Dusts causing pneumoconiosis generate OH and produce hemolysis by acting as fenton catalysts

Kennedy, Thomas P.; Dodson, Ronald F.; Rao, Narayanam V.; Ky, Henry; Hopkins, Chris; Baser, Michael E.; Tolley, Elizabeth A.; Hoidal, John R.

doi:10.1016/0003-9861(89)90118-5

Cited by 102 publications

(27 citation statements)

References 22 publications

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“…(7,8). For example, in studies using cell free systems, iron present on the surface of asbestos fibers can drive a modified Haber-Weiss (Fenton) reaction in which superoxide anion (0-)and hydrogen peroxide (H202) are converted to the highly toxic hydroxyl (-OH) radical (9)(10)(11). In general, the greater the iron content of the fiber, the more AOS generated by this process (12).…”

Section: Mechanisms Of Formation Of Active Oxygen Species By Asbestosmentioning

confidence: 99%

Oxygen Radicals and Asbestos-Mediated Disease

Quinlan¹,

Marsh²,

Janssen³

et al. 1994

Environmental Health Perspectives

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Asbestos fibers are potent elaborators of active oxygen species whether by reactions involving iron on the surface of the fiber, or by attempted phagocytosis of fibers by cell types resident in the lung. The link between production of active oxygen species and the pathogenesis of asbestosmediated disease has been highlighted by studies outlined here exploring the use of antioxidant scavengers which inhibit the cytotoxic effects of asbestos both in vitro and in vivo. The use of antioxidant enzymes ameliorates the induction of certain genes necessary for cell proliferation, such as ornithine decarboxylase, implicating oxidants as causative factors in some abnormal cell replicative events. Based on these observations, antioxidant enzymes likely represent an important lung defense mechanism in response to oxidative stress. In addition, their gene expression in lung or in cells from bronchoalveolar lavage might be a valuable biomarker of chronic inflammation and pulmonary disease after inhalation of oxidants. -Environ Health Perspect 102(Suppl 10): 107-110 (1994)

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Section: Mechanisms Of Formation Of Active Oxygen Species By Asbestosmentioning

confidence: 99%

Oxygen Radicals and Asbestos-Mediated Disease

Quinlan¹,

Marsh²,

Janssen³

et al. 1994

Environmental Health Perspectives

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“…Cells exposed to crocidolite pretreated with deferoxamine (an iron chelator) suffered substantially less cytotoxicity than cells exposed to untreated crocidolite, highlighting the role of iron in AOS generation (62,63). Treatment of silica with iron chelators also diminishes its oxidant generating capability (59).…”

Section: The Dismutasesmentioning

confidence: 99%

“…Rat alveolar macrophages and human neutrophils generated substantial AOS when exposed to silica (60) or asbestos (64), as determined by electron spin resonance and cytochrome c reduction. Addition of catalase to the incubation media more effectively inhibited AOS generation than did addition of SOD, implicating H202 and -OH as the major AOS produced by phagocytosis of these mineral dusts (59).…”

Section: The Dismutasesmentioning

confidence: 99%

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Regulation of Antioxidant Enzymes in Lung after Oxidant Injury

Quinlan¹,

Spivack²,

Mossman³

1994

Environmental Health Perspectives

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Studies have implicated active oxygen species lAOS) in the pathogenesis of various lung diseases. Many chemical and physical agents in the environment are potent generators of AOS, including ozone, hyperoxia, mineral dusts, paraquat, etc. These agents produce AOS by different mechanisms, but frequently the lung is the primary target of toxicity, and exposure results in damage to lung tissue to varying degrees. The lung has developed defenses to AOS-mediated damage, which include antioxidant enzymes, the superoxide dismutases [copper-zinc (CuZnSOD) and manganese-containing (MnSOD)I, catalase, and glutathione peroxidase (GPX). In this review, antioxidant defenses to environmental stresses in the lung as well as in isolated pulmonary cells following exposure to a number of different oxidants, are summarized. Each oxidant appears to induce a different pattern of antioxidant enzyme response in the lung, although some common trends, i.e., induction of MnSOD following oxidants inducing inflammation or pulmonary fibrosis, in responses to oxidants occur. Responses may vary between the different cell types in the lung as a function of cell-cycle or other factors. Increases in MnSOD mRNA or immunoreactive protein in response to certain oxidants may serve as a biomarker of AOSmediated damage in the lung. -Environ Health Perspect 102(Suppl 2): 79-87 (1994).

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“…Archer [15] reported that long fibers undergo incomplete uptake because of frustrated phagocytosis in lysosomes and enzyme release, leading to cytotoxicity. Hansen and Mossman [16] reported that the release of superoxide (O 2-) was increased in macrophages containing thin and long fibers with an aspect ratio of 3 or more, and there are many reports on the cytotoxicity of superoxide (O 2-) [17,18]. Watanabe et al [19,20] demonstrated that cell magnetometry showed a concentration-dependent delay of relaxation and a decrease in the relaxation coefficient after addition of PT and SiC to alveolar macrophages, demonstrating the cytotoxicity of PT and SiC.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and solubility evaluation of two types of whiskers by cell magnetometry

Kudo

Aizawa

2011

Environ Health Prev Med

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Objectives We investigated two types of whiskers, an antimony-containing tin-oxide-coated aluminum borate whisker (CABW) and an aluminum borate whisker (ABW), which are asbestos substitutes, in order to evaluate the safety of these fibers. Methods The cytotoxicity and solubility of CABW and ABW were evaluated by cell magnetometry, LDH assay and solubility test. Results ABW was found to be cytotoxic by cell magnetometry and slightly less soluble than CABW. In addition, it was found that the solubility of both fibers was intermediate between that of chrysotile and rock wool, as compared to our previous test results. Regarding the LDH assay, no significant difference was found among the fibers tested. These findings suggested that CABW, the surface of which is coated with antimony-containing tin oxide, had lower cytotoxicity and slightly higher solubility than ABW. Conclusions This study was only a short-term cytotoxicity and solubility study. Therefore, further safety assessment should be carried out in long-term experiments to examine the half-life of these fibers and monitor the potential development of lung carcinoma or mesothelioma after intratracheal instillation of these fibers in rats.

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Dusts causing pneumoconiosis generate OH and produce hemolysis by acting as fenton catalysts

Cited by 102 publications

References 22 publications

Oxygen Radicals and Asbestos-Mediated Disease

Oxygen Radicals and Asbestos-Mediated Disease

Regulation of Antioxidant Enzymes in Lung after Oxidant Injury

Cytotoxicity and solubility evaluation of two types of whiskers by cell magnetometry

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