Dynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors

Stankovic, Nancy; Schloesser, Marie; Joris, Marine; Sauvage, Eric; Hanikenne, Marc; Motté, Patrick

doi:10.1104/pp.15.01338

Cited by 33 publications

(42 citation statements)

References 78 publications

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“…Furthermore, SR45 also co‐localized with OsFKBP20‐1b in cytoplasmic foci (Figure b). A recent study using leptomycin B and a fluorescence recovery after photobleaching assay showed that SR45 is a nucleocytoplasmic shuttling protein whose shuttling is mediated by the CRM1 pathway (Stankovic et al , ). Nevertheless, to date, the precise cytoplasmic localization of SR45 in plant cells has not been precisely determined because it is subject to rapid nucleocytoplasmic shuttling or is expressed at aberrant levels in transient assays.…”

Section: Discussionmentioning

confidence: 99%

“…Arabidopsis encodes one SR45 gene ( AtSR45 ) that produces seven different mature transcripts via alternative splicing, while the rice genome harbours two SR45 gene copies because of duplication (Ali et al , ; Narsai et al , ). In addition to U1 snRNP 70K, several other SR proteins, including SC34‐like (SR33), RSZ1, SR30, SR34, and spliceosomal components SKIP and CACTIN, interact with SR45 to regulate pre‐mRNA splicing (Golovkin and Reddy, ; Mayeda et al , ; Golovkin and Reddy, ; Carvalho et al , ; Wang et al , ; Baldwin et al , ; Stankovic et al , ). Recent studies have indicated that SR45 plays an essential role in plant growth and adaptation to environment stress (Carvalho et al , ; Xing et al , ; Carvalho and Szakonyi, ; Albaqami et al , ).…”

Section: Introductionmentioning

confidence: 99%

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OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

et al. 2020

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Sessile plants have evolved distinct mechanisms to respond and adapt to adverse environmental conditions through diverse mechanisms including RNA processing. While the role of RNA processing in the stress response is well understood for Arabidopsis thaliana, limited information is available for rice (Oryza sativa).Here, we show that OsFKBP20-1b, belonging to the immunophilin family, interacts with the splicing factor OsSR45 in both nuclear speckles and cytoplasmic foci, and plays an essential role in post-transcriptional regulation of abiotic stress response. The expression of OsFKBP20-1b was highly upregulated under various abiotic stresses. Moreover genetic analysis revealed that OsFKBP20-1b positively affected transcription and pre-mRNA splicing of stress-responsive genes under abiotic stress conditions. In osfkbp20-1b loss-of-function mutants, the expression of stress-responsive genes was downregulated, while that of their splicing variants was increased. Conversely, in plants overexpressing OsFKBP20-1b, the expression of the same stress-responsive genes was strikingly upregulated under abiotic stress. In vivo experiments demonstrated that OsFKBP20-1b directly maintains protein stability of OsSR45 splicing factor. Furthermore, we found that the plant-specific OsFKBP20-1b gene has uniquely evolved as a paralogue only in some Poaceae species. Together, our findings suggest that OsFKBP20-1b-mediated RNA processing contributes to stress adaptation in rice.OsFKBP20-1b is involved in pre-mRNA processing under abiotic stress 999 Subcellular localization and co-localizationThe full-length coding sequence (CDS) of OsFKBP20-1b was cloned into the pCAMBIA1302 binary vector, and the recombinant

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

et al. 2020

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“…Interestingly, Day et al (2012) have also reported an interaction between SR45 and the spliceosomal factor U2AF 35 , which in metazoans is involved in 39 splice site recognition (Wu et al, 1999;Wahl et al, 2009). Moreover, SR45 has been found to interact with three U5 small nuclear ribonucleoprotein proteins (Zhang et al, 2014) as well as with several other Arabidopsis SR proteins, namely, SCL33, RSZ21, SR30, SR34, and SR34a (Golovkin and Reddy, 1999;Zhang et al, 2014;Stankovic et al, 2016). Two other reported SR45-interacting proteins in Arabidopsis are the spliceosomal component SKIP (Wang et al, 2012), which confers salt stress tolerance (Feng et al, 2015), and CACTIN, an essential nuclear factor required for embryogenesis (Baldwin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Early studies had shown that AFC2, a LAMMER-type protein kinase, is able to interact with and phosphorylate SR45 in vitro (Golovkin and Reddy, 1999) and found SR45 to be confined to the nucleus, either diffusely distributed in the nucleoplasm or concentrated in speckles, with its subnuclear dynamics being regulated by phosphorylation, ATP, and transcription (Ali et al, 2003;Ali and Reddy, 2006). However, recently reported evidence substantiates nucleocytoplasmic shuttling activity for SR45, with possible involvement of the nuclear exporter protein XPO1 (Stankovic et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis SR45 Splicing Factor, a Negative Regulator of Sugar Signaling, Modulates SNF1-Related Protein Kinase 1 Stability

