Dynamic expression of bone morphogenetic protein 4 in reproductive organs of female mice

Tanwar, Pradeep S.; McFarlane, James R.

doi:10.1530/rep-10-0299

Cited by 27 publications

(26 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support of this, we have found that while the expression of BMPRIB was detectable in follicular cells of mouse primordial follicles, FSHR was absent but later appeared in primary follicles. These data together with a previous report in rat (Moore & Shimasaki 2005) contradict previous reports (Meredith et al 1992, Flaws et al 1997 and therefore suggest the presence of a complex interplay between gonadotrophins and other growth factors such as BMPs where direct and indirect cross-signalling regulates ovarian function (Tanwar & McFarlane 2011). The reported decrease in primordial follicles after eCG treatment (Tanwar et al 2008) conflicts with the present result probably due to the manual counting method used in the previous study instead of the automated stereology used in this work.…”

Section: The Role Of Bmps In Ovarian Functioncontrasting

confidence: 97%

“…The antibodies were raised and characterised in our laboratory as described previously (Tanwar & McFarlane 2011). In brief, the synthetic peptides were conjugated to diphtheria toxoid (CSL) and emulsified in Freunds complete adjuvant (Sigma-Aldrich Pty Ltd) for the primary vaccination and in Freunds incomplete adjuvant for booster injections.…”

Section: Antibodiesmentioning

confidence: 99%

“…The antibodies were purified from egg yolks using a combination ammonium sulphate and octanoic acid as described by McKinney & Parkinson (1987). The cross reactivity of BMP2 and BMP7 was !0.5% for the anti-BMP4 antibody and the specificity of the BMPRIB antibody was determined by competitive binding ELISA test against random peptides (Tanwar & McFarlane 2011). The DELTA-BLAST analysis showed that the sequence was specific to mouse BMPRIB with no potential binding to other proteins.…”

Section: Antibodiesmentioning

confidence: 99%

“…BMP4 mRNA is primarily expressed in theca cells, whereas BMPRIB is predominantly expressed in the granulosa cells of rat ovaries (Shimasaki et al 1999, Erickson & Shimasaki 2003, Tanwar & McFarlane 2011, indicating a paracrine mode of action. BMPRIB is the main and common receptor used by several intraovarian BMPs such as BMP 2, 4, 7, and 15 .…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

The impact of passive immunisation against BMPRIB and BMP4 on follicle development and ovulation in mice

Al-Samerria¹,

Al-Ali²,

McFarlane³

et al. 2015

Reproduction

View full text Add to dashboard Cite

The primordial follicle reserve is the corner stone of female fertility and determines the longevity and quality of reproduction. Complete depletion of this reserve will lead to primary infertility, and the key-limiting step of follicle depletion is the transition from primordial to primary follicles. It has been reported that this process is gonadotrophin-independent, but other conflicting reports are indicated otherwise and this discrepancy needs to be unequivocally clarified. The aim of this study was to investigate the role of bone morphogenetic proteins (BMPs) in the regulation of folliculogenesis in mice passively immunised against BMP receptor 1B (BMPRIB) and BMP4. While a stereological study revealed that the numbers of primordial follicles in immunised mice were significantly higher when compared with control animals, treatment with equine chorionic gonadotrophin showed no effect. In parallel, immunofluorescence microscopy revealed the presence of BMPRIB but not FSH receptor in primordial follicles. The number of primary follicles in immunised mice were also significantly increased when compared with control animals. After puberty, the rates of depletion of primordial and primary follicles were increased with age, particularly in treated animals; however, there was no significant difference between the treatment groups of the same age. Based on these results together with our previous reports in sheep and mice, we confirm that the attenuation of BMP signalling system can be an effective approach to sustain the primordial follicle reserve while promoting the development of growing follicles, ovulation and consequently overall female fertility.

show abstract

Section: The Role Of Bmps In Ovarian Functioncontrasting

confidence: 97%

Section: Antibodiesmentioning

confidence: 99%

Section: Antibodiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

The impact of passive immunisation against BMPRIB and BMP4 on follicle development and ovulation in mice

