Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing

Tvrdá, Eva; Arroyo, F.; Ďuračka, Michal; López‐Fernández, Carmen; Gosálvez, Jaime

doi:10.1007/s10815-019-01423-y

Cited by 8 publications

(6 citation statements)

References 50 publications

(79 reference statements)

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“…With regard to the effect of sperm concentration on DNA fragmentation, we observed that sDFI was higher when sperm concentration increased. It was observed that DNA longevity depends largely on sperm concentration during incubation [ 56 ]. After incubation at 37 °C, the DNA fragmentation rate was greater in samples with high sperm concentration, but it had a significant individual effect [ 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…It was observed that DNA longevity depends largely on sperm concentration during incubation [ 56 ]. After incubation at 37 °C, the DNA fragmentation rate was greater in samples with high sperm concentration, but it had a significant individual effect [ 56 , 57 ]. One explanation could be that an increase in oxidative stress (e.g., H 2 O 2 ) occurs when spermatozoa are stored at high concentrations [ 4 , 15 ], and it is known that an increase in oxidative stress induces nuclear and mitochondrial DNA damage [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

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Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Sadeghi

Gallego

García-Colomer

et al. 2020

Biology

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The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Sadeghi

Gallego

García-Colomer

et al. 2020

Biology

Self Cite

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“…All slides were stained using SYBR Green (2 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA) in VECTASHIELD (Vector Laboratories, Burlingame, CA, USA) and a minimum of 300 spermatozoa per sample was scored using an Epifluorescence Leica DMI6000 microscope using a dry ×40 fluorite magnification objective (Leica Microsystems, Wetzlar, Germany). The incidence of DNA fragmented spermatozoa was expressed in percentages (%) [42].…”

Section: Assessment Of Sperm Dna Integritymentioning

confidence: 99%

Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality

Tirpák

Halo

Tokárová

et al. 2021

Life

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The composition of seminal plasma of individual sires varies and so does the fertilizing ability. Micro and macro elements along with seminal enzymes, hormones, proteins, and lipids contained in seminal plasma are essential for the proper physiological function of spermatozoa. However, elevated levels against the normal physiological values, especially in the case of trace metals, result in the production of reactive oxygen species. The deficiency of antioxidants in the seminal plasma that could scavenge free radicals causes an impairment of spermatozoa quality. Ejaculates were obtained from 19 stallions. The fresh semen was analyzed to evaluate qualitative parameters of spermatozoa in terms of the motility, viability, and integrity of DNA. Separated seminal plasma underwent the assessment of the chemical and biochemical composition and RedOx markers. Based on the obtained concentrations of individual chemical elements, the correlation analysis suggested a negative impact of Cu in seminal plasma on the SOD, GPx, and LPO. Contrary, positive correlation was detected between FRAP and motility features. While Cu negatively correlated with sperm motion parameters, the adverse effect on viability was suggested for Cd. Our data suggest that seminal plasma has a potential due to its availability to become the potential biomarker of the reproductive health of farm animals.

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“…Analysis of the halo diameter was used as a quantitative parameter for DNA breaks (chromatin damage intensity), whereas the percentage of sperm with fragmented DNA was determined by classifying 400 sperm haloes using the criteria defined for sperm DNA fragmentation (40).…”

Section: Alkaline and Neutral Chromatin Dispersion Assaymentioning

confidence: 99%

Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage

et al. 2021

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Although sperm chromatin damage, understood as damage to DNA or affectations in sperm protamination, has been proposed as a biomarker for sperm quality in both humans and livestock, the low incidence found in some animals raises concerns about its potential value. In this context, as separate methods measure different facets of chromatin damage, their comparison is of vital importance. This work aims at analyzing eight techniques assessing chromatin damage in pig sperm. With this purpose, cryopreserved sperm samples from 16 boars were evaluated through the following assays: TUNEL, TUNEL with decondensation, SCSA, alkaline and neutral sperm chromatin dispersion (SCD) tests, alkaline and neutral Comet assays, and chromomycin A3 test (CMA3). In all cases, the extent of chromatin damage and the percentage of sperm with fragmented DNA were determined. The degree of chromatin damage and the percentage of sperm with fragmented DNA were significantly correlated (p < 0.05) in direct methods (TUNEL, TUNEL with decondensation, and alkaline and neutral Comet) and CMA3, but not in the indirect ones (SCD and SCSA). Percentages of sperm with fragmented DNA determined by alkaline Comet were significantly (p < 0.05) correlated with TUNEL following decondensation and CMA3; those determined by neutral Comet were correlated with the percentage of High DNA Stainability (SCSA); those determined by SCSA were correlated with neutral and alkaline SCD; and those determined by neutral SCD were correlated with alkaline SCD. While, in pigs, percentages of sperm with fragmented DNA are directly related to the extent of chromatin damage when direct methods are used, this is not the case for indirect techniques. Thus, the results obtained herein differ from those reported for humans in which TUNEL, SCSA, alkaline SCD, and alkaline Comet were found to be correlated. These findings may shed some light on the interpretation of these tests and provide some clues for the standardization of chromatin damage methods.

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Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing

Cited by 8 publications

References 50 publications

Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality

Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage

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