Dynamic changes in flow rate and composition of urine during the post-bloodmeal diuresis inAedes aegypti (L.)

Williams, James C.; Hagedorn, Henry H.; Beyenbach, Klaus W.

doi:10.1007/bf00689629

Cited by 111 publications

(109 citation statements)

References 13 publications

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“…aegypti (6-7 fi\;Briegel 1985). Regular diuresis, initiated only after termination of feeding in Aedes (Boorman 1960, Stobbart 1977, Jones & Brandt 1981, Williams et al 1983 as well as in Anopheles (Nijhout & Carrow 1978), serves primarily to reduce the flight weight of the newly fed female. The prediuretic excretion reported here, however, seems to be an additional adaptation evolved by these mosquitoes primarily to compensate for the smaller volume of the midgut and/or its limited elasticity.…”

Section: Discussionmentioning

confidence: 99%

“…As in many hematophagous insects, e.g., Rhodnius (Maddrell 1964) and Glossina (Gee 1975), a conspicuous diuresis begins in mosquitoes shortly after ingestion of blood (Boorman 1960, Stobbart 1977, Jones & Brandt 1981, Williams et al 1983). Nijhout & Carrow (1978) have investigated diuresis in Anopheles freeborni Aitken and ascribed its control to a diuretic hormone.…”

mentioning

confidence: 99%

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Concentration of Host Blood Protein During Feeding by Anopheline Mosquitoes (Diptera: Culicidae)1

Briegel¹,

Rezzonico

1985

Journal of Medical Entomology

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Concentration of Host Blood Protein During Feeding by Anopheline Mosquitoes (Diptera: Culicidae)1

Briegel¹,

Rezzonico

1985

Journal of Medical Entomology

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“…The mosquito counteracts this problem by excreting 40% of the meal's water content within two hours (Williams et al, 1983). The Na + /K + -ATPase located on the basolateral membrane of the posterior midgut may contribute to diuresis (Sanders et al, 2003;Patrick et al, 2006).…”

Section: Diuresismentioning

confidence: 99%

“…Through the activation of G-protein coupled receptors and the release of intracellular Ca 2+ stores, the paracellular resistance of the Malpighian tubules decreases tenfold leading to a "leaky epithelium" that allows a dramatic increase in the transepithelial secretion of NaCl, KCl and water which are then eliminated by the mosquito (Beyebach, 2012). The peak phase of diuresis occurs around six minutes after the acquisition of a blood meal and steadily declines until two hours after feeding (Williams et al, 1983). It is interesting to note that the mosquito does not completely excrete the entire plasma/water portion of the meal, but begins to reduce the number of water-transporting aquaporins in the posterior midgut after diuresis (Sanders et al, 2003;Drake et al, 2010).…”

Section: Diuresismentioning

confidence: 99%

Transport of H+, Na+ and K+ across the posterior midgut of blood-fed mosquitoes (Aedes aegypti)

Pacey

O’Donnell

2014

Journal of Insect Physiology

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Mosquitoes pose significant threats to human health because they act as vectors for disease causing viruses and protozoans. Indeed, Aedes aegypti is known as the Yellow Fever Mosquito because of its role as a vector for viral infections that kill thousands of people each year. A more thorough understanding of mosquito physiology will aid development of novel control strategies. Previous work on ion transport across the midgut has been focused primarily on larval A. aegypti, while research on the midgut of the adult stage is less complete. The posterior midgut of the adult female is of particular interest because it is used for the storage and digestion of the blood meal which is required for the production of eggs. This study used an array of electrophysiological methodologies such as the Scanning Ion Electrode Technique (SIET) in order to elucidate the patterns and mechanisms of Na + , H + and K + transport across the posterior midgut at intervals during postprandial diuresis and digestion of the blood meal. Measurements of transepithelial potential indicated that the lumen was at its most negative (-13.2 mV) three hours after the blood meal and then gradually became less negative during the time course of digestion. Na + was absorbed (from lumen to bath) at all intervals after the blood meal (6 min, 30 min, 2h, 24 h); calculations of the electrochemical potential indicated that absorption required active transport. H + absorption at all times (6 min -48 h) after the blood meal was also active (i.e. against the electrochemical gradient for H + ) and was greatly reduced by inhibition of carbonic anhydrase. K + transport across the midgut exhibited two distinct phases. During diuresis, luminal concentrations of K + were in the range 24 -28 mM and secretion into the midgut was opposed by the electrochemical iv gradient, indicating active transport. After diuresis, during blood meal digestion, concentrations of K + in the midgut contents were high (95 -134 mM) and absorption of K + was favoured by the electrochemical gradient. K + absorption was sensitive to the channel blocker Ba 2+ during this period.v Acknowledgements

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“…Imbibing a huge blood meal also quickly generates osmotic stress that requires an efficient excretory system for rapid removal of excess water (2)(3)(4)(5). However, one overlooked challenge of feeding on mammalian blood is the high temperature of the blood that rushes into the gut.…”

mentioning

confidence: 99%

Drinking a hot blood meal elicits a protective heat shock response in mosquitoes

Benoit

López‐Martínez

Patrick

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

131

106

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The mosquito's body temperature increases dramatically when it takes a blood meal from a warm-blooded, vertebrate host. By using the yellow fever mosquito, Aedes aegypti, we demonstrate that this boost in temperature following a blood meal prompts the synthesis of heat shock protein 70 (Hsp70). This response, elicited by the temperature of the blood meal, is most robust in the mosquito's midgut. When RNA interference is used to suppress expression of hsp70, protein digestion of the blood meal is impaired, leading to production of fewer eggs. We propose that Hsp70 protects the mosquito midgut from the temperature stress incurred by drinking a hot blood meal. Similar increases in hsp70 were documented immediately after blood feeding in two other mosquitoes (Culex pipiens and Anopheles gambiae) and the bed bug, Cimex lectularius, suggesting that this is a common protective response in blood-feeding arthropods.

show abstract

Dynamic changes in flow rate and composition of urine during the post-bloodmeal diuresis inAedes aegypti (L.)

Cited by 111 publications

References 13 publications

Concentration of Host Blood Protein During Feeding by Anopheline Mosquitoes (Diptera: Culicidae)1

Concentration of Host Blood Protein During Feeding by Anopheline Mosquitoes (Diptera: Culicidae)1

Transport of H+, Na+ and K+ across the posterior midgut of blood-fed mosquitoes (Aedes aegypti)

Drinking a hot blood meal elicits a protective heat shock response in mosquitoes

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