Dynamic Combinatorial Libraries

Komáromy, Dávid; Nowak, Piotr; Otto, Sijbren

doi:10.1002/9781119075738.ch2

Cited by 6 publications

(2 citation statements)

References 406 publications

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“…Important advances in understanding chemical evolution have come from systems chemistry [24][25][26][27][28][29][30][31][32][33][34] , dynamic combinatorial chemistry (DCC) 35,36 , and models of self-organization 37 .…”

Section: Introductionmentioning

confidence: 99%

Evolution of Complex Chemical Mixtures Reveals Combinatorial Compression and Population Synchronicity

Matange,

Rajaei,

Capera-Aragonès

et al. 2023

Preprint

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Some of the most interesting open questions about the origins of life and molecular sciences center on chemical evolution and the spontaneous generation of complex and functional chemical species. The processes that generated the spectacular biopolymers that underlay biology demonstrate an untapped, by modern science, creative potential. We have established a robust, facile, and generally applicable platform for observing and analyzing chemical evolution using complex mixtures. While previous studies have characterized the formation of proto-polymers via chemical reactions, we systematically studied the process itself. We report empirical outcomes that were not foreseen or predicted. We have constructed an experimental platform to study the evolution of chemical systems that: (i) undergoes continuous recursive change with transitions to new chemical spaces while not converging throughout the course of the experiment, (ii) demonstrates chemical selection, during which combinatorial explosion is avoided, (iii) maintains synchronicity of molecular sub-populations, and (iv) harvests environmental energy that is stored in chemical energy. We have established some general guidelines for conducting chemical evolution. Our results suggest that chemical evolution can be adapted to produce a broad array of molecules with novel structures and functions.

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Section: Introductionmentioning

confidence: 99%

Evolution of Complex Chemical Mixtures Reveals Combinatorial Compression and Population Synchronicity

Matange,

Rajaei,

Capera-Aragonès

et al. 2023

Preprint

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“…A variety of template effects based on inorganic anions, organic molecules or external physical stimuli have been reported. 20 DCLs have been studied in multiple solvents systems, 21,22 at nanosystem interfaces, 23,24 and in response to changes in solvent environment. 25,26 Here we report the behaviour of a DCL in the multiphase environment provided by a membrane lipid bilayer in an aqueous phase, which opens the possibility for spatial and temporal control of the properties of the mixture.…”

Section: Introductionmentioning

confidence: 99%

Template effects of vesicles in dynamic covalent chemistry

Bravin

Hunter

2020

Chem. Sci.

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Templated Self‐Replication in Biomimetic Systems

et al. 2019

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nanostructures and nanopatterns. [13][14][15] The specificity of the molecular recognitions between replicative units is concomitant with the encoding and transmission of information, with the degree of specificity determining the fidelty and capacity of information transfer against competing background interactions in noisy "chemical" environments. [16] However, the ability to replicate in this manner does not imply the ability to transmit additional heritable information-simple replicators can only replicate information related to recognition and template directed autocatalysis as this information is intrinsically linked to phenotype. In contrast to most artificial autocatalytic replicators, living systems (including viruses) store the key instructions for replication on an inheritable genetic system rather than in the native structures of the individual sub-components. This decoupling of phenotype and genotype enables heredity and open-ended evolution, the possibility of an indefinite increase in complexity, as evolutionary innovations aquired by natural selection are anchored in the genotype only. [17,18] The most prominent self-replicating molecules are nucleic acids, which form the fundamental basis for information storage and propagation in biological systems. The ability of polynucleic acids to store information is self-evident, and based upon the ability to form cognate Watson-Crick base pair interactions. Nucleic acid systems may have also formed the first genetic replicators in biology. The RNA world hypothesis postulates a stage in the origin of life in which RNA proliferated before the emergence of DNA and proteins. To do this, RNA must be able to carry out several key roles: information storage, catalysis, and (self-) replication. Multiple examples of catalytic RNAs (ribozymes) are known both from nature and in vitro selection experiments, [19][20][21][22] but there is no fossil evidence of an RNA capable of (self-)replication without the involvement of proteins.While minimal nucleic acid-only systems with replicationlike properties have been identified by directed evolution and engineering, limitations such as low activity, lack of generality, and poor information capacity limit their usefulness in bottomup synthetic biology, except in origin of life research. For the creation of more complex biological in vitro systems, RNA or DNA replication mechanisms involving more powerful protein enzymes are likely to be necessary. Even so, the achievement of self-sustained in vitro self-replication inspired by the central dogma of molecular biology is currently hampered by the requirement to encode and efficiently express the enormous A key characteristic of living systems is the storage and replication of information, and as such the development of self-replicating systems capable of heredity is of great importance to the fields of synthetic biology and origin of life research. In this review, the design and implementation of self-replicating systems in the context of bottom-up synthetic biology is discusse...

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Dynamic Combinatorial Libraries

Cited by 6 publications

References 406 publications

Evolution of Complex Chemical Mixtures Reveals Combinatorial Compression and Population Synchronicity

Evolution of Complex Chemical Mixtures Reveals Combinatorial Compression and Population Synchronicity

Template effects of vesicles in dynamic covalent chemistry

Templated Self‐Replication in Biomimetic Systems

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