et al. 2016

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The ability to sense and respond to sugar signals allows plants to cope with environmental and metabolic changes by adjusting growth and development accordingly. We previously reported that the SR45 splicing factor negatively regulates glucose signaling during early seedling development in Arabidopsis thaliana. Here, we show that under glucose-fed conditions, the Arabidopsis sr45-1 loss-of-function mutant contains higher amounts of the energy-sensing SNF1-Related Protein Kinase 1 (SnRK1) despite unaffected SnRK1 transcript levels. In agreement, marker genes for SnRK1 activity are upregulated in sr45-1 plants, and the glucose hypersensitivity of sr45-1 is attenuated by disruption of the SnRK1 gene. Using a high-resolution RT-PCR panel, we found that the sr45-1 mutation broadly targets alternative splicing in vivo, including that of the SR45 pre-mRNA itself. Importantly, the enhanced SnRK1 levels in sr45-1 are suppressed by a proteasome inhibitor, indicating that SR45 promotes targeting of the SnRK1 protein for proteasomal destruction. Finally, we demonstrate that SR45 regulates alternative splicing of the Arabidopsis 5PTase13 gene, which encodes an inositol polyphosphate 5-phosphatase previously shown to interact with and regulate the stability of SnRK1 in vitro, thus providing a mechanistic link between SR45 function and the modulation of degradation of the SnRK1 energy sensor in response to sugars.

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“…For example, serine and arginine-rich protein 45 (SR45), a component of the spliceosome that interacts with three U5 small nuclear ribonucleo proteins (snRNPs) as well as with several other SR proteins (e.g. SCL33, RSZ21, SR30, SR34 and SR34a), is required for genome-wide pre-mRNA splicing in Arabidopsis (Golovkin & Reddy, 1999;Xing et al, 2015;Stankovic et al, 2016). However, SR45 is also a component of the apoptosis-and splicing-associated protein (ASAP) complex, which is involved in the silencing of FLC expression by Polycomb during vernalization in Arabidopsis (Q€ uesta et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

SKIP regulates environmental fitness and floral transition by forming two distinct complexes in Arabidopsis

Yang

Shang

et al. 2019

New Phytologist

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Summary Ski‐interacting protein (SKIP) is a bifunctional regulator of gene expression that works as a splicing factor as part of the spliceosome and as a transcriptional activator by interacting with EARLY FLOWERING 7 (ELF7). MOS4‐Associated Complex 3A (MAC3A) and MAC3B interact physically and genetically with SKIP, mediate the alternative splicing of c. 50% of the expressed genes in the Arabidopsis genome, and are required for the splicing of a similar set of genes to that of SKIP. SKIP interacts physically and genetically with splicing factors and Polymerase‐Associated Factor 1 complex (Paf1c) components. However, these splicing factors do not interact either physically or genetically with Paf1c components. The SKIP‐spliceosome complex mediates circadian clock function and abiotic stress responses by controlling the alternative splicing of pre‐mRNAs encoded by clock‐ and stress tolerance‐related genes. The SKIP‐Paf1c complex regulates the floral transition by activating FLOWERING LOCUS C (FLC) transcription. Our data reveal that SKIP regulates floral transition and environmental fitness via its incorporation into two distinct complexes that regulate gene expression transcriptionally and post‐transcriptionally, respectively. It will be interesting to discover in future studies whether SKIP is required for integration of environmental fitness and growth by control of the incorporation of SKIP into spliceosome or Paf1c in plants.

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Dynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors

Cited by 33 publications

References 78 publications

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

The Arabidopsis SR45 Splicing Factor, a Negative Regulator of Sugar Signaling, Modulates SNF1-Related Protein Kinase 1 Stability

SKIP regulates environmental fitness and floral transition by forming two distinct complexes in Arabidopsis

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