Dwarf and short grain 1 , encoding a putative U-box protein regulates cell division and elongation in rice

Wang, Nan; Xing, Ya-Di; Lou, Qijin; Feng, Ping; Liu, Song; MeiDan, Zhu; Yin, Wuzhong; Fang, Shunran; Lin, Yan; Zhang, Tianquan; Sang, Xianchun; He, Guanghua

doi:10.1016/j.jplph.2016.11.012

Cited by 33 publications

(20 citation statements)

References 73 publications

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“…Meanwhile, we found that all PbrPUB genes contained the cis-acting related to hormone regulation, such as, abscisic acid (ABA), methyl jasmonate (MeJA), gibberellin (GA), salicylic acid, and auxin responsiveness elements. Previous study had reported that DSG1, which encodes a U-box domain, could regulate cell division and elongation by responding to multiple hormones, such as auxin, salicylic acid and ethylene [33]. In our study, 55 genes were identi ed as the responsiveness elements of ABA, suggesting that PUB gene family might particular in resistance under ABA treatment (Fig.…”

Section: Chromosomal Localization and Homologous Gene Analysis Of Pbrmentioning

confidence: 56%

Genome-Wide Identification and Functional Analysis of U-Box E3 Ubiquitin Ligases Gene Family Related to Drought Stress Response in Chinese white pear (Pyrus bretschneideri)

Wang

Song

Dai

et al. 2021

Preprint

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Background The plant U-box(PUB) proteins are a family of ubiquitin ligases (E3) enzymes that involved in diverse biological processes, as well as in responses to plant stress response. However, the characteristics and functional divergence of the PUB gene family has not yet been previously studied in the Chinese White Pear (Pyrus bretschneideri). Results In the present study, we identified 62 PbrPUBsin Chinese white pear genome. Based on the phylogenetic relationship, 62 PUB genes were clustered into five groups. The conserved motif and gene structure analysis provided further evidence to support the classification phylogenetic tree. The PbrPUB genes were unevenly distribution on 17 pear chromosomes, chromosome 15 housed most member of PUB family, with eight PUB genes. Cis-acting element analysis indicted PUB genes might participate in diverse biological processes, especially in the response to abiotic stresses. Based on RNA-data from “Dangshansuli” at seven tissues, we found that PUB genes exhibited diverse of expression level in seven tissues, and qRT-PCR experiment further support the reliable of RNA-Seq data. To identify candidate genes associated with resistance, we conducted qRT-PCR experiment the expression level of pear seed plant under four abiotic stresses, including: ABA, dehydration, salt and cold treatment. One candidate PUB gene associated with dehydration stress was selected to conduct further functional experiment. Subcellular localization revealed PbrPUB18 gene was located on cell nucleus. Furthermore, heterologous over-expression of PbrPUB18 in Arabidopsis indicated that the over-expression of PbrPUB18 could enhance resistance in drought treatment. In conclusions, we systematically identified the PUB genes in pear, and provided valuable information for the molecular mechanism of PUB genes in pear.

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Section: Chromosomal Localization and Homologous Gene Analysis Of Pbrmentioning

confidence: 56%

Genome-Wide Identification and Functional Analysis of U-Box E3 Ubiquitin Ligases Gene Family Related to Drought Stress Response in Chinese white pear (Pyrus bretschneideri)

Wang

Song

Dai

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Meanwhile, we found that all PbrPUB genes contained the cis -acting related to hormone regulation, such as salicylic acid, gibberellin (GA), auxin and methyl jasmonate (MeJA) responsiveness elements. Previous study had reported that DSG1 , which encodes a U-box domain, could regulate cell division and elongation by responding to multiple hormones, such as auxin, salicylic acid and ethylene [ 41 ]. ABA responsive element, named as ABRE, is one of most important cis -acting element in the promoter sequence of ABA-inducible genes response to ABA treatment [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification and functional analysis of U-box E3 ubiquitin ligases gene family related to drought stress response in Chinese white pear (Pyrus bretschneideri)

