Dynamic complexity: plant receptor complexes at the plasma membrane

Burkart, Rebecca C.; Stahl, Yvonne

doi:10.1016/j.pbi.2017.06.016

Cited by 39 publications

(42 citation statements)

References 55 publications

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“…Physical recruitment of membrane-resident ligand-binding receptors into membrane nanodomains emerges as a common phenomenon within the plant kingdom (1,2). It represents a possible generic theme for the activation and maintenance of receptorcontrolled signal transduction.…”

Section: Introductionmentioning

confidence: 99%

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Symbiotic root infections inMedicago truncatularequire remorin-mediated receptor stabilization in membrane nanodomains

Liang

Stratil

Popp

et al. 2017

Preprint

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Plant cell infection is tightly controlled by cell surface receptor-like kinases (RLKs)Alike other RLKs the Medicago truncatula entry receptor LYK3 laterally segregates into membrane nanodomains in a stimulus-dependent manner. Although nanodomain localization arises as a generic feature of plant membrane proteins, molecular mechanisms underlying such dynamic transitions and their functional relevance remained poorly understood. Here, we demonstrate that actin and the flotillin protein FLOT4 form the primary and indispensable core of a specific nanodomain. Infection-dependent induction of the remorin protein and secondary molecular scaffold SYMREM1 results in subsequent recruitment of ligand-activated LYK3 and its stabilization within these membrane subcompartments. Reciprocally, the majority of this LYK3 receptor pool is destabilized at the plasma membrane and undergoes rapid endocytosis in symrem1 mutants upon rhizobial inoculation resulting in premature abortion of host cell infections.These data reveal that receptor recruitment into nanodomains is indispensable for their function during host cell infection.All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/179036 doi: bioRxiv preprint first posted online Dec. 31, 2017; 3 SIGNIFICANCE STATEMENT:Pattern recognition receptors control the cellular entry of pathogenic as well as symbiotic microbes. While ligand-induced changes in receptor mobility at the plasma membrane and their localization in membrane nanodomains appears as a general feature, the molecular mechanism and the biological relevance of this phenomenon remained unknown. Here, we show that immobilization of the symbiotic cell entry receptor LYK3 in nanodomains requires the presence of actin and the two molecular scaffold proteins FLOT4 and SYMREM1. While FLOT4 forms the initial core structure, infection-induced expression and subsequent physical interaction of SYMREM1 with LYK3 stabilizes the activated receptors in membrane nanodomains. This recruitment prevents its stimulusdependent endocytosis and ensures progression of the primary infection thread into root cortical cells.All rights reserved. No reuse allowed without permission.

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Section: Introductionmentioning

confidence: 99%

“…It represents a possible generic theme for the activation and maintenance of receptorcontrolled signal transduction. Indeed, a number of plant receptor-like kinases (RLKs), as well as receptor-associated and immunity-related proteins localize to nanodomains (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Symbiotic root infections inMedicago truncatularequire remorin-mediated receptor stabilization in membrane nanodomains

Liang

Stratil

Popp

et al. 2017

Preprint

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show abstract

“…These data reveal that receptor recruitment into nanodomains is indispensable for their function during host cell infection. membrane nanodomain | symbiosis | scaffold proteins | receptor | remorin P hysical recruitment of membrane-resident ligand-binding receptors into membrane nanodomains is as a common phenomenon within the plant kingdom (1,2). It represents a possible generic theme for the activation and maintenance of receptor-controlled signal transduction.…”

mentioning

confidence: 99%

“…It represents a possible generic theme for the activation and maintenance of receptor-controlled signal transduction. Indeed, a number of plant receptor-like kinases (RLKs), as well as receptor-associated and immunity-related proteins, localize to nanodomains (1)(2)(3). Given the diversity of coexisting nanodomains (4), these membrane compartments might serve as active hubs that allow a spatially confined assembly of pathway-specific and functionally related signaling complexes (3).…”

mentioning

confidence: 99%

Symbiotic root infections in Medicago truncatula require remorin-mediated receptor stabilization in membrane nanodomains

Liang

Stratil

Popp

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Plant cell infection is tightly controlled by cell surface receptor-like kinases (RLKs). Like other RLKs, the entry receptor LYK3 laterally segregates into membrane nanodomains in a stimulus-dependent manner. Although nanodomain localization arises as a generic feature of plant membrane proteins, the molecular mechanisms underlying such dynamic transitions and their functional relevance have remained poorly understood. Here we demonstrate that actin and the flotillin protein FLOT4 form the primary and indispensable core of a specific nanodomain. Infection-dependent induction of the remorin protein and secondary molecular scaffold SYMREM1 results in subsequent recruitment of ligand-activated LYK3 and its stabilization within these membrane subcompartments. Reciprocally, the majority of this LYK3 receptor pool is destabilized at the plasma membrane and undergoes rapid endocytosis in mutants on rhizobial inoculation, resulting in premature abortion of host cell infections. These data reveal that receptor recruitment into nanodomains is indispensable for their function during host cell infection.

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“…At low temperature (4 • C), the Lo phase structures of rafts can be recovered from membrane fractions as a consequence of the tight packing of the lipids and insolubility to non-ionic detergent (Triton X100), and are therefore referred to as 'detergent-insoluble membranes' (DIMs) [61,65]. Functionally, membrane rafts play regulatory roles in signal transduction, endocytosis and exocytosis, cell adhesion, actin cytoskeleton organisation and cell trafficking [59,[64][65][66].…”

Section: Membrane Raft Domains In the Flg22-mediated Defence Responsementioning

confidence: 99%

Prospects of Gene Knockouts in the Functional Study of MAMP-Triggered Immunity: A Review

Offor

Dubery

Piater

2020

IJMS

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Plants depend on both preformed and inducible defence responses to defend themselves against biotic stresses stemming from pathogen attacks. In this regard, plants perceive pathogenic threats from the environment through pattern recognition receptors (PRRs) that recognise microbe-associated molecular patterns (MAMPs), and so induce plant defence responses against invading pathogens. Close to thirty PRR proteins have been identified in plants, however, the molecular mechanisms underlying MAMP perception by these receptors/receptor complexes are not fully understood. As such, knockout (KO) of genes that code for PRRs and co-receptors/defence-associated proteins is a valuable tool to study plant immunity. The loss of gene activity often causes changes in the phenotype of the model plant, allowing in vivo studies of gene function and associated biological mechanisms. Here, we review the functions of selected PRRs, brassinosteroid insensitive 1 (BRI1) associated receptor kinase 1 (BAK1) and other associated defence proteins that have been identified in plants, and also outline KO lines generated by T-DNA insertional mutagenesis as well as the effect on MAMP perception—and triggered immunity (MTI). In addition, we further review the role of membrane raft domains in flg22-induced MTI in Arabidopsis, due to the vital role in the activation of several proteins that are part of the membrane raft domain theory in this regard.

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Dynamic complexity: plant receptor complexes at the plasma membrane

Cited by 39 publications

References 55 publications

Symbiotic root infections inMedicago truncatularequire remorin-mediated receptor stabilization in membrane nanodomains

Symbiotic root infections inMedicago truncatularequire remorin-mediated receptor stabilization in membrane nanodomains

Symbiotic root infections in Medicago truncatula require remorin-mediated receptor stabilization in membrane nanodomains

Prospects of Gene Knockouts in the Functional Study of MAMP-Triggered Immunity: A Review

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