Al-Samerria¹,

Al-Ali²,

McFarlane³

et al. 2015

Reproduction

View full text Add to dashboard Cite

show abstract

“…BMPR1B is expressed from secondary to atretic follicles and is the receptor for BMP2, -4, -6, and -7. BMPR1B gene KO and mutations have an adverse effect on fertility (compromised cumulus expansion) in mice and in humans (Tanwar & McFarlane 2011). This gene, which responds to BMP15, is also a good candidate for a granulosa signaling back to the oocyte.…”

Section: Gene Analysismentioning

confidence: 99%

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Nivet¹,

Vigneault²,

Blondin³

et al. 2013

Reproduction

View full text Add to dashboard Cite

One of the challenges in mammalian reproduction is to understand the basic physiology of oocyte quality. It is believed that the follicle status is linked to developmental competence of the enclosed oocyte. To explore the link between follicles and competence in cows, previous research at our laboratory has developed an ovarian stimulation protocol that increases and then decreases oocyte quality according to the timing of oocyte recovery post-FSH withdrawal (coasting). Using this protocol, we have obtained the granulosa cells associated with oocytes of different qualities at selected times of coasting. Transcriptome analysis was done with Embryogene microarray slides and validation was performed by real-time PCR. Results show that the major changes in gene expression occurred from 20 to 44 h of coasting, when oocyte quality increases. Secondly, among upregulated genes (20-44 h), 25% were extracellular molecules, highlighting potential granulosa signaling cascades. Principal component analysis identified two patterns: one resembling the competence profile and another associated with follicle growth and atresia. Additionally, three major functional changes were identified: i) the end of follicle growth (BMPR1B, IGF2, and RELN), involving interactions with the extracellular matrix (TFPI2); angiogenesis (NRP1), including early hypoxia, and potentially oxidative stress (GFPT2, TF, and VNN1) and ii) apoptosis (KCNJ8) followed by iii) inflammation (ANKRD1). This unique window of analysis indicates a progressive hypoxia during coasting mixed with an increase in apoptosis and inflammation. Potential signaling pathways leading to competence have been identified and will require downstream testing. This preliminary analysis supports the potential role of the follicular differentiation in oocyte quality both during competence increase and decrease phases.Reproduction (2013) 145 555-565

show abstract

Effects of BMP4/SMAD signaling pathway on mouse primordial follicle growth and survival via up‐regulation of Sohlh2 and c‐kit

Ding

Zhang

et al. 2012

Molecular Reproduction Devel

View full text Add to dashboard Cite

Bone morphogenetic protein 4 (BMP4) is essential for the development of primordial follicles, although its underlying mechanism remains largely unknown. By using cultured ovaries, the effects of BMP4 and the potential signal transduction pathways were investigated. Ovaries from 3-day-old female mouse pups were maintained in organ culture in the absence (control) or presence of BMP4 (100 ng/ml). At different culture time, the effects of BMP4 on primordial follicle growth and survival were assayed by follicle count and TUNEL labeling. The expression of phospho-SMAD1/5/8, Sohlh2, and c-kit were measured by immunohistochemistry, RT-PCR, and Western blotting. Immunohistochemistry was also performed to determine the expression pattern of BMP4, pSMAD1/5/8, Sohlh2, and c-kit in vivo during ovarian development. The results showed treatments of ovaries with BMP4 resulted in a significant (P < 0.05) increase on the primordial-to-primary follicle transition. The oocytes of primordial follicles treated with BMP4 were also less likely to undergo apoptosis. BMP4 enhanced the phosphorylation of SMAD1/5/8 and up-regulated the expression of Sohlh2 and c-kit in primordial follicles. During ovarian development in vivo, Sohlh2, and c-kit exhibited similar expression patterns to BMP4 and pSMAD1/5/8 in primordial follicles. The present studies suggest that BMP4/SMAD signaling pathway initiate primordial follicle growth and prevented oocyte apoptosis via up-regulation of Sohlh2 and c-kit.

show abstract

Dynamic expression of bone morphogenetic protein 4 in reproductive organs of female mice

Cited by 27 publications

References 40 publications

The impact of passive immunisation against BMPRIB and BMP4 on follicle development and ovulation in mice

The impact of passive immunisation against BMPRIB and BMP4 on follicle development and ovulation in mice

Changes in granulosa cells' gene expression associated with increased oocyte competence in bovine

Effects of BMP4/SMAD signaling pathway on mouse primordial follicle growth and survival via up‐regulation of Sohlh2 and c‐kit

Contact Info

Product

Resources

About