20190258 cell re-orientation. The roles of LA and CIL in regulating collective cell migration have been explored previously [26,28].…”

Section: Biophysical Modelmentioning

confidence: 99%

Section: Biophysical Modelmentioning

confidence: 99%

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Dynamic instability and migration modes of collective cells in channels

Lin

et al. 2019

J. R. Soc. Interface.

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Matrix Anisotropy Promotes a Transition of Collective to Disseminated Cell Migration via a Collective Vortex Motion

Matsubara

et al. 2023

Advanced Biology

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Cells detached and disseminated away from collectively migrating cells are frequently found during tumor invasion at the invasion front, where extracellular matrix (ECM) fibers are parallel to the cell migration direction. However, it remains unclear how anisotropic topography promotes the transition of collective to disseminated cell migration. This study applies a collective cell migration model with and without 800 nm wide aligned nanogrooves parallel, perpendicular, or diagonal to the cell migration direction. After 120 hour migration, MCF7‐GFP‐H2B‐mCherry breast cancer cells display more disseminated cells at the migration front on parallel topography than on other topographies. Notably, a fluid‐like collective motion with high vorticity is enhanced at the migration front on parallel topography. Furthermore, high vorticity but not velocity is correlated with disseminated cell numbers on parallel topography. Enhanced collective vortex motion colocalizes with cell monolayer defects where cells extend protrusions into the free space, suggesting that topography‐driven cell crawling for defect closure promotes the collective vortex motion. In addition, elongated cell morphology and frequent protrusions induced by topography may further contribute to the collective vortex motion. Overall, a high‐vorticity collective motion at the migration front promoted by parallel topography suggests a cause of the transition of collective to disseminated cell migration.

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Collective Organization Behaviors of Multi‐Cell Systems Induced by Engineered ECM‐Cell Mechanical Coupling

Wang

Zheng

et al. 2023

Adv Funct Materials

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Cells in vivo are surrounded by fibrous extracellular matrix (ECM), which can mediate the propagation of active cellular forces through stressed fiber bundles and regulate various biological processes. However, the mechanisms for multi‐cellular organization and collective dynamics induced by cell‐ECM mechanical couplings, which are crucial for the development of novel ECM‐based biomaterial for cell manipulation and biomechanical applications, remain poorly understood. Herein, the authors design an in vitro quasi‐3D experimental system and demonstrate a transition between spreading and aggregating in collective organizational behaviors of discrete multi‐cellular systems, induced by engineered ECM‐cell mechanical coupling, with the observed phenomena and underlying mechanisms differing fundamentally from those of cell monolayers. During the process of collective cell organization, the collagen substrate undergoes reconstruction into a dense fiber network structure, which is correlated with local cellular density and consistent with observed enhanced cells' motility; and the weakening of fiber bundle formation within the hydrogel reduces cells’ movement. Moreover, cells can respond to the curvature and shape of the original cell population and form different aggregation patterns. These results elucidate important physical factors involved in collective cell organization and provide important references for potential applications of biomaterials in new therapies and tissue engineering.

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Dynamic Migration Modes of Collective Cells

Cited by 73 publications

References 65 publications

Dynamic instability and migration modes of collective cells in channels

Dynamic instability and migration modes of collective cells in channels

Matrix Anisotropy Promotes a Transition of Collective to Disseminated Cell Migration via a Collective Vortex Motion

Collective Organization Behaviors of Multi‐Cell Systems Induced by Engineered ECM‐Cell Mechanical Coupling

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