Dynamic Changes of Plasma Metabolome in Response to Severe Feed Restriction in Pregnant Ewes

Abstract

Maternal metabolic disorders in ewes induced by energy deficiency have a detrimental effect on the maternal health and lambs. However, the dynamic processes of metabolic disorders are unknown. Therefore, this study attempted to explore the dynamic changes of maternal metabolism based on metabolomics approach during energy deficiency in pregnant ewes. Twenty pregnant Hu sheep were fed a basic diet or a 70% restricted basic diet. The HPLC-MS platform was applied to identify blood metabolites. Principal component analysis of blood samples based on their metabolic profile showed that blood samples of feed restriction group differed after the treatment. In particular, when comparing both groups, there were 120, 129, and 114 differential metabolites at day 5, day 10, and day 114 between the two groups, respectively. Enrichment analysis results showed that four metabolic pathways (glycerophospholipid metabolism, linoleic acid metabolism, arginine and proline metabolism, and aminoacyl-tRNA biosynthesis) at day 5, four metabolic pathways (aminoacyl-tRNA biosynthesis, aminoacyl-tRNA biosynthesis, glycerophospholipid metabolism, and citrate cycle) at day 10, and nine metabolic pathways (aminoacyl-tRNA biosynthesis, synthesis and degradation of ketone bodies, glycerophospholipid metabolism, butanoate metabolism, linoleic acid metabolism, citrate cycle, alanine, aspartate and glutamate metabolism, valine, leucine and isoleucine biosynthesis, and arginine and proline metabolism) at day 15 were significantly enriched between the two groups. These findings revealed temporal changes of metabolic disorders in pregnant ewes caused by severe feed restriction, which may provide insights into mitigation measures.

Transcriptome analysis reveals liver metabolism programming in kids from nutritional restricted goats during mid-gestation

“…These results suggested that maternal undernutrition may lead to programmed hepatic metabolism and the programming effect didn’t observe in a short time (fetal phase) but appeared after a long time (kid phase). Guo et al (2019) observed that citrate cycle was significantly suppressed by severe feed restriction in sheep with lower level of glucose-6-phosphate, citrate and -ketoglutarate in the blood sample. Milk replacer restriction downregulated the expression of isocitrate dehydrogenase 2 ( IDH2 ) and oxoglutarate dehydrogenase complex (OGDH) which involved in citrate cycle of lambs ( Santos et al, 2018b ).…”

“…These results suggested that maternal undernutrition may lead to programmed hepatic metabolism and the programming effect didn't observe in a short time (fetal phase) but appeared after a long time (kid phase). Guo et al (2019) et al, 2018b). These results were similar to the results of our study that feed restriction downregulated citrate cycle on transcriptome with lower expression of succinate dehydrogenase complex subunit C (SDHC) and IDH1.…”

Metabolomics analysis of the serum from children with urolithiasis using UPLC‐MS

“…The calculated p value was established based on pathway enrichment analysis, and the pathway impact value was based on pathway topology analysis. The most relevant metabolic pathways were the retinol metabolic pathway, porphyrin and chlorophyll metabolic pathway, and steroid hormone biosynthesis pathway | 9 PEDIATRIC UROLITHIASIS SERUM METABOLOMICS F I G U R E 5 Metabolic pathways and metabolites associated with pediatric urolithiasis. (a) Schematic of abnormal metabolic pathways in patients with urolithiasis.…”

“…Data acquisition was performed according to the prior study. 9 The 10 μl of the supernatant were injected into the LC‐MS (Ultimate 3000 LC, Q Exactive; Thermo Scientific) in random order. A reverse‐phase 2.1*100 mm 1.9 μm C18 column (Hyper gold) was used for separation.…”

Metabolomic Profiling Reveals the Difference on Reproductive Performance between High and Low Lactational Weight Loss Sows

“…Lipids, as physiological fuels, are stored in adipose tissue with low water content and high energy density [45]. Humans and animals usually break down fat to meet their energy requirements when the energy supply is restricted.…”