Dynamic changes in stanniocalcin gene expression in the mouse uterus during early implantation

Stasko, Sasha E.; DiMattia, Gabriel E.; Wagner, Graham F.

doi:10.1016/s0303-7207(00)00394-4

Cited by 34 publications

(32 citation statements)

References 13 publications

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“…It is possible that STC-1 synthesized in the stromal cells at the implantation site may be transferred to or sequestered by luminal epithelium and embryos. A different localization of STC-1 mRNA and protein was noticed in the mouse uterus, where the STC-1 mRNA was uniquely expressed in the mesometrial stromal cells, while STC-1 protein was also localized in the decidualizing antimesometrial cells and the adjacent luminal epithelial cells (Stasko et al 2001). The same mRNA-protein disparity in the adult mouse ovary was also reported by Deol et al (2000).…”

Section: Stanniocalcin 1 and 2 Expression In Rat Uterussupporting

confidence: 57%

“…This gene is also highly expressed during embryogenesis, particularly in the urogenital system, suggesting that STC-1 probably acts as a signaling molecule during embryo development (Varghese et al 1998, Stasko & Wagner et al 2001a. Recently, it has been observed that STC-1 is expressed uniquely in mouse mesometrial stromal cells and sequestered by decidualizing anti-mesometrial cells, indicating a possible role in decidualization and uterine remodeling (Stasko et al 2001). The expression and regulation of STC-1 in rat uterus during early pregnancy is still undefined.…”

Section: Introductionmentioning

confidence: 99%

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Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization

Xiao¹,

Yuan²,

Song³

et al. 2006

Reproduction

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Stanniocalcin-1 (STC-1) is a recently discovered polypeptide hormone, while stanniocalcin-2 (STC-2) is a subsequently identified homologue of stanniocalcin-1. Although previous studies have shown that both STC-1 and -2 are involved in various physiological processes, such as ion transport, reproduction and development, their expression in the uterus and roles in implantation and early pregnancy are unclear. Here we have investigated the expression and regulation of both STC-1 and STC-2 in rat uterus during early pregnancy under various physiological conditions. We show that only basal levels of STC-1 and STC-2 mRNA were detected in the uterus from day one (D1) to day five (D5) of pregnancy. STC-2 immunostaining was gradually increased in the glandular epithelium from day two (D2), with a peak occurring on D5. High levels of both STC-1 and STC-2 mRNA were observed in the stoma cells at the implantation site on day six (D6) of pregnancy, whereas their immunostaining signals were also significant in the luminal epithelium. Basal levels of both STC-1 and STC-2 mRNA and STC-1 immunostaining were detected in the uterus with delayed implantation. After the delayed implantation was terminated by estrogen treatment, both STC-1 and STC-2 mRNA signals were significantly induced in the stroma underlying the luminal epithelium at the implantation site, and STC-2 immunostaining was also observed in the luminal epithelium surrounding the implanting blastocyst. Embryo transfer experiments further confirmed that STC-1 and STC-2 expression at the implantation sites was induced by the implanting blastocyst. Both STC-1 mRNA and immunostaining were seen in the decidualized cells from day seven (D7) to day nine (D9) of pregnancy. STC-2 mRNA was also found in the whole decidua from D7 to D9 of pregnancy; STC-2 protein, however, was strictly localized to the primary deciduas on D7 and D8, with a weak expression in the whole deciduas on D9. Consistent with the normal pregnancy process, strong STC-1 and STC-2 mRNA signals were detected in the decidualized cells under artificial decidualization, whereas only basal levels of STC-1 mRNA and immunostaining were observed in the control horn. These data suggest, for the first time, that STC-1 together with STC-2 may play important roles in the processes of implantation and decidualization in the rat.

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Section: Stanniocalcin 1 and 2 Expression In Rat Uterussupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization

Xiao¹,

Yuan²,

Song³

et al. 2006

Reproduction

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“…STC1 appears to be involved in uterine remodeling following implantation. In the non-pregnant mouse uterus, STC1 mRNA and protein were found mainly in luminal epithelium (Stasko et al 2001). On day 5 of pregnancy blastocyst implantation was accompanied by a shift in STC1 mRNA expression from the epithelium to adjacent stromal cells, but most of the STC1 protein was found in the epithelial cells (Stasko et al 2001).…”

Section: Other Tissuesmentioning

confidence: 99%

“…In the non-pregnant mouse uterus, STC1 mRNA and protein were found mainly in luminal epithelium (Stasko et al 2001). On day 5 of pregnancy blastocyst implantation was accompanied by a shift in STC1 mRNA expression from the epithelium to adjacent stromal cells, but most of the STC1 protein was found in the epithelial cells (Stasko et al 2001). This pattern of expression in one cell type and accumulation in another resembles the situation in ovary and kidney, and suggests that STC1 is involved in mesenchymal-epithelial signaling in the mouse uterus.…”

Section: Other Tissuesmentioning

confidence: 99%

Mammalian stanniocalcins and cancer.

