Dynamic changes in α- and β-amylase activities and gene expression in bulbs of the Oriental hybrid lily ‘Siberia’ during dormancy release

Chun-qi, Yang; Li, Qiuhua; Jiang, Xinqiang; Fan, Youwei; Gao, Junping; Zhang, Changqing

doi:10.1080/14620316.2015.11668742

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Meanwhile, soluble sugars (glucose, sucrose, fructose, trehalose, etc.) increase during the transition from endodormancy to ecodormancy in buds of species such as leafy spurge, lily, sweet cherry and tree peony [26,65,[69][70][71]. At the bud dormancy induction stage, sucrose in parenchyma cells declines and Tre6P (Trehalose 6-phosphate) decreases, which activates SnRK1 activity and stimulates ABA biosynthesis [72].…”

Section: Cross-talk Between Aba and Sugars In Bud Dormancymentioning

confidence: 99%

ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective

Pan

Liang

Sui

et al. 2021

Genes

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Bud dormancy is an evolved trait that confers adaptation to harsh environments, and affects flower differentiation, crop yield and vegetative growth in perennials. ABA is a stress hormone and a major regulator of dormancy. Although the physiology of bud dormancy is complex, several advancements have been achieved in this field recently by using genetics, omics and bioinformatics methods. Here, we review the current knowledge on the role of ABA and environmental signals, as well as the interplay of other hormones and sucrose, in the regulation of this process. We also discuss emerging potential mechanisms in this physiological process, including epigenetic regulation.

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Section: Cross-talk Between Aba and Sugars In Bud Dormancymentioning

confidence: 99%

ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective

Pan

Liang

Sui

et al. 2021

Genes

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“…It was indicated that during the lily vernalization period, the main material starch was translated into soluble saccharides, which means the phenomenon called 'low temperature saccharification' appeared [24], and bulbs act as the source to provided energy for the whole plant's life activities [25,26]. At the end of vernalization, the content of soluble sugar, reducing sugar and sucrose reached a peak, indicating that the bulbs had broken dormancy and vernalization had finished [27]. Researchers have discovered that sugars, especially sucrose, may not only be energy substances, but also a signal to regulate plant growth and development [1].…”

Section: Effects Of Sut Genes On Bulb Development Of Lily Together Wimentioning

confidence: 99%

Transcriptome Analysis of Carbohydrate Metabolism Genes and Molecular Regulation of Sucrose Transport Gene LoSUT on the Flowering Process of Developing Oriental Hybrid Lily ‘Sorbonne’ Bulb

Zeng

Wang

et al. 2020

IJMS

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The quality of Lily cut flower was determined by the quality of bulbs. During the process of vernalization and flower bud differentiation, sugar massively accumulated in the bulb, which influenced the bulb development. However, the details of sugar genes’ regulation mechanism for these processes were not fully understood. Here, morphological physiology, transcriptomes and gene engineering technology were used to explore this physiological change. Seventy-two genes of 25 kinds of sugar metabolism-related genes were annotated after re-analyzing transcriptome data of Oriental hybrid lily ‘Sorbonne’ bulbs, which were generated on Hiseq Illumina 2000. The results showed that these genes were closely related to lily bulb vernalization and development. Combining gene expression pattern with gene co-expression network, five genes (Contig5669, Contig13319, Contig7715, Contig1420 and Contig87292) were considered to be the most potential signals, and the sucrose transporter gene (SUT) was the focus of this study. Carbohydrate transport pathway and genes’ regulation mechanism were inferred through a physiological and molecular test. SUT seemed to be the sugar sensor that could sense and regulate sugar concentration, which might have effects on other genes, such as FT, LFY and so on. LoSUT2 and LoSUT4 genes were cloned from Oriental hybrid lily ‘Sorbonne’ by RACE, which was the first time for these genes in Oriental hybrid lily ‘Sorbonne’. The physiological properties of these proteins were analyzed such as hydrophobicity and phosphorylation. In addition, secondary and tertiary structures of proteins were predicted, which indicated the two proteins were membrane proteins. Their cellular locations were verified through positioning the experiment of the fluorescent vector. They were highly expressed in cells around phloem, which illustrated the key role of these genes in sugar transport. Furthermore, transient expression assays showed that overexpressed LoSUT2 and LoSUT4 in Arabidopsis thaliana bloomed significantly earlier than the wild type and the expression of FT, SOC1 and LFY were also affected by LoSUT2 and LoSUT4, which indicated that LoSUT2 and LoSUT4 may regulate plants flowering time.

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“…A large amount of starch and soluble sugar is stored in the bulb scales. The scales supply the nutrients and energy for bulb dormancy and bud growth under low-temperature conditions [30] for completing generation. On the other hand, low temperature is also a stress signal which stimulates plant stress responses to avoid damages [31].…”

Section: Discussionmentioning

confidence: 99%

Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage

et al. 2022

IJMS

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Functional lilies are a group of edible lily cultivars with great potential for landscape application. Low-temperature storage can significantly improve their taste, but the knowledge of this process is largely unknown. In this study, we used the functional lilies ‘Fly Shaohua’ and ‘Fly Tiancheng’ as materials. Through physiological observation and transcriptome analysis during the bulbs’ cold storage, it was found that the starch degradation and sucrose accumulation in bulbs contributed to taste improvement. After 60 d of cold storage, the sucrose accumulation was highest and the starch content was lower in the bulbs, suggesting this time-point was optimal for consumption. Accompanying the fluctuation of sucrose content during cold storage, the enzyme activities of sucrose phosphate synthase and sucrose synthase for sucrose synthesis were increased. Transcriptome analysis showed that many differentially expressed genes (DEGs) were involved in the starch and sucrose metabolism pathway, which might promote the conversion of starch to sucrose in bulbs. In addition, the DEGs involved in dormancy and stress response were also determined during cold storage, which might explain the decreased sucrose accumulation with extended storage time over 60 d due to the energy consumption for dormancy release. Taken together, our results indicated sucrose accumulation was a main factor in the taste improvement of lily bulbs after cold storage, which is attributable to the different gene expression of starch and sucrose metabolism pathways in this process.

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Dynamic changes in α- and β-amylase activities and gene expression in bulbs of the Oriental hybrid lily ‘Siberia’ during dormancy release

Cited by 5 publications

References 28 publications

ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective

ABA and Bud Dormancy in Perennials: Current Knowledge and Future Perspective

Transcriptome Analysis of Carbohydrate Metabolism Genes and Molecular Regulation of Sucrose Transport Gene LoSUT on the Flowering Process of Developing Oriental Hybrid Lily ‘Sorbonne’ Bulb

Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage

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