Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage

Chavez, Shawn L.; Loewke, Kevin; Han, Jinnuo; Moussavi, Farshid; Colls, P.; Munné, Santiago; Behr, Barry; Pera, Renee A. Reijo

doi:10.1038/ncomms2249

Cited by 281 publications

(329 citation statements)

References 52 publications

(90 reference statements)

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“…Mertzanidou et al found that 3/27 (11%) of cleavage stage embryos carried a meiotic abnormality, a figure that is in line with other researchers, for instance Chavez et al [45] who found 9/45 four-cell embryos to carry a meiotic abnormality. The majority of abnormalities found were therefore of post-zygotic and mosaic in origin: between 46% [45] and 87% [10] seem to be mitotic abnormalities. Mitotic non-disjunction seemed to be less frequent than previously assumed, and endoreduplication followed by a cellular division with multipolar spindles was proposed as a mechanism leading to chaotic karyotypes [9].…”

Section: Array Comparative Genomic Hybridisation (Acgh)supporting

confidence: 75%

“…Array CGH was also an important tool to start to understand the origin of post-zygotic chromosomal abnormalities in embryos. By arraying every cell of cleavage stage embryos, it was found that the majority of cleavage stage embryos were chromosomally abnormal [9,10,[44][45][46]. Mertzanidou et al found that 3/27 (11%) of cleavage stage embryos carried a meiotic abnormality, a figure that is in line with other researchers, for instance Chavez et al [45] who found 9/45 four-cell embryos to carry a meiotic abnormality.…”

Section: Array Comparative Genomic Hybridisation (Acgh)supporting

confidence: 60%

“…By arraying every cell of cleavage stage embryos, it was found that the majority of cleavage stage embryos were chromosomally abnormal [9,10,[44][45][46]. Mertzanidou et al found that 3/27 (11%) of cleavage stage embryos carried a meiotic abnormality, a figure that is in line with other researchers, for instance Chavez et al [45] who found 9/45 four-cell embryos to carry a meiotic abnormality. The majority of abnormalities found were therefore of post-zygotic and mosaic in origin: between 46% [45] and 87% [10] seem to be mitotic abnormalities.…”

Section: Array Comparative Genomic Hybridisation (Acgh)supporting

confidence: 60%

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Preimplantation Genetic Screening

Sermon¹

2017

obm genet

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The main aim of PGS has always been to improve IVF outcome, especially in patient groups assumed to have higher rates of chromosomally abnormal embryos, such as patients of advanced maternal age. In that sense, PGS is quite different from other types of screening as discussed in other papers in this issue. Today it bears no doubt that blastocysts found to be uniformly aneuploid in a biopsy will fail to implant, or worse, will implant and lead to a pregnancy and birth carrying a major chromosomal abnormality, such as trisomy 21. However, it has been argued that a cohort of embryos cannot be improved, and that PGS is only a selection method for which efficiency has not been proven. PGS would never increase the live birth rate for that given cohort, even with a 100% efficiency rate of embryo cryopreservation. The current debate on whether PGS should be applied and to which patients it should be offered has shifted from the effect on live birth rates towards other outcomes such as the reduction of transfers and of miscarriages. Taking the undeniable higher cost of IVF into account when PGS is included, what is the benefit to the patient? The views on this question differ on whether PGS is an additional source of income for the IVF clinic and may or may not balance the extra cost for cryopreservation and embryo transfer for the patients, or whether society pays for IVF treatments and may decide not to want to invest in a medical act that does not improve the primary goal of IVF, i.e. having a healthy child. PGS is also often presented as diminishing patient anxiety and stress through decreasing unnecessary embryos transfers and miscarriages, although no data on this

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Section: Array Comparative Genomic Hybridisation (Acgh)supporting

confidence: 75%

Section: Array Comparative Genomic Hybridisation (Acgh)supporting

confidence: 60%

Section: Array Comparative Genomic Hybridisation (Acgh)supporting

confidence: 60%

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Preimplantation Genetic Screening

Sermon¹

2017

obm genet

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“…This suggests abnormal distribution of chromosomes during first mitosis. Chavez et al [10] observed that the generation of partial chromosomal gains and losses were restricted primarily to embryos with mitotic errors. During the first mitotic divisions in human preimplantation development, improper segregation of chromosomes might occur, causing high rates of aneuploidy and mosaicism [11].…”

