Dynamic gut microbiome changes following regional intestinal lymphatic obstruction in primates

Abstract

The pathogenesis of inflammatory bowel disease (IBD) has been linked with lymphostasis, but whether and how lymphatic obstruction might disturb the intestinal microbiome in the setting of Crohn's Disease (CD) is currently unknown. We employed a new model of CD in African Green monkeys, termed 'ATLAS' (African green monkey truncation of lymphatics with obstruction and sclerosis), to evaluate how gut lymphatic obstruction alters the intestinal microbiome at 7, 21 and 61 days. Remarkable changes in several microbial sub-groupings within the gut microbiome were observed at 7 days post-ATLAS compared to controls including increased abundance of Prevotellaceae and Bacteroidetes-Prevotella-Porphyromonas (BPP), which may contribute to disease activity in this model of gut injury. To the best of our knowledge, these findings represent the first report linking lymphatic structural/gut functional changes with alterations in the gut microbiome as they may relate to the pathophysiology of CD.

Intestinal Lymphatic Dysfunction in Kidney Disease

“…99 In a primate model of Crohn disease, obstruction of lymphatic outflow dramatically altered intestinal microbial subgroups, including greater abundance of Prevotellaceae and Bacteroidetes-Prevotella-Porphyromonas. 100 These results illustrate a cycle whereby metabolic changes associated with disease, including kidney disease, disrupt the intestinal microbiome, which impairs the lymphatic vascular network, which, in…”

Human microbiomes in cancer development and therapy

“…This was the first human study to link changes in lymphatic structure/intestinal function and the gut microbiome. 170 Subsequent studies found that the incidence of H. pylori ‐negative mucosa‐associated lymphatic tissue (MALT) lymphoma was related to the presence of H. pylori in some patients. Interestingly, alpha diversity was significantly reduced in H. pylori ‐negative MALT lymphoma patients compared to controls ( p = 0.04).…”

“…In a study on the relationship between changes in human lymphatic structure/intestinal function and gut microbes, researchers determined that the abundance of Prevotella and Bacteroidetes‐Prevotella‐porphyromona (BPP) increased in the gut microbiome. This was the first human study to link changes in lymphatic structure/intestinal function and the gut microbiome 170 . Subsequent studies found that the incidence of H. pylori ‐negative mucosa‐associated lymphatic tissue (MALT) lymphoma was related to the presence of H. pylori in some patients.…”