Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos

Darnell, Diana K.; García‐Martínez, Virginio; López‐Sánchez, Carmen; Yuan, Shipeng; Schoenwolf, Gary C.

doi:10.1385/1-59259-685-1:305

Cited by 17 publications

(23 citation statements)

References 10 publications

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“…WA09 (WiCell), RUES1, RUES2 (A. Brivanlou, The Rockefeller University, New York) hESCs, and the hiPSC lines Fib2-iPS4 and Fib2-iPS5 (George Daley, Children's Hospital, Boston) were cultured on Geltrex-coated plates (Invitrogen) in chemically defined media containing Heregulin β (10 ng/mL), Activin A (10 ng/mL), LR-Igf (200 ng/mL), and Fgf2 (8 ng/mL) as described previously (24).…”

Section: Methodsmentioning

confidence: 99%

Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells

Menéndez

Yatskievych

Antin

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

236

308

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Neural crest stem cells can be isolated from differentiated cultures of human pluripotent stem cells, but the process is inefficient and requires cell sorting to obtain a highly enriched population. No specific method for directed differentiation of human pluripotent cells toward neural crest stem cells has yet been reported. This severely restricts the utility of these cells as a model for disease and development and for more applied purposes such as cell therapy and tissue engineering. In this report, we use small-molecule compounds in a single-step method for the efficient generation of self-renewing neural crest-like stem cells in chemically defined media. This approach is accomplished directly from human pluripotent cells without the need for coculture on feeder layers or cell sorting to obtain a highly enriched population. Critical to this approach is the activation of canonical Wnt signaling and concurrent suppression of the Activin A/Nodal pathway. Over 12–14 d, pluripotent cells are efficiently specified along the neuroectoderm lineage toward p75 + Hnk1 + Ap2 + neural crest-like cells with little or no contamination by Pax6 + neural progenitors. This cell population can be clonally amplified and maintained for >25 passages (>100 d) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells, and mesenchymal precursor cells. Neural crest-like stem cell-derived mesenchymal precursors have the capacity for differentiation into osteocytes, chondrocytes, and adipocytes. In sum, we have developed methods for the efficient generation of self-renewing neural crest stem cells that greatly enhance their potential utility in disease modeling and regenerative medicine.

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Section: Methodsmentioning

confidence: 99%

Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells

Menéndez

Yatskievych

Antin

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

236

308

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“…Method has been described previously 29,58 . Fluorescent dye injection was performed on 8-ss chick embryos.…”

Section: Methodsmentioning

confidence: 99%

Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling

et al. 2012

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Neural crest cells constitute a multipotent cell population that gives rise to diverse cell lineages. The neural crest arising from the postotic hindbrain is known as the 'cardiac' neural crest, and contributes to the great vessels and outflow tract endocardial cushions, but the neural crest contribution to structures within the heart remains largely controversial. Here we demonstrate that neural crest cells from the preotic region migrate into the heart and differentiate into coronary artery smooth muscle cells. Preotic neural crest cells preferentially distribute to the conotruncal region and interventricular septum. Ablation of the preotic neural crest causes abnormalities in coronary septal branch and orifice formation. Mice and chicks lacking endothelin signalling show similar abnormalities in the coronary artery, indicating its involvement in neural crest-dependent coronary artery formation. This is the first report that reveals the preotic neural crest contribution to heart development and smooth muscle heterogeneity within a coronary artery.

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“…Chick and quail embryos were used in this study, following different procedures in accordance with our protocols described previously (Darnell et al, 2000;Lopez-Sanchez et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…Two antibodies were used for immunocytochemistry to determine the location of the transplanted quail cells: QH1 (which identifies quail endothelial and endocardial cells) and antiquail antibody (Darnell et al, 2000).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Movement and commitment of primitive streak precardiac cells during cardiogenesis

López‐Sánchez¹,

Garcia-Masa²,

Gañan³

et al. 2009

Int. J. Dev. Biol.

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Fate maps are required to address questions about the commitment and differentiation of precardiac cells. Here, we report a detailed study of the precardiac cells located at the level of the primitive streak, employing different experiments with a variety of techniques combining double transplantations, microinjections and immunocytochemistry. Most cells of the more rostral segments of the primitive streak were found to contribute cells to the endodermal layer, adjacent to precardiac mesodermal cells of the heart forming region whose provenance was in the immediately more caudal segments of the primitive streak. We established a close spatiotemporal relationship between the two cell layers and the expression of their specific cardiac markers (cNkx-2.5, Bmp2, Cripto, Usmaar, dHand, GATA4, Pitx2, Hex, Fgf8, AMHC1 and VMHC1). We also analyzed the ability of precardiac cells to differentiate when they are transplanted to ectopic locations or are subjected to the influence of the organizer. We propose that the precardiac cells of the primitive streak form at least two groups with different significance. One, regulated by mediation of the organizer, is located preferentially in the more rostral region of the primitive streak. It consists of the prospective cells of the endoderm layer, with a hierarchic pattern of expression of different genes characterized by its capacity for induction and regulation of a second group of cells. This second group is located preferentially in the more caudal segments, and is fated to form the precardiac mesoderm, whose differentiation would be characterized by the expression of various specific genes.

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Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos

Cited by 17 publications

References 10 publications

Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells

Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells

Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling

Movement and commitment of primitive streak precardiac cells during cardiogenesis

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