Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits

Jin-gan, Liu; Guo, Sheng; He, Hongju; Zhang, Haiying; Gong, Guoyi; Ren, Yi; Xu, Yong

doi:10.1007/s11738-013-1356-0

Cited by 48 publications

(54 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our previous study indicated that the enzyme activity of insoluble acid invertase and sucrose content were highly correlated in the fruit flesh tissue of 97103 and PI296341-FR [1]. A total of five insoluble acid invertase genes were found in the watermelon genome.…”

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development

Guo

Sun

Zhang³

et al. 2015

PLoS ONE

Self Cite

124

View full text Add to dashboard Cite

Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an important vegetable crop world-wide. Watermelon fruit quality is a complex trait determined by various factors such as sugar content, flesh color and flesh texture. Fruit quality and developmental process of cultivated and wild watermelon are highly different. To systematically understand the molecular basis of these differences, we compared transcriptome profiles of fruit tissues of cultivated watermelon 97103 and wild watermelon PI296341-FR. We identified 2,452, 826 and 322 differentially expressed genes in cultivated flesh, cultivated mesocarp and wild flesh, respectively, during fruit development. Gene ontology enrichment analysis of these genes indicated that biological processes and metabolic pathways related to fruit quality such as sweetness and flavor were significantly changed only in the flesh of 97103 during fruit development, while those related to abiotic stress response were changed mainly in the flesh of PI296341-FR. Our comparative transcriptome profiling analysis identified critical genes potentially involved in controlling fruit quality traits including α-galactosidase, invertase, UDP-galactose/glucose pyrophosphorylase and sugar transporter genes involved in the determination of fruit sugar content, phytoene synthase, β-carotene hydroxylase, 9-cis-epoxycarotenoid dioxygenase and carotenoid cleavage dioxygenase genes involved in carotenoid metabolism, and 4-coumarate:coenzyme A ligase, cellulose synthase, pectinesterase, pectinesterase inhibitor, polygalacturonase inhibitor and α-mannosidase genes involved in the regulation of flesh texture. In addition, we found that genes in the ethylene biosynthesis and signaling pathway including ACC oxidase, ethylene receptor and ethylene responsive factor showed highly ripening-associated expression patterns, indicating a possible role of ethylene in fruit development and ripening of watermelon, a non-climacteric fruit. Our analysis provides novel insights into watermelon fruit quality and ripening biology. Furthermore, the comparative expression profile data we developed provides a valuable resource to accelerate functional studies in watermelon and facilitate watermelon crop improvement.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development

Guo

Sun

Zhang³

et al. 2015

PLoS ONE

Self Cite

124

View full text Add to dashboard Cite

show abstract

“…Sugars are crucial components of cultivated watermelon fruit quality [49] and serve as imperative signals in the regulation of fruit ripening [19, 50]. As with all other fruits of cucurbits, watermelon sugars consist of sucrose, fructose and glucose [51].…”

Section: Discussionmentioning

confidence: 99%

“…The sweetness level of watermelon is determined by calculating the total sugar contents and by determining the composition of glucose, fructose and sucrose [52]. At the early stage of ripening, the sucrose content in watermelon is very low but increases at later stages, whereas the fructose and glucose contents remain constant [49, 51]. In this study, the TSS, fructose and sucrose contents were found to be very low at the early stage of fruit development and increased rapidly at later stages in COS and LSW177, whereas the glucose content peaked in the early stage and then decreased to its initial level during fruit ripening.…”

Section: Discussionmentioning

confidence: 99%

“…Stachyose, raffinose and sucrose are the main sugars transported from the leaves to the fruit in the phloem of cucurbit plants [53]. Furthermore, a considerable amount of translocated sugars undergoes phloem loading, distribution and metabolism, which are controlled by pivotal sugar metabolism enzymes [49]. In this study, more differentially expressed genes were significantly enriched in pathways related to the biosynthesis/catabolism of stachyose, raffinose and sucrose, such as ‘galactose metabolism’, ‘pentose and glucoronate interconversions’, ‘other glycan degradation’, ‘fructose and mannose metabolism’, ‘glycolysis/gluconeogenesis’, and ‘starch and sucrose metabolism’.…”

Section: Discussionmentioning

confidence: 99%

“…These domino effects indicated that Cla007286 might function as an essential element for phloem unloading and sink strength evaluation during development. Previous studies have suggested that the sugars in watermelon are inspected primarily by three enzyme families: SuSys, SPSs and invertases [49, 51]. SuSy is a focal enzyme that can catalyze both the formation and hydrolysis of sucrose in plants.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

et al. 2017

View full text Add to dashboard Cite

BackgroundWatermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process.ResultsIn this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177). We first identified 797 novel genes to extend the available reference gene set. Second, 3958 genes in COS and 3503 genes in LSW177 showed at least two-fold variation in expression, and a large number of these differentially expressed genes (DEGs) during fruit ripening were related to carotenoid biosynthesis, plant hormone pathways, and sugar and cell wall metabolism. Third, we noted a correlation between ripening-associated transcripts and metabolites and the key function of these metabolic pathways during fruit ripening.ConclusionThe results revealed several ripening-associated actions and provide novel insights into the molecular mechanisms underlying the regulation of watermelon fruit ripening.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3442-3) contains supplementary material, which is available to authorized users.

show abstract

Challenges of Traditional Breeding in Watermelon

Choudhary,

Padmanabha,

Jat

et al. 2023

Compendium of Plant Genomes

View full text Add to dashboard Cite

Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits

Cited by 48 publications

References 42 publications

Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development

Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development

Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

Challenges of Traditional Breeding in Watermelon

Contact Info

Product

Resources

About