Dynamic light scattering from collagen solutions. II. Photon correlation study of the depolarized light

Thomas, John C.; Fletcher, Guy C.

doi:10.1002/bip.1979.360180603

Cited by 41 publications

(15 citation statements)

References 33 publications

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“…The midpoint of the secondary bound transition in the three-strand complex is T, = 39°C. In this calculation the cooperativity parameter was u = 5 X Because aggregation was so clearly manifested in the observations of Thomas and Fletcher,16 one may use the present calculations to infer that u for collagen must be about 5 X lop3, or larger. Certainly, u cannot be as small as 1 X (Recall, though, that the elementary bond unit here is the tripeptide.…”

Section: Collagenmentioning

confidence: 95%

A theory of aggregation in the thermal denaturation region of multistrand biopolymers

Shibata

Schurr

1981

Biopolymers

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SynopsisA theory explicitly allowing the possibility of aggregation of multistrand hiopolymers is proposed. It is found that the same secondary bonds responsible for stabilizing the native structure a t low temperature will promote aggregation in the thermal denaturation region for sufficiently long chains. A requirement for both open and zippered regions dictates that.the aggregation region does not extend far below T,. However, its width, or extension on the high-temperature side of T,, is a strongly increasing function of chain length and also of the cooperativity parameter. The present theoretical results obtained for DNA and collagen with almost no adjustable parameters are in good qualitative agreement with a number of previously poorly understood experimental observations. The significance of such a spontaneous aggregation phenomenon for genetic recombination is noted.

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Section: Collagenmentioning

confidence: 95%

A theory of aggregation in the thermal denaturation region of multistrand biopolymers

Shibata

Schurr

1981

Biopolymers

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“…Obrink, 1972). While there has been great progress in characterizing mechanical properties of molecules, many researchers found collagen has some flexibility (Fletcher, 1976;Thomas and Fletcher, 1979;George and Veis, 1991;Shattuck et al, 1994). Due to intrinsic limitations of various methods and assumptions in the models, however, the conflicting results were obtained using different methods to quantify the flexibility of collagen molecules (Utiyama et al, 1973;Saito et al, 1982;Nestler et al, 1983;Hofmann et al, 1984;Sun et al, 2002).…”

Section: Introductionmentioning

confidence: 98%

Stretching type II collagen with optical tweezers

Sun

Luo

Fertala

et al. 2004

Journal of Biomechanics

126

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“…(O) (5) ffb) = ct(.r)/ct(o) (6) From Eqs. (l), (a), (5), and (6) we obtain and after normalization with where A and B are constants, independent of the concentration of scatterers.…”

Section: )mentioning

confidence: 98%

Influence of double scattering in determination of rotational diffusion coefficients by depolarized dynamic light scattering: Application to the bacteriophages T7 and T4B

1980

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SynopsisDynamic light scattering experiments were performed on solutions of the bacteriophages T7 and T4B in order to obtain the rotational diffusion coefficients of these phages. Correlation functions were determined from the depolarized intensity scattered in the forward direction. The apparatus used in this study is described in detail. Particular attention is paid to the minimalization of the depolarized intensity due to double scattering. If double scattering cannot he neglected, the correlation function of the depolarized field is the sum of the correlation functions resulting from single and double scattering. It is shown that by correcting for double scattering, it is then possible to obtain the rotational diffusion coefficient of the macromolecules. Although the optical anisotropy of both T4R (retracted fihers) and T7 is very small, the experimental conditions could be chosen in such a way that no depolarized scattering due to double scattering was observed. The measured rotational diffusion coefficients for T4B and T7 are D R~,~ = 258 f 12 and 4528 f 100 sec-', respectively. These values compare very well with those obtained by electric birefringence experiments.

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Dynamic light scattering from collagen solutions. II. Photon correlation study of the depolarized light

Cited by 41 publications

References 33 publications

A theory of aggregation in the thermal denaturation region of multistrand biopolymers

A theory of aggregation in the thermal denaturation region of multistrand biopolymers

Stretching type II collagen with optical tweezers

Influence of double scattering in determination of rotational diffusion coefficients by depolarized dynamic light scattering: Application to the bacteriophages T7 and T4B

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