Dynamic changes in gene expression during human early embryo development: from fundamental aspects to clinical applications

Assou, Saïd; Boumela, I.; Haouzi, D.; Anahory, T.; Déchaud, H.; Vos, John De; Hamamah, S.

doi:10.1093/humupd/dmq036

Cited by 97 publications

(80 citation statements)

References 155 publications

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“…However, w70-80% of in vitro-produced embryos fail to implant and 66% of IVF cycles fail to result in pregnancy (Patrizio & Sakkas 2009, Assou et al 2011. This low efficiency contributes to the practice of multiple embryo transfer, which seeks to overcome implantation failure and maximize pregnancy rates.…”

Section: Introductionmentioning

confidence: 99%

“…Morphological assessment is the easiest way to predict viability, but most studies suggest that the morphology of embryos with high-quality morphological appearance is insufficient to predict a successful implantation (Katz-Jaffe et al 2009, Assou et al 2011, Mastenbroek et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, morphologically normal embryos can be genetically abnormal, as a significant proportion of aneuploid embryos is capable of achieving the highest morphological scores (Singh & Sinclair 2007, Alfarawati et al 2011, Assou et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…The limitations of strategies based on morphological embryo grading at day 3 embryos have prompted the investigation of adjunctive technologies for the noninvasive biochemical assessment of embryo viability in ART (Hamel et al 2008, Katz-Jaffe et al 2009, Aydiner et al 2010, Assou et al 2011. These approaches include the measurement of glucose, lactate, pyruvate, or amino acid levels in the embryo culture media, as well as the assessment of oxygen consumption by the embryo, and genomic and proteomic profiling (Gardner et al 2001, Houghton et al 2002, Brison et al 2004, Lopes et al 2005, Dominguez et al 2008, Katz-Jaffe et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…As the number of ART cycles increases worldwide, improvement of the ability to perform fast, reliable, and noninvasive embryo viability prediction has become a crucial target for reproductive medicine (Aydiner et al 2010, Assou et al 2011. Mass spectrometry (MS) fingerprinting is a global screening approach that compares and classifies samples based on their metabolite patterns.…”

Section: Introductionmentioning

confidence: 99%

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Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium

Cortezzi¹,

Cabral²,

Trevisan³

et al. 2013

Reproduction

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This study has evaluated the performance of a multivariate statistical model to predict embryo implantation potential by processing data from the chemical fingerprinting of culture medium samples used for human embryo culture. The culture medium for 113 embryos from 55 patients undergoing ICSI was collected after embryo transfer. The samples were split into positive (nZ29) and negative (nZ84) implantation groups according their implantation outcomes (100% or 0% implantation). The samples were individually diluted and analyzed by electrospray ionization mass spectrometry (ESI-MS). The m/z ratios and relative abundances of the major ions in each spectrum were considered for partial least square discriminant analysis. Data were divided into two subsets (calibration and validation), and the models were evaluated and applied to the validation set. A total of 5987 ions were observed in the groups. The multivariate statistical model described more than 82% of the data variability. Samples of the positive group were correctly identified with 100% probability and negative samples with 70%. The culture media used for embryos that were positive or negative for successful implantation showed specific biochemical signatures that could be detected in a fast, simple, and noninvasive way by ESI-MS. To our knowledge, this is the first report that uses MS fingerprinting to predict human embryo implantation potential. This biochemical profile could help the selection of the most viable embryo, improving single-embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium

Cortezzi¹,

Cabral²,

Trevisan³

et al. 2013

Reproduction

View full text Add to dashboard Cite

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Clinical Application of Methods to Select In Vitro Fertilized Embryos

2016

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Maternal genetic effect in DNA analysis: Egg on your traits

Wilson

2012

American J of Med Genetics Pt A

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A dilemma of penetrance parallels the uncertainty principle as array analyses uncover a disturbing continuity of human molecular variation. Among mechanisms for altered penetrance is maternal genetic effect, hypothesized to explain mother-offspring discordance from 12/13 translocation [Wilson et al., 1991] and important to consider when confronting identical genetic changes in normal mothers and their abnormal children. A boy with developmental delays, autistic features, minimal dysmorphology, and maternally inherited 17p13.3 microduplication could exemplify maternal genetic effect, as could a boy with altered brain pattern and maternally inherited Sonic Hedgehog mutation reported by Schell-Apacik et al. [2009]. Maternal genetic effects, characterized in fly, fish, and mouse, are important to consider in human DNA analysis so that identical findings in mother and child are not routinely dismissed as benign variations. Suspect families can define relevant maternal-zygote transition (MZT) genes, provide assays for oocyte function and disruption, and shift anomaly emphasis from pattern recognition to pattern restoration.

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Dynamic changes in gene expression during human early embryo development: from fundamental aspects to clinical applications

Abstract: Omics analysis provides tools for understanding the molecular mechanisms and signaling pathways controlling early embryonic development. Furthermore, we discuss the clinical relevance of using a non-invasive molecular approach to embryo selection for the single-embryo transfer program.

Cited by 97 publications

References 155 publications

Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium

Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium

Clinical Application of Methods to Select In Vitro Fertilized Embryos

Maternal genetic effect in DNA analysis: Egg on your traits

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