et al. 2021

View full text Add to dashboard Cite

Background The plant U-box (PUB) proteins are a family of ubiquitin ligases (E3) enzymes that involved in diverse biological processes, as well as in responses to plant stress response. However, the characteristics and functional divergence of the PUB gene family have not yet been previously studied in the Chinese white pear (Pyrus bretschneideri). Results In the present study, we identified 62 PbrPUBs in Chinese white pear genome. Based on the phylogenetic relationship, 62 PUB genes were clustered into five groups. The results of conserved motif and gene structure analysis supported the classification phylogenetic tree. The PbrPUB genes were unevenly distribution on 17 pear chromosomes, chromosome 15 housed most member of PUB family, with eight PUB genes. Cis-acting element analysis indicated that PUB genes might participate in diverse biological processes, especially in the response to abiotic stresses. Based on RNA-data from ‘Dangshansuli’ at seven tissues, we found that PUB genes exhibited diverse of expression level in seven tissues, and qRT-PCR experiment further supported the reliable of RNA-Seq data. To identify candidate genes associated with resistance, we conducted qRT-PCR experiment the expression level of pear seed plant under four abiotic stresses, including: ABA, dehydration, salt and cold treatment. One candidate PUB gene associated with dehydration stress was selected to conduct further functional experiment. Subcellular localization revealed PbrPUB18 protein was located on cell nucleus. Furthermore, heterologous over-expression of PbrPUB18 in Arabidopsis indicated that the over-expression of PbrPUB18 could enhance resistance in drought treatment. In conclusions, we systematically identified the PUB genes in pear, and provided useful knowledge for functional identification of PUB genes in pear.

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“…Different plant hormones interact each other hormones [75,76] and coordinately control the cell elongation, cell proliferation and cell differentiation [77]. Brassinosteroids (BR) related mutants display pleiotropic dwarf phenotypes caused by defects in cell elongation and differentiation processes, which determine the cell length [66,78]. In rice [79] and cotton [80], the mutation of GA biosynthesis related genes inhibits cell elongation, indicating that GA has an important role in internode cell elongation.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mapping and Candidate Gene Analysis for GA3 Responsive Short Internode in Watermelon (Citrullus lanatus)

Gebremeskel

Dou

et al. 2019

IJMS

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Plants with shorter internodes are suitable for high-density planting, lodging resistance and the preservation of land resources by improving yield per unit area. In this study, we identified a locus controlling the short internode trait in watermelon using Zhengzhouzigua (long internode) and Duan125 (short internode) as mapping parents. Genetic analysis indicated that F1 plants were consistent with long internode plants, which indicates that the long internode was dominant over the short internode. The observed F2 and BC1 individuals fitted the expected phenotypic segregation ratios of 3:1 and 1:1, respectively. The locus was mapped on chromosome 9 using a bulked segregant analysis approach. The region was narrowed down to 8.525 kb having only one putative gene, Cla015407, flanking by CAPS90 and CAPS91 markers, which encodes gibberellin 3β-hydroxylase (GA 3β-hydroxylase). The sequence alignment of the candidate gene between both parents revealed a 13 bp deletion in the short internode parent, which resulted in a truncated protein. Before GA3 application, significantly lower GA3 content and shorter cell length were obtained in the short internode plants. However, the highest GA3 content and significant increase in cell length were observed in the short internode plants after exogenous GA3 application. In the short internode plants, the expression level of the Cla015407 was threefold lower than the long internode plants in the stem tissue. In general, our results suggested that Cla015407 might be the candidate gene responsible for the short internode phenotype in watermelon and the phenotype is responsive to exogenous GA3 application.

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Dwarf and short grain 1 , encoding a putative U-box protein regulates cell division and elongation in rice

Cited by 33 publications

References 73 publications

Genome-Wide Identification and Functional Analysis of U-Box E3 Ubiquitin Ligases Gene Family Related to Drought Stress Response in Chinese white pear (Pyrus bretschneideri)

Genome-Wide Identification and Functional Analysis of U-Box E3 Ubiquitin Ligases Gene Family Related to Drought Stress Response in Chinese white pear (Pyrus bretschneideri)

Genome-wide identification and functional analysis of U-box E3 ubiquitin ligases gene family related to drought stress response in Chinese white pear (Pyrus bretschneideri)

Molecular Mapping and Candidate Gene Analysis for GA3 Responsive Short Internode in Watermelon (Citrullus lanatus)

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