Dynamic Changes in Mitochondrial Nucleoids during the Transition from Anaerobic to Aerobic Culture in the Yeast Saccharomyces cerevisiae

Shiiba, Daisuke; Miyakawa, Isamu; Sando, Nobundo

doi:10.1508/cytologia.70.287

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…4BCD). 81 ) The highly aggregated mt-nucleoids could be isolated from anaerobically grown cells with sucrose gradient centrifugations. 82 ) This indicated that the highly aggregated mt-nucleoids observed by DAPI staining are not the apparent assembly of small mt-nucleoids.…”

Section: Dynamic Changes In Size and Number Of Mt-nucleoidsmentioning

confidence: 99%

Organization and dynamics of yeast mitochondrial nucleoids

Miyakawa

2017

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Mitochondrial DNA (mtDNA) is packaged by association with specific proteins in compact DNA-protein complexes named mitochondrial nucleoids (mt-nucleoids). The budding yeast Saccharomyces cerevisiae is able to grow either aerobically or anaerobically. Due to this characteristic, S. cerevisiae has been extensively used as a model organism to study genetics, morphology and biochemistry of mitochondria for a long time. Mitochondria of S. cerevisiae frequently fuse and divide, and perform dynamic morphological changes depending on the culture conditions and the stage of life cycle of the yeast cells. The mt-nucleoids also dynamically change their morphology, accompanying morphological changes of mitochondria. The mt-nucleoids have been isolated morphologically intact and functional analyses of mt-nucleoid proteins have been extensively performed. These studies have revealed that the functions of mt-nucleoid proteins are essential for maintenance of mtDNA. The aims of this review are to summarize the history on the research of yeast mt-nucleoids as well as recent findings on the organization of the mt-nucleoids and mitochondrial dynamics.

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Section: Dynamic Changes In Size and Number Of Mt-nucleoidsmentioning

confidence: 99%

Organization and dynamics of yeast mitochondrial nucleoids

Miyakawa

2017

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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“…These two species of proteins were also reduced in highly aggregated mt-nucleoids in rho + cells grown anaerobically. The amounts of these two proteins increased in the mt-nucleoid fractions, when anaerobically grown cells were transferred to aerobic conditions, accompanying the morphological changes of mt-nucleoids from aggregated forms to small spherical ones (Shiiba, Miyakawa and Sando 2005). These results suggested that at least two species of the mt-nucleoid proteins might be involved in morphological changes in mt-nucleoids.…”

Section: Discussionmentioning

confidence: 79%

Morphology of mitochondrial nucleoids in respiratory-deficient yeast cells varies depending on the unit length of the mitochondrial DNA sequence

Okamoto¹,

Inai²,

Miyakawa³

2016

FEMS Yeast Research

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One sentence summary: The authors investigated the mitochondrial nucleoids in Saccharomyces cerevisiae and found that the morphology of mitochondrial nucleoids in rho -cells significantly varies depending on the unit length of their mtDNA.Editor: Monique Bolotin-Fukuhara ABSTRACTWe investigated the morphology of mitochondrial nucleoids (mt-nucleoids) and mitochondria in Saccharomyces cerevisiae rho + and rho − cells with DAPI staining and mitochondria-targeted GFP. Whereas the mt-nucleoids appeared as strings of beads in wild-type rho + cells at log phase, the mt-nucleoids in hypersuppressive rho − cells (HS40 rho − cells) appeared as distinct punctate structures. In order to elucidate whether the punctate mt-nucleoids are common to other rho − cells, we observed the mt-nucleoids in rho − strains that retain different unit lengths of the mitochondrial DNA (mtDNA) sequence.As a result, rho − cells that have long mtDNA sequences, of more than 30 kb, had mt-nucleoids with a strings-of-beads appearance in tubular mitochondria. In contrast, rho − cells that have short mtDNA sequences, of <1 kb, had punctate mt-nucleoids in tubular mitochondria. This indicates that the morphology of mt-nucleoids in rho − cells significantly varies depending on the unit length of their mtDNA sequence. Analyses of mt-nucleoids suggest that the punctate mt-nucleoids in HS40 rho − cells consist of concatemeric mtDNAs and oligomeric circular mtDNAs associated with Abf2p and other nucleoid proteins.

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“…Recently, it has been reported that yeast cells alter the protein composition of mt-nucleoids depending on culture medium (fermentative or non-fermentative) (Kucej et al, 2008), and culture conditions (aerobic or anaerobic) (Shiiba et al, 1997(Shiiba et al, , 2005. The size and DNA content of individual mt-nucleoids also vary depending on the culture condition of cells .…”

Section: Morphology and Stability Of The Mt-nucleoids In A δAbf2 Mutantmentioning

confidence: 99%

Morphology and protein composition of the mitochondrial nucleoids in yeast cells lacking Abf2p, a high mobility group protein

Miyakawa

Kanayama

Fujita

et al. 2010

J. Gen. Appl. Microbiol.

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Dynamic Changes in Mitochondrial Nucleoids during the Transition from Anaerobic to Aerobic Culture in the Yeast Saccharomyces cerevisiae

Cited by 8 publications

References 24 publications

Organization and dynamics of yeast mitochondrial nucleoids

Organization and dynamics of yeast mitochondrial nucleoids

Morphology of mitochondrial nucleoids in respiratory-deficient yeast cells varies depending on the unit length of the mitochondrial DNA sequence

Morphology and protein composition of the mitochondrial nucleoids in yeast cells lacking Abf2p, a high mobility group protein

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