Dynamic change of the rhizosphere microbial community in response to growth stages of consecutively monocultured Rehmanniae glutinosa

Abstract

Abstract, an important medical plant in China, has distinct regional characteristics. It has long suffered from consecutive monoculture obstacles, resulting in severe reduction of quality and yield. The microbial community is believed to play an important role in the monoculture process. However, there are no reports on how microbial compositions change in response to growth of

Barcoded Pyrosequencing Reveals a Shift in the Bacterial Community in the Rhizosphere and Rhizoplane of Rehmannia glutinosa under Consecutive Monoculture

“…Xiong et al [ 28 ] compared microbial communities in suppressive- and conducive-soils associated with Fusarium wilt disease and found that Actinobacteria and Firmicutes were strongly enriched in the suppressive soil in a vanilla long-term continuous cropping system. Yang et al [ 18 ] indicated that some probiotic bacteria belonging to Actinobacteria significantly decreased with the extended monoculture of R. glutinosa . In addition, disease suppression that is induced by bioorganic fertilizer application in Nicotiana tabacum and Persea americana was associated with an increase in multiple beneficial strains such as Actinobacteria , Firmicutes , etc.…”

“…However, the rhizoplane of plant root systems plays a selective gating role in microbiome recruitment from soil [ 19 ] depending on the root phenotypic traits [ 20 ]. This aspect was not studied in previous studies [ 18 ]. In addition, antagonistic activities of beneficial microbial biomarkers in disease-suppressive soils are still poorly understood [ 18 ].…”

“…This aspect was not studied in previous studies [ 18 ]. In addition, antagonistic activities of beneficial microbial biomarkers in disease-suppressive soils are still poorly understood [ 18 ]. Even though severe replant disease is observed in the field and confirmed by the detection of specific fungal pathogens ( F. oxysporum and A. flavus ) by quantitative PCR [ 3 ], little is known about the shifts in root-associated bacterial communities and their relationships with the increase of some specific soil-borne pathogens (i.e., F. oxysporum and A. flavus ) under consecutive monoculture.…”

“…High-throughput pyrosequencing of 16S rRNA gene fragments has proven to be a powerful tool for in-depth sequencing and parallel analysis of soil and root microbial communities at a reduced sequencing error rate [ 16 , 17 ]. Illumina pyrosequencing was applied for the study of soil microbial communities under R. glutinosa monoculture [ 18 ]. However, the rhizoplane of plant root systems plays a selective gating role in microbiome recruitment from soil [ 19 ] depending on the root phenotypic traits [ 20 ].…”

Comparative Metagenomic Analysis of Rhizosphere Microbial Community Composition and Functional Potentials under Rehmannia glutinosa Consecutive Monoculture

“…Using Illumina sequencing of small subunit ribosomal RNA (SSU rRNA) genes, previous studies demonstrated that consecutive monoculture of R. glutinosa altered the microbial community structure in rhizosphere soil, and led to the imbalance of the microbial community [ 8 , 20 ]. However, there exists limited knowledge of the functional attributes of root-associated microbes in the rhizosphere soil of R. glutinosa , and the linkages between the variation of functional genes and the dynamics of microbial community.…”