DWD HYPERSENSITIVE TO UV-B 1 is negatively involved in UV-B mediated cellular responses in Arabidopsis

Kim, Sang‐Hoon; Kim, Hani; Seo, Kyoung‐In; Kim, Soonhee; Chung, Sunglan; Huang, Xi; Yang, Po; Deng, Xing Wang; Lee, Jae‐Hoon

doi:10.1007/s11103-014-0247-0

Cited by 7 publications

(2 citation statements)

References 67 publications

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“…Another DWD protein, DWD HYPERSENSITIVE TO UV-B 1 (DHU1), also contributes to repression of the UV-B signal transduction pathway by interfering with COP1 function. DHU1 likely works in association with RUP1 and is able to bind DDB1, suggesting a role as a substrate adapter for CRL4 complexes (Kim et al, 2017; Kim et al, 2014).…”

Section: Nuclear Structure and Light As A Signalmentioning

confidence: 99%

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

Fonseca

Rubio

2019

Front. Plant Sci.

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CULLIN4 (CUL4) RING ligase (CRL4) complexes contain a CUL4 scaffold protein, associated to RBX1 and to DDB1 proteins and have traditionally been associated to protein degradation events. Through DDB1, these complexes can associate with numerous DCAF proteins, which directly interact with specific targets promoting their ubiquitination and subsequent degradation by the proteasome. A characteristic feature of the majority of DCAF proteins that associate with DDB1 is the presence of the DWD motif. DWD-containing proteins sum up to 85 in the plant model species Arabidopsis. In the last decade, numerous Arabidopsis DWD proteins have been studied and their molecular functions uncovered. Independently of whether their association with CRL4 has been confirmed or not, DWD proteins are often found as components of additional multimeric protein complexes that play key roles in essential nuclear events. For most of them, the significance of their complex partnership is still unexplored. Here, we summarize recent findings involving both confirmed and putative CRL4-associated DCAF proteins in regulating nuclei architecture remodelling, DNA damage repair, histone post-translational modification, mRNA processing and export, and ribosome biogenesis, that definitely have an impact in gene expression and de novo protein synthesis. We hypothesized that, by maintaining accurate levels of regulatory proteins through targeted degradation and transcriptional control, CRL4 complexes help to surveil nuclear processes essential for plant development and survival.

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Section: Nuclear Structure and Light As A Signalmentioning

confidence: 99%

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

Fonseca

Rubio

2019

Front. Plant Sci.

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“…Microarray analysis with biological replicates was performed based on a previous study by Kim et al (2014). Four-day-old Col-0 (control sample) and afa1-2 seedlings (test sample) grown with 0.25 μM ABA were used.…”

Section: Microarray Analysismentioning

confidence: 99%

Loss‐of‐function of ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination

Kim

Song

Kim

et al. 2021

Physiologia Plantarum

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ABA is a phytohormone involved in diverse plant events such as seed germination and drought response. An F‐box protein functions as a substrate receptor of the SCF complex and is responsible for ubiquitination of target proteins, triggering their subsequent degradation mediated by ubiquitin proteasome system. Here, we have isolated a gene named ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 (AFA1) that was upregulated by ABA. AFA1 interacted with adaptor proteins of the SCF complex, implying its role as a substrate receptor of the complex. Its loss of function mutants, afa1 seedlings, exhibited ABA‐hypersensitivity, including delayed germination in the presence of ABA. Moreover, loss of AFA1 led to increased drought tolerance in adult plants. Microarray data with ABA treatments indicated that 129 and 219 genes were upregulated or downregulated, respectively, by more than three times in afa1 relative to the wild type. Among the upregulated genes in afa1, the expression of 28.7% was induced by more than three times in the presence of ABA, while only 9.3% was repressed to the same extent. These data indicate that AFA1 is involved in the downregulation of many ABA‐inducible genes, in accordance with the ABA‐hypersensitive phenotype of afa1. Epistasis analysis showed that AFA1 could play a role upstream of ABI4 and ABI5 in the ABA signaling for germination inhibition. Collectively, our findings suggest that AFA1 is a novel F‐box protein that negatively regulates ABA signaling.

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Genome-wide identification, sequence characterization, and protein–protein interaction properties of DDB1 (damaged DNA binding protein-1)-binding WD40-repeat family members in Solanum lycopersicum

Zhu

Huang

Miao

et al. 2015

Planta

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Damaged DNA Binding 1 (DDB1)-binding WD40 (DWD) proteins are highly conserved and involved in a plethora of developmental and physiological processes such as flowering time control, photomorphogenesis, and abiotic stress responses. The phylogeny of this family of proteins in plants and algae of viridiplante is a critical area to understand the emergence of this family in such important and diverse functions. We aimed to investigate the putative homologs of DWD in the viridiplante and establish a deeper DWD evolutionary grasp. The advancement in publicly available genomic data allowed us to perform an extensive genome-wide DWD retrieval. Using annotated Arabidopsis thaliana DWDs as the reference , we generated and characterized a comprehensive DWD database for the studied photoautotrophs. Further, a generic DWD classification system (Type A to K), based on (i) position of DWD motifs, (ii) number of DWD motifs, and (iii) presence/absence of other domains, was adopted. About 72-80% DWDs have one DWD motif, whereas 17-24% DWDs have two and 0.5-4.7% DWDs have three DWD motifs. Neighbor-joining phyloge-netic construction of A. thaliana DWDs facilitated us to tune these substrate receptors into 15 groups. Though the DWD count increases from microalgae to higher land plants, the ratio of DWD to WD40 remained constant throughout the viridiplante. The DWD expansion appeared to be the consequence of consistent DWD genetic flow accompanied by several gene duplication events. The network, phylogenetic, and statistical analysis delineated DWD evolutionary relevance in the viridiplante.

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DWD HYPERSENSITIVE TO UV-B 1 is negatively involved in UV-B mediated cellular responses in Arabidopsis

Cited by 7 publications

References 67 publications

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

Loss‐of‐function of ARABIDOPSIS F‐BOX PROTEIN HYPERSENSITIVE TO ABA 1 enhances drought tolerance and delays germination

Genome-wide identification, sequence characterization, and protein–protein interaction properties of DDB1 (damaged DNA binding protein-1)-binding WD40-repeat family members in Solanum lycopersicum

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