Dynamic Changes of Urine Proteome in Rat Models Inoculated with Two Different Hepatoma Cell Lines

Abstract

Urine can accumulate systemic changes with no mechanism to be stable, which may reflect early changes associated with physiological or pathophysiological processes. To explore the potential value of the urine proteome, two rat models were established by intrahepatic injection of two different hepatoma cell lines, CBRH-7919 and RH-35. Urine samples were collected and analyzed. Compared with controls, the two models exhibited different numbers and types of differentially expressed urinary proteins despite having similar histological results. The results were compared with the urine proteome of a Walker 256 (W-256) liver tumor model. The differentially expressed urinary protein patterns in the three models were different. These findings demonstrate that changes in the urine proteomes of the two models can be detected at early stages and that the patterns of differentially expressed urinary proteins can differ even when the histological results are similar. Urinary proteins have potential utility for distinguishing among different tumor cells grown in the same organ.

Changes in the urinary proteome in rats with regular swimming exercise

“…Recent studies have shown that the urine proteome has potential for differential diagnosis. For example, early urinary proteins were different when the same tumor cells were grown in different organs ( Wu, Guo & Gao, 2017 ; Wei et al, 2019 ; Zhang et al, 2019 ; Wang et al, 2020 ; Zhang, Gao & Gao, 2020 ) and when different cells were injected into the same organ ( Zhang et al, 2018a ; Zhang et al, 2018b ; Zhang et al, 2021 ). Furthermore, several clinical studies performed urine proteomics to discover diagnostic biomarkers, such as for gastric cancer ( Shimura et al, 2020 ) and familial Parkinson’s disease ( Winter et al, 2021 ).…”

Changes in the urinary proteome in rats with regular swimming exercise

“…Recent studies have also shown that the urine proteome has potential for differential diagnosis. For example, early urinary proteins were different when the same tumor cells were grown in different organs [6, 11–14] and when different cells were injected into the same organ [12, 15]. Furthermore, several clinical studies performed urine proteomics to discover diagnostic biomarkers, such as for gastric cancer [16] and familial Parkinson’s disease [17].…”