Dynamic expression of neurexophilin1 during zebrafish embryonic development

Thomas-Jinu, Swapna; Houart, Corinne

doi:10.1016/j.gep.2013.07.006

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…Whole-mount in situ hybridization was performed as described elsewhere ( Thomas-Jinu and Houart, 2013 ). The following antisense RNA probes were used: pax2.1 ( Krauss et al., 1991 ), fgf8 ( Reifers et al., 1998 ), foxb1.2 ( Amoyel et al., 2005 , Nakada et al., 2006 ), EphA4 17 , zash1a ( Allende and Weinberg, 1994 ), rfng ( Cheng et al., 2004 ), lhx5 ( Toyama et al., 1995 ) and axin2 ( Carl et al., 2007 ).…”

Section: Methodsmentioning

confidence: 99%

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development

Thomas-Jinu

Gordon

Fielding

et al. 2017

Neuron

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SummaryRecent progress revealed the complexity of RNA processing and its association to human disorders. Here, we unveil a new facet of this complexity. Complete loss of function of the ubiquitous splicing factor SFPQ affects zebrafish motoneuron differentiation cell autonomously. In addition to its nuclear localization, the protein unexpectedly localizes to motor axons. The cytosolic version of SFPQ abolishes motor axonal defects, rescuing key transcripts, and restores motility in the paralyzed sfpq null mutants, indicating a non-nuclear processing role in motor axons. Novel variants affecting the conserved coiled-coil domain, so far exclusively found in fALS exomes, specifically affect the ability of SFPQ to localize in axons. They broadly rescue morphology and motility in the zebrafish mutant, but alter motor axon morphology, demonstrating functional requirement for axonal SFPQ. Altogether, we uncover the axonal function of the splicing factor SFPQ in motor development and highlight the importance of the coiled-coil domain in this process.Video Abstract

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Section: Methodsmentioning

confidence: 99%

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development

Thomas-Jinu

Gordon

Fielding

et al. 2017

Neuron

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“…Standard procedures were followed for in situ hybridisation analysis using full-length probes ( Thomas-Jinu and Houart, 2013 ). For whole-mount in situ hybridisation followed by detection of ectopically expressed GFP-tagged proteins, embryos were incubated in Fast Red substrate solution [buffered in 0.1 M Tris-HCl, 100 mM NaCl (pH 8.2); Roche] for at least 2 h at room temperature (or at 4°C overnight).…”

Section: Methodsmentioning

confidence: 99%

Pineal progenitors originate from a non-neural territory limited by FGF signalling

et al. 2019

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The embryonic development of the pineal organ, a neuroendocrine gland on top of the diencephalon, remains enigmatic. Classic fatemapping studies suggested that pineal progenitors originate from the lateral border of the anterior neural plate. We show here, using gene expression and fate mapping/lineage tracing in zebrafish, that pineal progenitors originate, at least in part, from the non-neural ectoderm. Gene expression in chick indicates that this non-neural origin of pineal progenitors is conserved in amniotes. Genetic repression of placodal, but not neural crest, cell fate results in pineal hypoplasia in zebrafish, while mis-expression of transcription factors known to specify placodal identity during gastrulation promotes the formation of ectopic pineal progenitors. We also demonstrate that fibroblast growth factors (FGFs) position the pineal progenitor domain within the non-neural border by repressing pineal fate and that the Otx transcription factors promote pinealogenesis by inhibiting this FGF activity. The non-neural origin of the pineal organ reveals an underlying similarity in the formation of the pineal and pituitary glands, and suggests that all CNS neuroendocrine organs may require a non-neural contribution to form neurosecretory cells.

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“…Our study also suggests an alternative cause of the up-regulated synthesis of serine proteases in which the PBM diet down-regulated neurexophilin 3 (nxpe3), complexin 2 (cplx2) and cGMP-gated cation channel alpha-1 (cnga1). The first two genes, nxpe3 and cplx2, encode proteins essential for trans-synaptic activation and zymogen exocytosis, while the latter also acts as a transmembrane cation transporter and a regulator of membrane potential (Tetreault et al, 2006;Falkowski et al, 2010;Thomas-Jinu and Houart, 2013). The pancreas in fish is highly innervated, with neuronal fibers containing small granular vesicles whose functioning (exocytosis) is triggered by changes in the cytosolic Ca 2 + concentration (Petersen, 2009).…”

Section: R Panicz Et Almentioning

confidence: 99%

Tench (Tinca tinca) high-throughput transcriptomics reveal feed dependent gut profiles

Panicz¹,

Klopp²,

Igielski³

et al. 2017

Aquaculture

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Dynamic expression of neurexophilin1 during zebrafish embryonic development

Cited by 7 publications

References 18 publications

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development

Pineal progenitors originate from a non-neural territory limited by FGF signalling

Tench (Tinca tinca) high-throughput transcriptomics reveal feed dependent gut profiles

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