DUS characterization and diversity assessment in pearl millet inbreds

Nehra, Mamta; Kumar, Mrinal; Vart, Dev; Sharma, Rajesh; Choudhary, Mukesh

doi:10.5958/0975-928x.2016.00126.5

Cited by 5 publications

(6 citation statements)

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“…The use of morphological descriptors for the assessment of genetic diversity and characterization of DUS criteria has been reported in inbred maize lines (Selvi et al 2013), moringa (Angadi and Jagadeesha 2018), pearl millet inbreds (Nehra et al 2016), and farmer rice variety (Rao et al 2013). The present study results proved the effectiveness of morphological characters for assessing genetic diversity and identifying DUS in sweet potato germplasm (Tairo et al 2018;Ochieng 2019).…”

Section: Discussionsupporting

confidence: 76%

“…Expression of morphological characteristics of leaf, vine/stem, and storage root are not much influenced by environmental factors; thus, they have been commonly used in assessing sweet potato diversity. Furthermore, morphological characters have also been used in the characterization of distinctness, uniformity, and stability (DUS) of plant genotypes for registration of new plant varieties and plant variety protection (PVP) of many crops (Selvi et al 2013;Rao et al 2013;Nehra et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity and characterization of distinctness, uniformity, and stability of newly bred sweet potato clones

Mau

Ndiwa²,

ARSA³

et al. 2022

Biodiversitas

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Abstract. Mau YS, Ndiwa ASS, Arsa IGBA, Asa GV, Nana A, Londingkene JA, Hosang EY, Kotta NRE. 2022. Assessment of genetic diversity and characterization of distinctness, uniformity, and stability of newly bred sweet potato clones. Biodiversitas 23: 5923-5934. Genetic diversity assessment is vitally important for germplasm management and the assembly of new varieties. This study aimed to 1) assess the genetic diversity and 2) characterize the distinctness, uniformity, and stability of newly bred clones and check varieties of sweet potatoes based on morphological characters. The present study was conducted in the experimental farm of Universitas Nusa Cendana for two cycles (years) in 2021 and 2022, involving 13 newly bred (hybrid) clones and 6 control varieties. The observations were made on morphological characters of leaf, vine, and storage root, including 31 characteristics. The observed characters were given scores based on the sweet potato descriptors of IPGBR and PPVTPP. The scored morphological data were subjected to cluster analysis followed by a Principal Component Analysis to reveal the genetic diversity level. Euclidean index was used to characterize the distinctness, while the variation of the scored morphological data was used to reveal the uniformity and stability of tested genotypes. The results revealed a high genetic diversity of the studied genotypes. The cluster analysis placed the studied genotypes into four clusters; cluster I consisted of only one genotype, cluster II comprised two sub-clusters and 13 genotype members, cluster II comprised two members, and cluster IV comprised two sub-clusters and three members. The first eight principal components were responsible for about 79% of the observed variability. Euclidean distance index revealed that each of the studied genotypes is distinct from others. No off-type plant was observed; thus, each of the tested genotypes was considered uniform. Phenotypic expressions of the studied genotypes were similar over the two growing cycles; thus, each is said to be stable. The newly bred genotypes G16 and G29 genotypes that meet the distinctness, uniformity, and stability criteria based on their highest number of distinctive characters are eligible for registration as new sweet potato varieties, more specifically for their unique purplish white and purple tuber flesh characters.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity and characterization of distinctness, uniformity, and stability of newly bred sweet potato clones

Mau

Ndiwa²,

ARSA³

et al. 2022

Biodiversitas

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“…Most of the germplasm accessions were tall (44) accessions while few were medium (6). Similar result was obtained by Nehra et al, (2016). The morphological descriptors are of immense help to the plant breeders for selecting desirable genotypes easily through visual assessment and further the highly contrasting germplasm accessions for a particular trait can be utilized to develop mapping populations in order to map QTLs.…”

Section: Plant Height At Maturity (Cm)supporting

confidence: 74%

DUS Characterization of Germplasm Accessions in Foxtail Millet (Setaria italica (L.) Beauv.)

Amarnath¹,

Prasad²,

Reddy³

2019

Int.J.Curr.Microbiol.App.Sci

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“…The leaf samples were collected from the Center of Excellence in Millets, Athiyandal. The genomic DNA was isolated from 15 days old seedlings by the modified CTAB method [5]. The genomic DNA quantity and quality was checked in Nanodrop and also using 0.8% agarose gel electrophoresis.…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Characterization of Little millet (Panicum sumatrense) varieties using Morphological descriptors and SSR based DNA fingerprinting

Senthil

Venugopalan

Selvamani

et al. 2020

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Little millet varieties are generally distinguished by morphological descriptors which are being used for seed certification and DUS characterization [1]. But in practical terms, these key differentiation descriptors between varieties of little millet are very fewer and hence difficult to differentiate germplasm accessions. Germplasm registration in NBPGR needs DNA fingerprint to show the uniqueness of germplasm in comparison to existing varieties. DNA fingerprinting is a better option to identify unique markers to differentiate the varieties. Available genomic resources are scarce since little millet is still considered to be an orphan crop. Therefore markers from other cereal genomes such as maize, pearl millet and barnyard millet that are been utilized for DNA fingerprinting purpose with a clue of cereal synteny relationship. Twenty-one morphological descriptors studies revealed that the variety ATL 1 is different from the other varieties for more than 16 morphological characters studied. DNA fingerprinting is attempted in five genotypes of little millets such as BL6, ATL 1, TNPsu 176, Co (Samai) 4, Paiyur 2 using cereal SSR markers. Among the 25 maize SSR markers used two markers viz., phi213984 and phi295450 scored polymorphism by the amplicon size of 310bp and 600bp respectively. From the 25 Pearl millet SSR markers used only one SSR marker found polymorphic at 305bp allele size for ATL 1 and Hence, SSR based DNA fingerprinting helped to differentiate ATL1, the newly released high yielding variety from other genotypes of little millets which can be used for varietal identification purpose.

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DUS characterization and diversity assessment in pearl millet inbreds

Cited by 5 publications

References 0 publications

Assessment of genetic diversity and characterization of distinctness, uniformity, and stability of newly bred sweet potato clones

Assessment of genetic diversity and characterization of distinctness, uniformity, and stability of newly bred sweet potato clones

DUS Characterization of Germplasm Accessions in Foxtail Millet (Setaria italica (L.) Beauv.)

Characterization of Little millet (Panicum sumatrense) varieties using Morphological descriptors and SSR based DNA fingerprinting

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