Section: Resultsmentioning

confidence: 99%

Irregular cleavage of early preimplantation human embryos: characteristics of patients and pregnancy outcomes

Almagor

Fieldust

et al. 2015

J Assist Reprod Genet

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Purpose This is a retrospective analysis of the morphokinetics, prevalence, and implantation potential of embryos with irregular first and second cleavages as identified by time-lapse microscopy. Methods The study included 253 women who underwent 387 assisted reproduction treatments with intracytoplasmic sperm injection (ICSI). Each patient was assigned to one of three groups based on embryo cleavage results. In group I, one to two embryos per cycle showed irregular cleavage; group II, at least three embryos with abnormal cleavage; and in group III (the control group), all embryos cleaved normally. The number of embryos that cleaved from 1 to ≥3 cells or from 2 to ≥5 cells for each patient was recorded. Their prevalence and association with women's characteristics and pregnancy outcome were evaluated. Results The prevalence of irregular cleavage was 15.6 % among 1772 ICSI embryos. In 101 cycles, 1-2 embryos per cycle showed irregular cleavage (group I). In 32 cycles, at least 3 embryos showed abnormal cleavage (group II). In 254 cycles, all embryos cleaved normally (group III). The average age of the women in group II was significantly lower in comparison with groups I and III (32.5±4.2 vs. 35.1±4.9 and 35.5±5.1, respectively, p<0.02). In comparison of groups I and II, the odds ratio for ≥3 embryos with irregular cleavage in women younger than 35 was 3.48 (95 % CI, 1.28 to 9.46). Embryos with irregular cleavage were transferred in 16 women. Three live births were achieved following the transfer of single blastocysts derived from embryos with irregular cleavage from two to five cells. Conclusions Early embryos with irregular cleavage are significantly more prevalent in younger women. When these embryos develop to the blastocyst stage, they may have normal implantation potential, leading to the birth of healthy babies.

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“…The timing of key parameters from time-lapse monitoring for predictions of blastocyst formation [11][12][13] aneuploidy, [14,15], and, in some cases, implantation [16][17][18] have been investigated. Various models based on specific developmental milestones and phenotypes have been built [9].…”

Section: Available Time-lapse Systems and Potential Benefitsmentioning

confidence: 99%

A critical appraisal of time-lapse imaging for embryo selection: where are we and where do we need to go?

Racowsky

Kovács

Martins

2015

J Assist Reprod Genet

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Purpose The aim of this study was to undertake a critical appraisal of the available evidence for the use of time-lapse imaging for embryo selection in clinical IVF. Methods A literature search in PubMed, Scopus, Cochrane Central, ClinicalTrials.gov, Current Controlled Trials, and WHO International Clinical Trials Registry Platform was performed to identify randomized controlled trials that investigated the effect of time-lapse embryo selection and/or the timelapse incubation system on ongoing pregnancy rate. We then performed a systematic review and assessed the relative risks (RRs) and 95 % confidence intervals (CIs) for ongoing pregnancy rates and the risk of bias of the eligible studies. Results We identified four eligible randomized studies, three of which investigated the effect of both time-lapse incubation system and selection on ongoing pregnancy rate; the pooled result revealed a benefit of this intervention (relative risk (RR) 1.20; 95 % CI 1.05-1.37). However, the evidence was judged to be of low quality due to study limitations; a beneficial effect was observed in only one study deemed to be at high risk of bias. The single study assessing the effect of only the timelapse incubation system revealed a non-significant negative effect (RR 0.71; 95 % CI 0.49-1.03). Conclusions The findings from this systematic review of the current evidence do not support routine use of time-lapse technology in clinical IVF. We therefore believe that the use of time-lapse imaging for embryo selection should remain experimental and that couples should not be subject to a surcharge for having their embryos cultured in a time-lapse imaging system. Future studies evaluating this technology in welldesigned trials should be performed.

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Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage

Cited by 281 publications

References 52 publications

Preimplantation Genetic Screening

Preimplantation Genetic Screening

Irregular cleavage of early preimplantation human embryos: characteristics of patients and pregnancy outcomes

A critical appraisal of time-lapse imaging for embryo selection: where are we and where do we need to go?

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