Chang

Jellinek²,

Reddel

2003

Endocrine-Related Cancer

145

129

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Stanniocalcin (STC) is a glycoprotein hormone that is secreted by the corpuscle of Stannius, an endocrine gland of bony fish, and is involved in calcium and phosphate homeostasis. The related mammalian proteins, STC1 and STC2, are expressed in a wide variety of tissues. The ovaries have the highest level of STC1, and this increases during pregnancy and lactation. STC1 is present in breast ductal epithelium, and its expression is induced by BRCA1, a tumor suppressor gene that has an important role in breast and ovarian cancer. The expression of STC2 is induced by estrogen, and there is a positive correlation between the level of expression of estrogen receptor and expression of both STC1 and STC2 in breast cancer. This article reviews the data currently available regarding the mammalian STCs, and discusses the roles they may play in normal physiology and in breast and other cancers.

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“…However, the molecules and pathways involved in the calcium homeostasis regulation are far away from being well elaborated. In rodent uterus during early pregnancy, it has been reported that stanniocalcin-1 (STC-1), which is one of the calcium regulation polypeptides, is expressed uniquely in mouse mesometrial stromal cells and sequestered by decidualizing antimesometrial cells, suggesting that STC-1 may play a role in decidualization and uterine tissue remodelling (Stasko et al 2001). Our unpublished data (L-J Xiao, X-X Song, Y-C Li, J-X Yuan, Z-Y Hu & Y-X Liu) in rat further confirmed that STC-1 was regulated during the onset of implantation and may be involved in the processes of implantation and decidulization.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Ca2+-sensing receptor expression and hormonal regulation in rat uterus during the peri-implantation period

Xiao¹,

Yuan²,

Li³

et al. 2005

Reproduction

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The extracellular Ca 21 -sensing receptor (CaR) is a member of the superfamily of G protein-coupled receptors (GPCRs). It is an important mediator of a wide range of Ca 21 -dependent physiological responses in various tissues. In reproductive tissues it has been reported to play a significant role in promoting or maintaining placentation. Meanwhile, another Ca 21 regulated gene stanniocalcin-1 (STC-1) has been documented to be involved in decidualization and uterine remodelling. The phenomenon that CaR mediates STC-1's transcription responding to extracellular calcium in fish urges us to suppose that CaR, like STC-1, may also play a role in implantation and decidualization. To resolve this conjecture, we have examined the expression and hormonal regulation of the CaR gene in rat uterus during peri-implantation period. CaR mRNA was expressed at a moderate level in the luminal epithelium of the early stage of pregnancy (from day 1 to day 3). From day 2-3 it began to be expressed more strongly in the stromal cells immediately underneath the luminal epithelium, but decreased to a basal level on day 4. From day 6 to day 9 continuously, both CaR mRNA and protein were highly expressed in the primary decidua. Expression of CaR mRNA and protein in these cells was also observed when a delayed implantation was terminated by estrogen treatment to allow the embryo implantation. In contrast, only basal level expression of the molecules was detected in the cells of animals subjected to a normal-delayed implantation or the pseudopregnant condition. Embryo transplantation experiment confirmed that CaR expression at the implantation site was induced by the implanting blastocyst. Consistent with the normal pregnant process, CaR mRNA and protein in the cells were also induced by an artificial decidualization procedure. Further experiments demonstrated that treatment of the ovariectomized rat with estrogen or/and progesterone stimulated a high level expression of CaR mRNA in the uterine epithelial and glandular epithelium. In conclusion, CaR was specifically induced during the processes of implantation and subsequent decidualization and may play a role in these processes.Reproduction (2005) 129 779-788

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Dynamic changes in stanniocalcin gene expression in the mouse uterus during early implantation

Cited by 34 publications

References 13 publications

Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization

Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization

Mammalian stanniocalcins and cancer.

Extracellular Ca2+-sensing receptor expression and hormonal regulation in rat uterus during the peri-implantation period

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