Dynamic and Responsive Growth Factor Delivery from Electrospun and Hydrogel Tissue Engineering Materials

Bruggeman, Kiara F.; Williams, Richard J.; Nisbet, David R.

doi:10.1002/adhm.201700836

Cited by 62 publications

(46 citation statements)

References 103 publications

(142 reference statements)

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“…In vitro, a cocktail of small molecules and proteins are supplemented into the culture media to promote neural induction and regional specification into lineage-restricted neuronal subpopulations such as midbrain dopaminergic or cortical progenitors. In vivo, numerous proteins such as neurotrophins, morphogens, chemokines and axon guidance molecules have been demonstrated to influence survival, differentiation and plasticity of new and residual stem cells (Chilton, 2006;Tayalia and Mooney, 2009;Moshayedi et al, 2016;Rodriguez et al, 2018;Bruggeman et al, 2018). However, even under optimal conditions, differentiations result in heterogeneous populations inclusive of proliferating progenitors, immature and mature neurons of the desired phenotype, as well as off-target populations.…”

Section: Utilizing Biomaterials To Deliver Trophic Support In Vitro Amentioning

confidence: 99%

Harnessing stem cells and biomaterials to promote neural repair

Bruggeman

Moriarty

Dowd

et al. 2018

British J Pharmacology

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With the limited capacity for self‐repair in the adult CNS, efforts to stimulate quiescent stem cell populations within discrete brain regions, as well as harness the potential of stem cell transplants, offer significant hope for neural repair. These new cells are capable of providing trophic cues to support residual host populations and/or replace those cells lost to the primary insult. However, issues with low‐level adult neurogenesis, cell survival, directed differentiation and inadequate reinnervation of host tissue have impeded the full potential of these therapeutic approaches and their clinical advancement. Biomaterials offer novel approaches to stimulate endogenous neurogenesis, as well as for the delivery and support of neural progenitor transplants, providing a tissue‐appropriate physical and trophic milieu for the newly integrating cells. In this review, we will discuss the various approaches by which bioengineered scaffolds may improve stem cell‐based therapies for repair of the CNS.

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Section: Utilizing Biomaterials To Deliver Trophic Support In Vitro Amentioning

confidence: 99%

Harnessing stem cells and biomaterials to promote neural repair

Bruggeman

Moriarty

Dowd

et al. 2018

British J Pharmacology

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“…The urgent task is to establish the influence of conditions of three-dimensional cultivation on the efficiency of survival, proliferation and potential for directed differentiation of multipotent stem cells of different origin. In addition to form-forming and supporting functions, matrixes can also regulate growth factors releasing during different time points providing not only cell proliferation but their direct differentiation in vivo as well [30,31]. It is shown that cell-cell interaction with extracellular basement of biomaterial and growth factors provide stem cell differentiation and tissue growth [32,33].…”

Section: а B Cmentioning

confidence: 99%

3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P

Кyryk¹,

Kuchuk²,

Mamchur³

et al. 2018

JCOT

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Actual issues during tissue regeneration are to ensure the survival of transplanted cells at the site of their application and further activity, especially in case of local pathological alterations such as inflammation and ischemia. For this purpose, the matrices that can not only fill the defects of tissues, but also be scaffolds for cells are developed.The aim of this study was to evaluate the effectiveness of 3D cultivation of murine adipose-derived multipotent mesenchymal stromal cells (MSMCs) in hydrogel based on carbomer 974P.Materials and methods. MSMCs were obtained from the adipose tissue of FVB-Cg-Tg(GFPU)5Nagy/J mice transgenic for GFP gene. The cells were phenotyped by flow cytometry and directly differentiated into osteogenic and adipogenic direction to confirm multipotent phenotype. MMSCs were cultured and directly differentiated into osteogenic direction in three-dimensional hydrogel scaffolds. For hydrogel preparation we used carbomer 974P with composition of glycerol, propylene glycol, triethylamine and agarose in original proportion.Results. The three-dimensional hydrogel based on carbomer 974P for the further engraftment with MMSCs was obtained. Modified protocols for the preparation of hydrogels based on carbomer and agarose and their rehydration by culture media for the 3D cultivation of adipose-derived MMSCs have been developed. The optimal concentration of MSMCs and the injection method for engraftment of hydrogels of the required form and size are selected. It was shown that adipose-derived MMSCs in 3D carbomer hydrogel preserve the potential of directed osteogenic differentiation.Conclusion. Three-dimensional hydrogel based on carbomer 974P is capable to support cells, provide the necessary cytoarchitectonics, maintain intercellular interactions, which can promote further long-term survival and specialization of graft.

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“…The latter often encompasses the inclusion of mechanical stimulation and/or biomolecules (e.g., growth factors, cytokines, etc.) for tailoring biomaterials to better recapitulate in vivo tissue microenvironments biomolecular signaling and mechanobiology . However, cells immobilization in preformed ECM‐mimetic supporting scaffolds is highly challenging, often resulting in low cell seeding density and heterogeneous spatial distribution .…”

Section: Introductionmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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Dynamic and Responsive Growth Factor Delivery from Electrospun and Hydrogel Tissue Engineering Materials

Cited by 62 publications

References 103 publications

Harnessing stem cells and biomaterials to promote neural repair

Harnessing stem cells and biomaterials to promote neural repair

3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P

Advanced Bottom‐Up Engineering of Living Architectures

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