Dynamic Generation of Concentration- and Temporal-Dependent Chemical Signals in an Integrated Microfluidic Device for Single-Cell Analysis

Gonzalez-Suarez, Alan M.; Castillo, Johanna G. Peña-del; Hernández‐Cruz, Arturo; García-Cordero, José L.

doi:10.1021/acs.analchem.8b02442

Cited by 23 publications

(20 citation statements)

References 32 publications

(51 reference statements)

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“…A one-channel microfluidic device was designed in AutoCAD (Student version, Autodesk) and fabricated by soft-lithography [19]. The device consists of an inlet, an outlet, and a long serpentine channel with a height and width of 20 and 40 μm, respectively.…”

Section: Methodsmentioning

confidence: 99%

Facile assembly of an affordable miniature multicolor fluorescence microscope made of 3D-printed parts enables detection of single cells

Tristan-Landin¹,

Gonzalez-Suarez²,

Jimenez-Valdes³

et al. 2019

Preprint

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18Fluorescence microscopy is one of the workhorses of biomedical research and laboratory diagnosis;19 however, their cost, size, maintenance, and fragility has prevented their adoption in developing countries 20 or low-resource settings. Although significant advances have decreased their size, cost and accessibility, 21 their designs and assembly remain rather complex. Here, inspired on the simple mechanism from a nut and 22 a bolt we report the construction of a portable fluorescence microscope that operates in bright field mode 23 and in three fluorescence channels: UV, green, and red. It is assembled in under 10 min from only six 3D 24printed parts and basic electronic components that can be readily purchased in most locations or online for 25 US $85. Adapting a microcomputer and a touch LCD screen, the microscope can capture time-lapse images 26 and videos. We characterized its resolution and illumination conditions and benchmarked its performance 27 against a high-end fluorescence microscope by tracking a biological process in single cells. We also 28 demonstrate its application to image cells inside a microfluidic device in bright-field and fluorescence 29 mode. Our microscope fits in a CO 2 chamber and can be operated in time-lapse mode. Our portable 30 microscope is ideal in applications where space is at a premium, such as lab-on-a-chips or space missions, 31and can find applications in clinical research, diagnostics, telemedicine and in educational settings. 32 33 3 34

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Section: Methodsmentioning

confidence: 99%

Facile assembly of an affordable miniature multicolor fluorescence microscope made of 3D-printed parts enables detection of single cells

Tristan-Landin¹,

Gonzalez-Suarez²,

Jimenez-Valdes³

et al. 2019

Preprint

Self Cite

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“…S8) and soft lithography methods (Fig. S9) 39 . The singlechamber device consisted of two PDMS layers, the top layer containing flow channels and a cell culture chamber and a bottom layer containing an array of 19 microwells.…”

Section: Fabrication Of a Single Chamber Microfluidic Devicementioning

confidence: 99%

A microfluidic platform for cultivating ovarian cancer spheroids and testing their responses to chemotherapies

et al. 2020

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There is increasing interest in utilizing in vitro cultures as patient avatars to develop personalized treatment for cancer. Typical cultures utilize Matrigel-coated plates and media to promote the proliferation of cancer cells as spheroids or tumor explants. However, standard culture conditions operate in large volumes and require a high concentration of cancer cells to initiate this process. Other limitations include variability in the ability to successfully establish a stable line and inconsistency in the dimensions of these microcancers for in vivo drug response measurements. This paper explored the utility of microfluidics in the cultivation of cancer cell spheroids. Six patient-derived xenograft (PDX) tumors of high-grade serous ovarian cancer were used as the source material to demonstrate that viability and epithelial marker expression in the microfluidic cultures was superior to that of Matrigel or large volume 3D cultures. To further demonstrate the potential for miniaturization and multiplexing, we fabricated multichamber microfluidic devices with integrated microvalves to enable serial seeding of several chambers followed by parallel testing of several drug concentrations. These valve-enabled microfluidic devices permitted the formation of spheroids and testing of seven drug concentrations with as few as 100,000 cancer cells per device. Overall, we demonstrate the feasibility of maintaining difficul-to-culture primary cancer cells and testing drugs in a microfluidic device. This microfluidic platform may be ideal for drug testing and personalized therapy when tumor material is limited, such as following the acquisition of biopsy specimens obtained by fine-needle aspiration.

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“…The plasma treatment oxidizes both surfaces and, upon contact, creates an airtight seal for the fluid microchannels [30]. Several PDMS layers can be assembled to create a more complex microfluidic device for different applications [31]. In recent years, the field of microfluidics has grown rapidly, and the advances made have generated great research interest in biology and medicine.…”

Section: H L I I G G H H T Smentioning

confidence: 99%

Prospects and Opportunities for Microsystems and Microfluidic Devices in the Field of Otorhinolaryngology

Hwang

Gonzalez-Suarez

Stybayeva

et al. 2021

Clin Exp Otorhinolaryngol

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Microfluidic systems can be used to control picoliter to microliter volumes in ways not possible with other methods of fluid handling. In recent years, the field of microfluidics has grown rapidly, with microfluidic devices offering possibilities to impact biology and medicine. Microfluidic devices populated with human cells have the potential to mimic the physiological functions of tissues and organs in a three-dimensional microenvironment and enable the study of mechanisms of human diseases, drug discovery and the practice of personalized medicine. In the field of otorhinolaryngology, various types of microfluidic systems have already been introduced to study organ physiology, diagnose diseases, and evaluate therapeutic efficacy. Therefore, microfluidic technologies can be implemented at all levels of otorhinolaryngology. This review is intended to promote understanding of microfluidic properties and introduce the recent literature on application of microfluidic-related devices in the field of otorhinolaryngology.

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Dynamic Generation of Concentration- and Temporal-Dependent Chemical Signals in an Integrated Microfluidic Device for Single-Cell Analysis

Cited by 23 publications

References 32 publications

Facile assembly of an affordable miniature multicolor fluorescence microscope made of 3D-printed parts enables detection of single cells

Facile assembly of an affordable miniature multicolor fluorescence microscope made of 3D-printed parts enables detection of single cells

A microfluidic platform for cultivating ovarian cancer spheroids and testing their responses to chemotherapies

Prospects and Opportunities for Microsystems and Microfluidic Devices in the Field of Otorhinolaryngology

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