Dynamic analysis of peripheral blood TCR β-chain CDR3 repertoire in occupational medicamentosa-like dermatitis due to trichloroethylene

Abstract

Previously, we had cross-sectionally explored the characteristics of T cell receptor (TCR) repertoires from occupational medicamentosa-like dermatitis due to trichloroethylene (OMDT) patients, now we further analyzed the dynamic features of OMDT TCR repertoires. Peripheral blood TCR β-chain complementarity-determining region 3 (CDR3) genes were detected with the high throughput sequencing in 24 OMDT cases in their acute, chronic and recovery stages, respectively, and in 24 trichloroethylene-exposed healthy controls. The TCR repertoire diversity, TRBV/TRBD/TRBJ gene usage and combination, frequencies of CDR3 nucleotide (nt) and amino acid (aa) sequences in the cases in different stages and in the controls were analyzed. TRBV6-4 and TRBV7-9 frequencies significantly differed between the cases and controls (both P < 6.1 × 10–4). TRBV6-4 combination with TRBJ2-1, TRBJ2-2, TRBJ2-3, and TRBJ2-6, and TRBV7-9 combination with TRBJ2-1 were associated with the stage by OMDT severity (all P < 0.001). Ten CDR3-nt and 7 CDR3-aa sequences in TRBV7-9-TRBJ2-1 combination and 1 CDR3-nt and 1 CDR3-aa sequences in TRBV6-4-TRBJ2-1 combination were identified as associated with the severity of OMDT (all P < 0.001). We revealed further how TCR repertoires vary with the severity in the development of OMDT, and severity-related TCRs may provide important therapeutic targets for OMDT in clinical practice.

Systematic Analysis of Tumor Microenvironment Patterns and Oxidative Stress Characteristics of Endometrial Carcinoma Mediated by 5-Methylcytosine Regulators

“…These genes can be used as potential biomarkers of endometrial cancer. The current research on these genes is limited, among which TRBJ2_3 is a kind of T cell TCR, which can regulate T cell function to a certain extent [34]; RP11_323D18.5 and RP11_405M12.2 were long-chain non-coding RNA related to m6A modification. Nevertheless, there are still some limitations to this research.…”

Establishment of a humanized mouse model using steady‐state peripheral blood‐derived hematopoietic stem and progenitor cells facilitates screening of cancer‐targeted T‐cell repertoires

“…It is well established that the third complementarity determining region (CDR3) of T-cell receptor (TCR) βchains (TRBs), the most variable region for antigen recognition and binding, is a vital structure to directly interact with the peptide presented by major histocompatibility complex and is the molecular basis for the recognition of cancer-specific antigens by T cells [7,8].…”

“…It is well established that the third complementarity determining region (CDR3) of T‐cell receptor (TCR) β‐chains (TRBs), the most variable region for antigen recognition and binding, is a vital structure to directly interact with the peptide presented by major histocompatibility complex and is the molecular basis for the recognition of cancer‐specific antigens by T cells [ 7 , 8 ]. Therefore, the effective functional screening of cancer‐targeted TRB CDR3 repertoires will contribute to identifying T cells with rearrangement sequences responding to specific cancers and will also benefit the discovery of specific cancer‐targeting T‐cell clones [ 9 , 10 ].…”