Dynamic distribution of region-specific maternal protein during oogenesis and early embryogenesis of Xenopus laevis

Suzuki, Akio; Manabe, Junichi; Hirakawa, Asako

doi:10.1007/bf00361340

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…1 B, insert, arrows). It was reported that the radial striations were disorganized and that the antigen molecules were uniformly distributed all over the animal hemisphere of the mature oocyte in the oviduct (Suzuki et al 1991). In the present experiment, progesterone-treated oocytes underwent germinal vesicle breakdown in vitro after 6 h. During progesterone-induced maturation (Fig.…”

Section: Effects Of Germinal Vesicle Removal and K+-free Solutionmentioning

confidence: 62%

“…Production of the hybridoma, preparation of the monoclonal antibody (MoAb Xa5B6) and the procedures for immunohistochemical examination were described in ore previous paper (Suzuki et al 1991).…”

Section: Preparation Of Moab Za5b6 and Immunohistochemistrymentioning

confidence: 99%

“…We have previously reported a region-specific maternal protein which distributed more restrictively in the ectomesoderm region during early Xenopus embryogenesis (Suzuki et al 1991). The distribution of maternal protein molecules was shown to be completely different in the immature oocyte and the matured egg.…”

Section: Introductionmentioning

confidence: 97%

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Possible mechanisms in the rearrangement of non-yolk cytoplasmic materials during maturation of theXenopus laevis oocyte

Suzuki

Manabe

Imoh

1993

Roux's Arch Dev Biol

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Using the monoclonal antibody (MoAb) Xa5B6 as probe, the authors examined the mechanisms of cytoplasmic rearrangement occurring during maturation of theXenopus oocyte. The antigen molecules recognized by the MoAb are arranged in radial striations of the oocyte cytoplasm. The radial striations were disorganized in vitro by progesterone treatment, and the antigen molecules were uniformly distributed, predominantly in the animal hemisphere. Even when the germinal vesicle was mechanically removed or when germinal vesicle breakdown was suppressed in a K-free medium, progesterone induced a disorganization of the radial striations. This progesterone-induced disorganization was inhibited by the protein synthesis inhibitor cycloheximide. When full-sized oocytes were treated with cytochalasin B, the radial striations were also disorganized, but the antigen molecules did not disperse into the large mass. Colchicine treatment had little effect. Antigen molecules were no longer arranged in radial striations and were completely dispersed when the oocyte was simultaneously treated with both drugs. These results indicate that the two compartments in the oocyte cytoplasm, the yolk-free cytoplasm and yolk column, are organized by different types of cytoskeletal system. It is also suggested that the maturation-promoting factor (MPF) activated during progesterone-induced maturation disrupts these cytoskeletal systems and disorganizes the radial striations.

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Section: Effects Of Germinal Vesicle Removal and K+-free Solutionmentioning

confidence: 62%

Section: Preparation Of Moab Za5b6 and Immunohistochemistrymentioning

confidence: 99%

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Possible mechanisms in the rearrangement of non-yolk cytoplasmic materials during maturation of theXenopus laevis oocyte

Suzuki

Manabe

Imoh

1993

Roux's Arch Dev Biol

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“…Production of the hybridomas † , preparation of the monoclonal antibody, and the procedures for immunohistochemical analysis have been described in Suzuki et al (1991).…”

Section: Methodsmentioning

confidence: 99%

Study of Cynops pyrrhogaster notochord differentiation using a novel monoclonal antibody

et al. 2002

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Two monoclonal antibodies which reacted specifically with the notochord of the early Cynops pyrrhogaster embryo were screened. The antigen molecules were detected within and around the notochord. They were first found mostly between the neural plate and the dorsal part of the notochord in the early neurula (stage 15). They were subsequently detected between the notochord and the somite in the advanced embryo, and they were last detected between the notochord and the underlying endoderm. Whole-mount labeling indicated that the antigen molecules were first detected in the anterior half of the notochord in the early neurula (stage 15). The signals gradually spread along the anterior-posterior axis, especially towards the posterior region. This fact suggests that notochord differentiation progresses from the anterior region which first receives the dorsal mesoderm-inducing signals released horizontally from the lower dorsal marginal zone during early gastrulation. The present study suggested that: (i) notochord differentiation proceeds from the anterior region; and (ii) secretion of the antigen molecules results in the drawing of a boundary between the adjacent tissues.

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Localized Maternal Proteins inXenopusRevealed by Subtractive Immunization

Denegre

Ludwig

Mowry

1997

Developmental Biology

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It has long been appreciated that the localization of cytoplasmic determinants in the egg can provide the foundation for patterning in the embryo. Differences in cell fate among the early blastomeres are thus a consequence of asymmetric distributions of informational molecules prior to fertilization. The frog egg has a single axis of asymmetry present prior to fertilization, the animal/vegetal axis, and the localization of developmental information appears to be polarized along this axis. Such developmental information can be localized as either RNA or protein; localized RNAs are well documented in the Xenopus oocyte, and some are thought to play roles in axial patterning. While it is apparent that not all of the localized maternal components are RNAs, much less is known about maternal proteins that might be localized in the egg. In the present study, we have taken a novel approach to identify localized maternal proteins within the Xenopus egg. Using a subtractive immunization strategy, we have generated monoclonal antibodies which recognize antigens that are restricted to the vegetal cortex of fertilized eggs. Analysis of biogenesis during oogenesis reveals two distinct patterns of localization to the cortex. At least three of these localized antigens are proteins, and these localized proteins could represent maternal determinants with roles in patterning.

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Dynamic distribution of region-specific maternal protein during oogenesis and early embryogenesis of Xenopus laevis

Cited by 10 publications

References 20 publications

Possible mechanisms in the rearrangement of non-yolk cytoplasmic materials during maturation of theXenopus laevis oocyte

Possible mechanisms in the rearrangement of non-yolk cytoplasmic materials during maturation of theXenopus laevis oocyte

Study of Cynops pyrrhogaster notochord differentiation using a novel monoclonal antibody

Localized Maternal Proteins inXenopusRevealed by Subtractive Immunization

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