Dynamic immune response characteristics of piglets infected with Actinobacillus pleuropneumoniae through omic

Abstract

Porcine infectious pleuropneumonia is characterized by a high-rate of carriage and mixed infection with other pathogens. The host immune response induced by Actinobacillus pleuropneumoniae (APP) is the basis for elucidating pathogenesis and controlling disease. However, there is currently no comprehensive and dynamic data characterising the host immune response. In this study, piglets were infected with APP and differentially expressed proteins of bronchoalveolar lavage fluid (BALF) and peripheral serum were identified by iTRAQ-LC-MS/MS, and differentially expressed genes of peripheral blood mononuclear cells (PBMC) by RNA-seq. The results of the integrated analysis of serum, BALF and PBMC showed significant metabolism and local immune responses in BALF, the general immune response in PBMC mainly involves cytokines, while that in serum mainly involves biosynthesis, phagosome, and complement and coagulation cascades. Furthermore, immune responses in PBMCs and serum were rapid and maintained compared to the lung where metabolism and cell adhesion activities were enriched. Some innate immunity pathways of the cellular response to ROS, neutrophil mediated immunity, granulocyte activation and leukocyte cell-cell adhesion were identified as central points, connecting multiple signaling pathways to form an integrated large network. At 24 h post-infection, 14 molecules were up regulated in BALF, 10 of which were shared with PBMC, but at 120 h, 20 down-regulated molecules were identified in BALF, 11 of them still up- regulated in PBMC. We conclude that, the immune response in the lung is different from that in blood, but there is a similarity in response in PBMC and serum.

Evaluation the kill rate and mutant selection window of danofloxacin against Actinobacillus pleuropneumoniae in a peristaltic pump model

“…Chronic disease causes a decline in the pig's feed intake, which slows down pig weight gain and seriously affects production in the pig breeding industry [4]. To date, the disease has spread to all regions of the world, seriously affecting pig production and causing huge economic losses to the breeding industry, consequently attracting increased research attention [5][6][7]. However, there are more than 19 serovars of A. pleuropneumoniae; therefore, the protective effect of the currently available vaccine is not satisfactory [8].…”

Evaluation of the mutant selection window of danofloxacin against Actinobacillus pleuropneumoniae in an in vitro dynamic model

“…The clinical symptoms are fibrinous hemorrhagic pneumonia and necrotizing pneumonia (1)(2)(3). AP infection seriously affects development of the pig industry, and can result in considerable economic losses for farmers (4)(5)(6)(7). The common prevention and treatment methods for AP infection are vaccination and drug therapy.…”