Dynamic bioengineered hydrogels as scaffolds for advanced stem cell and organoid culture

Bahlmann, Laura C.; Fokina, Ana; Shoichet, Molly S.

doi:10.1557/mrc.2017.72

Cited by 24 publications

(12 citation statements)

References 82 publications

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“…Generally, cells sense the bulk scaffold elasticity, while in viscoelastic materials they can further mechanically remodel their surrounding depending on the relaxation rate and cell type. [ 31 ] In future investigations, the mechanical properties of the CNF hydrogel could be adapted to suit the application of organoid expansion, and softened over time through controlled enzymatic degradation. Additionally, ECM components proven to be advantageous for organoid growth, such as laminin‐111, collagen IV, and fibronectin, could be incorporated.…”

Section: Discussionmentioning

confidence: 99%

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

Krüger

Oosterhoff

Wolferen

et al. 2020

Adv Healthcare Materials

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To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting. Recently, adult liver‐derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte‐like fate. The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable. Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids. The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte‐like cells compared to MG. Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.

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Section: Discussionmentioning

confidence: 99%

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

Krüger

Oosterhoff

Wolferen

et al. 2020

Adv Healthcare Materials

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“…Most complex organs, such as the lungs, kidneys, and glands, are formed by several branched, independent, organized cellular aggregates. In order to reproduce organ-specific microstructures, biomaterials should be versatile enough to allow the growth and self-organization of several cellular subtypes [46,[161][162][163]. This implies the following matrix rearrangement according to the stochastic cell growth.…”

Section: Advances In Polymer Technologymentioning

confidence: 99%

Supramolecular Biopolymers for Tissue Engineering

Pérez-Pedroza

Ávila-Ramírez

Khan

et al. 2021

Advances in Polymer Technology

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Supramolecular biopolymers (SBPs) are those polymeric units derived from macromolecules that can assemble with each other by noncovalent interactions. Macromolecular structures are commonly found in living systems such as proteins, DNA/RNA, and polysaccharides. Bioorganic chemistry allows the generation of sequence-specific supramolecular units like SBPs that can be tailored for novel applications in tissue engineering (TE). SBPs hold advantages over other conventional polymers previously used for TE; these materials can be easily functionalized; they are self-healing, biodegradable, stimuli-responsive, and nonimmunogenic. These characteristics are vital for the further development of current trends in TE, such as the use of pluripotent cells for organoid generation, cell-free scaffolds for tissue regeneration, patient-derived organ models, and controlled delivery systems of small molecules. In this review, we will analyse the 3 subtypes of SBPs: peptide-, nucleic acid-, and oligosaccharide-derived. Then, we will discuss the role that SBPs will be playing in TE as dynamic scaffolds, therapeutic scaffolds, and bioinks. Finally, we will describe possible outlooks of SBPs for TE.

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“…In the field of organoid culture, organogenesis and hydrogel design inform one another; spatiotemporally controlled mechanical and biochemical cues within hydrogels can reveal developmental phenomenon that would be difficult to study in conventional systems 74. Often, in vitro cancer models ignore the effects of matrix properties on cell behavior and the heterogeneity of tumors.…”

Section: Challenges and Opportunities For Cancer Invasion Modelsmentioning

confidence: 99%

Designer Biomaterials to Model Cancer Cell Invasion In Vitro: Predictive Tools or Just Pretty Pictures?

Bahlmann

Smith

Shoichet

2020

Adv Funct Materials

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Metastasis is the leading cause of mortality in cancer patients. Underlying this process is the invasion and colonization of cancer cells into healthy tissues. Engineered hydrogel models of tumor microenvironments present an opportunity to understand the microenvironmental determinants of cellular invasion. The biochemical and mechanical cues, presented in the form of adhesion sites, degradable cues, matrix stiffness, and architecture, have significant effects on the extent of cancer cell migration, and the mechanisms employed by these cells to move through their matrix. Coculture with stromal cells such as cancer associated fibroblasts, endothelial cells, and immune cells that are associated with poor prognosis demonstrate that these cells exacerbate cancer cell invasion. With these models, researchers aim not only to recapitulate known cancer cell behaviors in a dish, but also to uncover new insights into mechanisms underlying these phenomena, paving the way for novel treatment strategies. In this perspective, the design of engineered models that are used to study cancer cell invasion and metastasis in vitro is discussed. To this end, the authors seek to understand and put into perspective: do these models reveal relevant mechanisms of cancer cell migration, or are they simply pretty pictures with little biological translatability?

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Dynamic bioengineered hydrogels as scaffolds for advanced stem cell and organoid culture

Cited by 24 publications

References 82 publications

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

Supramolecular Biopolymers for Tissue Engineering

Designer Biomaterials to Model Cancer Cell Invasion In Vitro: Predictive Tools or Just Pretty Pictures?

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