Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA)

Moniri, Mani Roshan; Young, Amelia G.; Reinheimer, Kelsey; Rayat, Jarrett; Dai, Long‐Jun; Warnock, Garth L.

doi:10.1007/s10616-014-9692-5

Cited by 112 publications

(69 citation statements)

References 16 publications

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“…We used RTCA, which is an impedance-based technology that can be used for label-free and real-time monitoring of cell properties, such as cell adherence, proliferation, migration and cytotoxicity. RTCA is also a powerful and reliable tool that can be used in drug discovery for toxicity and pharmacology studies434445. We found that SAHA treatment induced a time- and dose-dependent decrease in CI values in both cell lines.…”

Section: Discussionmentioning

confidence: 74%

Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells

Zhou

Feng

Han

et al. 2016

Sci Rep

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Previous studies showed that either histone deacetylase (HDAC) inhibitors or tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can induce apoptosis in tumor cells including breast cancer. However, the underling mechanisms of combining HDAC inhibitors with TRAIL in the treatment of breast cancer are poorly understood. In this study, we determined the ability of SAHA and TRAIL as single agents or in combination to inhibit the growth and survival of MCF-7 and MDA-MB-231 breast cancer cells. Our results demonstrate that the distinct effects of SAHA or TRAIL individually and in combination on the proliferation, cell viability, apoptosis, cell cycle distribution, and morphological changes of MDA-MB-231 and MCF-7 cells. We further determined the different effects of SAHA or TRAIL alone and combining SAHA with TRAIL on the expression of a number of apoptosis-related molecules, cell cycle, growth factors and their receptors in cancer cells. Our results demonstrated that the combinatorial treatment of SAHA and TRAIL may target multiple pathways and serve as an effective therapeutic strategy against breast cancer. An improved understanding of the molecular mechanisms may facilitate either SAHA or TRAIL targeted use and the selection of suitable combinations.

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Section: Discussionmentioning

confidence: 74%

Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells

Zhou

Feng

Han

et al. 2016

Sci Rep

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“…However, these end-point assays need to destroy cells, leading to cell death or the destruction of cell structure. The real time cell analyzer (RTCA, xCELLigence, Roche) is an impedance-based technology that can be used for label-free and real-time monitoring of cell properties, such as cell adherence, proliferation, migration, and cytotoxicity, which could have widespread use [31]. In the detection of apoptosis at the molecular level, the normally detected proteins are caspase-9, caspase-3, and PARP (substrates of caspase-3), located in the mitochondrial pathway [32].…”

Section: Discussionmentioning

confidence: 99%

The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells

Liang

Zhu

et al. 2019

Toxins

119

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Resveratrol, a natural polyterpenoid, can scavenge reactive oxygen species in vivo to carry out the functions of antioxidation and antiaging. Resveratrol's anti-cancer capability has attracted widespread attention, but its molecular mechanism has not been systematically explained. In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a timeand concentration-dependent manner. A total of 330 significantly differential genes were identified through large-scale transcriptome sequencing, among which 103 genes were upregulated and 227 genes were downregulated. Transcriptome and qRT-PCR data proved that a large number of genes related to cell cycle were differentially expressed after the treatment of resveratrol. The changes of cell cycle phases at different time points after treating with resveratrol were further detected, and it was found that the cells were arrested in the S phase because of the percentage of cells in S phase increased and cells in G1/G0 phase decreased. In conclusion, resveratrol can inhibit the proliferation of 4T1 cancer cells by inhibiting cell cycle and inducing apoptosis. Key Contribution:Resveratrol exerts a more obvious toxic effect on cancer cells to induce apoptosis in a time-and dose-dependent manner. RNA-seq was used to overall explore potential mechanisms from a transcriptional level.

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“…There is also a clear need for an established cell line in the schistosome molecular tool-kit and progress in this endeavor is being made, especially for Schistosoma japonicum (see review by Ye et al (2013)). Finally, the introduction here of the xCELLigence approach to analyze non-model, less conventional cell lines such as Bge of B. glabrata , is notable and predicts follow-up studies to examine host-parasite interaction in more detail such as in Transwell/co-culture experiments (Roshan Moniri et al, 2014). With the increasing availability of sequence information of the genome and transcriptome of both the snail host and the schistosome, it is opportune and timely to optimize Bge cells as companion ‘tools’ to provide more fundamental information on the schistosome/snail host-parasite relationship, vector biology and indeed novel interventions.…”

Section: Discussionmentioning

confidence: 99%

Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

Rinaldi

Yan

Nacif-Pimenta

et al. 2015

International Journal for Parasitology

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The invertebrate cell line, Bge, from embryos of the snail Biomphalaria glabrata, remains to date the only established cell line from any species of the Phylum Mollusca. Since its establishment in 1976 by Eder Hansen, few studies have focused on profiling its cytometrics, growth characteristics or sensitivity to xenobiotics. Bge cells are reputed to be challenging to propagate and maintain. Therefore, even though this cell line is a noteworthy resource, it has not been studied widely. With growing interest in functional genomics, including genetic transfection, to elucidate molecular aspects of the snail intermediate hosts responsible for transmission of schistosomiasis, and aiming to enhance the convenience of maintenance of this molluscan cell line, we deployed the xCELLigene real time approach to study Bge cells. Doubling times for three isolates of Bge, termed CB, SL and UK, were longer than for mammalian cell lines - longer than 40 h in complete Bge medium supplemented with 7% fetal bovine serum at 25°C, ranging from ∼42 h to ∼157 h when 40,000 cells were seeded. To assess the potential of the cells for genetic transformation, antibiotic selection was explored. Bge cells were sensitive to the aminonucleoside antibiotic puromycin (from Streptomyces alboniger) from 5 μg/ml to 200 ng/ml, displaying a half maximal inhibitory concentration (IC50) of ∼1.91 μg/ml. Sensitivity to puromycin, and a relatively quick kill time (<48 h in 5 μg/ml) facilitated use of this antibiotic, together with the cognate resistance gene (puromycin N-acetyl-transferase, PAC) for selection of Bge cells transformed with the PAC gene (puroR). Bge cells transfected with a plasmid encoding puroR were partially rescued when cultured in the presence of 5 μg/ml of puromycin. These findings pave the way for the development of functional genomic tools applied to the host-parasite interaction during schistosomiasis and neglected tropical trematodiases at large.

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Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA)

Cited by 112 publications

References 16 publications

Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells

Synergistic effects of combined treatment with histone deacetylase inhibitor suberoylanilide hydroxamic acid and TRAIL on human breast cancer cells

The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells

Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

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