Dynamic approaches of mixed species biofilm formation using modern technologies

Doiron, Kim; Linossier, Isabelle; Faÿ, Fabienne; Yong, Julius; Wahid, Effendy Abd; Hadjiev, D.; Bourgougnon, Nathalie

doi:10.1016/j.marenvres.2012.04.001

Cited by 19 publications

(12 citation statements)

References 40 publications

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“…This species is deeply studied for its extracellular polymeric substances, its adhesion mechanisms and its relationships with bacteria [ 36 , 37 , 38 ]. It is able to form mixed biofilm with bacterial strains and a possible role of bacterial signal molecules AHL in the formation of its biofilm has been indicated [ 39 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment

Norcy

Niemann

Proksch

et al. 2017

IJMS

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Dibromohemibastadin-1 (DBHB) is an already known potent inhibitor of blue mussel phenoloxidase (which is a key enzyme involved in bioadhesion). Within this study, the potentiality of DBHB against microfouling has been investigated. The activity of DBHB was evaluated on key strains of bacteria and microalgae involved in marine biofilm formation and bioassays assessing impact on growth, adhesion and biofilm formation were used. To assess the efficiency of DBHB when included in a matrix, DBHB varnish was prepared and the anti-microfouling activity of coatings was assessed. Both in vitro and in situ immersions were carried out. Confocal Laser Scanning Microscopy (CLSM) was principally used to determine the biovolume and average thickness of biofilms developed on the coatings. Results showed an evident efficiency of DBHB as compound and varnish to reduce the biofilm development. The mode of action seems to be based principally on a perturbation of biofilm formation rather than on a biocidal activity in the tested conditions.

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Section: Resultsmentioning

confidence: 99%

Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment

Norcy

Niemann

Proksch

et al. 2017

IJMS

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“…However, other researchers refrain from differentiating the processes of microcolony formation and biofilms maturation (Monds and O'Toole ). The structure of biofilms varies depending on bacterial types (Bridier and others ), age of biofilms (Doiron and others ; Xiao and others ), nutrients present on biotic or abiotic surfaces or the surrounding liquid media (Xiao and others ; Jahid and other ), and the species present in mixed‐species biofilms (Figure ; Stoodley and others ; Bridier and others ). The proportion of species present varies according to their propensity to form biofilms and, generally, the contribution of different species is unequal (Stoodley and others ).…”

Section: Reversible Attachmentmentioning

confidence: 99%

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Jahid

2014

Comp Rev Food Sci Food Safe

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Formation of mixed-species biofilms constitutes a common adaptation of foodborne pathogens and indigenous microbiota for prolonged survival in their food niche. Nevertheless, the potential role of mixed-species biofilms in food safety remains to be elucidated. The formation of mixed-species biofilms on food and food processing surfaces depends on various physical, chemical, and biological processes including species composition, especially of the indigenous microbiota and nutrients, food types, temperature, quorum sensing, extracellular polymeric substance (EPS) production, biofilms maturation, and dispersal steps. Compared to monospecies, mixed-species are highly resistant to antimicrobials, possibly due to higher EPS production, internalization into food, fitness of species, denser and thicker biofilms maturation, and interspecific protection of 1 species by others, although there are much debate among studies. The fitness of mixed-species biofilms populations is suggested to be of a cooperative, competitive, or neutral nature based on the genetic background of the involved species. Currently, various methods using microarray, confocal microscopy, proteomics, and selective media are being explored for the detection of mixed-species biofilms to resolve the conflict issues. Here, we review recent progress in this emerging field in the context of food safety and propose that novel and alternative techniques like antiquorum sensing, antibiofilms, enzymes, hurdle techniques, and bacteriophages will significantly help to control the formation of mixed-species biofilms for enhanced food safety. The next challenge will be to integrate the fitness and resistance patterns of mixed-species biofilms in the laboratory with those of natural settings.

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“…As it is known that biofilms are often dominated by bacterial communities, bacterial colonization dynamics of single or mixed bacterial strains have been intensively studied in laboratory experiments. In contrast, little is known about biofilm formation dynamics in natural environments (8)(9)(10)(11). In laboratory assays, it has been demonstrated that bacterial biofilm formation is a dynamic process with different phases of development.…”

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confidence: 99%

Fluorescence-Based Quasicontinuous and In Situ Monitoring of Biofilm Formation Dynamics in Natural Marine Environments

Fischer

Friedrichs²,

Lachnit

2014

Appl Environ Microbiol

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dAnalyzing the dynamics of biofilm formation helps to deepen our understanding of surface colonization in natural environments. While methods for screening biofilm formation in the laboratory are well established, studies in marine environments have so far been based upon destructive analysis of individual samples and provide only discontinuous snapshots of biofilm establishment. In order to explore the development of biofilm over time and under various biotic and abiotic conditions, we applied a recently developed optical biofilm sensor to quasicontinuously analyze marine biofilm dynamics in situ. Using this technique in combination with microscope-assisted imaging, we investigated biofilm formation from its beginning to mature multispecies biofilms. In contrast to laboratory studies on biofilm formation, a smooth transition from initial attachment to colony formation and exponential growth could not be observed in the marine environment. Instead, initial attachment was followed by an adaptation phase of low growth and homogeneously distributed solitary bacterial cells. Moreover, we observed a diurnal variation of biofilm signal intensity, suggesting a transient state of biofilm formation of bacteria. Overall, the biofilm formation dynamics could be modeled by three consecutive development stages attributed to initial bacterial attachment, bacterial growth, and attachment and growth of unicellular eukaryotic microorganisms. Additional experiments showed that the presence of seaweed considerably shortened the adaptation phase in comparison with that on control surfaces but yielded similar growth rates. The outlined examples highlight the advantages of a quasicontinuous in situ detection that enabled, for the first time, the exploration of the initial attachment phase and the diurnal variation during biofilm formation in natural ecosystems.

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Dynamic approaches of mixed species biofilm formation using modern technologies

Cited by 19 publications

References 40 publications

Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment

Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment

The Paradox of Mixed‐Species Biofilms in the Context of Food Safety

Fluorescence-Based Quasicontinuous and In Situ Monitoring of Biofilm Formation Dynamics in Natural Marine Environments

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