Dynamic compressive loading of image-guided tissue engineered meniscal constructs

Ballyns, Jeffrey J.; Bonassar, Lawrence J.

doi:10.1016/j.jbiomech.2010.09.017

Cited by 68 publications

(47 citation statements)

References 40 publications

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“…Further, previously described ABS loading trays (Ballyns and Bonassar, 2011) were redesigned to have a trough at the base of the meniscus that extensions lay in. Stainless steel clamps infused with bronze (Shapeways, NY) were screwed down over the extensions to secure them throughout culture (Fig.…”

Section: Application Of Boundary Conditionsmentioning

confidence: 99%

Induction of fiber alignment and mechanical anisotropy in tissue engineered menisci with mechanical anchoring

Puetzer

Koo

Bonassar

2015

Journal of Biomechanics

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131

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This study investigated the effect of mechanical anchoring on the development of fiber organization and anisotropy in anatomically shaped tissue engineered menisci. Bovine meniscal fibrochondrocytes were mixed with collagen and injected into molds designed to produce meniscus implants with 12 mm extensions at each horn. After a day of static culture, 10 and 20mg/ml collagen menisci were either clamped or unclamped and cultured for up to 8 weeks. Clamped menisci were anchored in culture trays throughout culture to mimic the native meniscus horn attachment sites, restrict contraction circumferentially, and encourage circumferential alignment. Clamped menisci retained their size and shape, and by 8 weeks developed circumferential and radial fiber organization that resembled native meniscus. Clamping also increased collagen accumulation and improved mechanical properties compared to unclamped menisci. Enhanced organization in clamped menisci was further reflected in the development of anisotropic tensile properties, with 2-3 fold higher circumferential moduli compared to radial moduli, a similar ratio to native meniscus. Ten and 20mg/ml clamped menisci had similar levels of organization, with 20mg/ml menisci producing larger diameter fibers and significantly better mechanical properties. Collectively, these data demonstrate the benefit of using bio-inspired mechanical boundary conditions to drive the formation of a highly organized collagen fiber network.

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Section: Application Of Boundary Conditionsmentioning

confidence: 99%

Induction of fiber alignment and mechanical anisotropy in tissue engineered menisci with mechanical anchoring

Puetzer

Koo

Bonassar

2015

Journal of Biomechanics

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131

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“…These results were consistent with a number of other studies that have utilized chondrocytes encapsulated within alginate for the formation of three-dimensional molded cartilage constructs. [36][37][38] In the present study, the formation of collagen and elastin were observed to be localized to the periphery of the construct, suggesting that there may be limitations in mass transport within these large-scale constructs. However, GAG formation was observed throughout the construct thickness, which suggests that GAG formation may occur more rapidly than does collagen and elastin formation.…”

Section: Discussionmentioning

confidence: 51%

Computed Tomography-Guided Tissue Engineering of Upper Airway Cartilage

Brown

Siebenlist²,

Cheetham³

et al. 2014

Tissue Engineering Part C: Methods

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Normal laryngeal function has a large impact on quality of life, and dysfunction can be life threatening. In general, airway obstructions arise from a reduction in neuromuscular function or a decrease in mechanical stiffness of the structures of the upper airway. These reductions decrease the ability of the airway to resist inspiratory or expiratory pressures, causing laryngeal collapse. We propose to restore airway patency through methods that replace damaged tissue and improve the stiffness of airway structures. A number of recent studies have utilized image-guided approaches to create cell-seeded constructs that reproduce the shape and size of the tissue of interest with high geometric fidelity. The objective of the present study was to establish a tissue engineering approach to the creation of viable constructs that approximate the shape and size of equine airway structures, in particular the epiglottis. Computed tomography images were used to create three-dimensional computer models of the cartilaginous structures of the larynx. Anatomically shaped injection molds were created from the three-dimensional models and were seeded with bovine auricular chondrocytes that were suspended within alginate before static culture. Constructs were then cultured for approximately 4 weeks post-seeding and evaluated for biochemical content, biomechanical properties, and histologic architecture. Results showed that the three-dimensional molded constructs had the approximate size and shape of the equine epiglottis and that it is possible to seed such constructs while maintaining 75%+ cell viability. Extracellular matrix content was observed to increase with time in culture and was accompanied by an increase in the mechanical stiffness of the construct. If successful, such an approach may represent a significant improvement on the currently available treatments for damaged airway cartilage and may provide clinical options for replacement of damaged tissue during treatment of obstructive airway disease.

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“…To address this problem, mechanical stimuli, such as static loading, dynamic compression, or hydrostatic pressure, have often been used to increase chondrocyte collagen production. 10,43,44 Although the mechanism of chondrocyte mechanotransduction is not fully understood, previous studies suggest that ATP signaling, ion channels, and the primary cilia are likely involved in transducing extracellular biomechanical signals to both intracellular chemical and electrical signals. 28,45,46 Given calcium's role in the mechanotransduction pathway, the application of Ca 2 + -modulating agents to developing neocartilage presents as a potential strategy to improve the ECM of neotissues and enhance their functional properties.…”

Section: Discussionmentioning

confidence: 99%

Digoxin and Adenosine Triphosphate Enhance the Functional Properties of Tissue-Engineered Cartilage

Makris

Huang

et al. 2015

Tissue Engineering Part A

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Toward developing engineered cartilage for the treatment of cartilage defects, achieving relevant functional properties before implantation remains a significant challenge. Various chemical and mechanical stimuli have been used to enhance the functional properties of engineered musculoskeletal tissues. Recently, Ca 2 + -modulating agents have been used to enhance matrix synthesis and biomechanical properties of engineered cartilage. The objective of this study was to determine whether other known Ca 2 + modulators, digoxin and adenosine triphosphate (ATP), can be employed as novel stimuli to increase collagen synthesis and functional properties of engineered cartilage. Neocartilage constructs were formed by scaffold-free self-assembling of primary bovine articular chondrocytes. Digoxin, ATP, or both agents were added to the culture medium for 1 h/ day on days 10-14. After 4 weeks of culture, neocartilage properties were assessed for gross morphology, biochemical composition, and biomechanical properties. Digoxin and ATP were found to increase neocartilage collagen content by 52-110% over untreated controls, while maintaining proteoglycan content near native tissue values. Furthermore, digoxin and ATP increased the tensile modulus by 280% and 180%, respectively, while the application of both agents increased the modulus by 380%. The trends in tensile properties were found to correlate with the amount of collagen cross-linking. Live Ca 2 + imaging experiments revealed that both digoxin and ATP were able to increase Ca 2 + oscillations in monolayer-cultured chondrocytes. This study provides a novel approach toward directing neocartilage maturation and enhancing its functional properties using novel Ca 2 + modulators.

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Dynamic compressive loading of image-guided tissue engineered meniscal constructs

Cited by 68 publications

References 40 publications

Induction of fiber alignment and mechanical anisotropy in tissue engineered menisci with mechanical anchoring

Induction of fiber alignment and mechanical anisotropy in tissue engineered menisci with mechanical anchoring

Computed Tomography-Guided Tissue Engineering of Upper Airway Cartilage

Digoxin and Adenosine Triphosphate Enhance the Functional Properties of Tissue-Engineered Cartilage

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