Durum wheat genes up-regulated in the early phases of cold stress are modulated by drought in a developmental and genotype dependent manner

Leonardis, Anna Maria De; Marone, Daniela; Mazzucotelli, Elisabetta; Neffar, Fahima; Rizza, Fulvia; Fonzo, N. di; Cattivelli, Luigi; Mastrangelo, Anna Maria

doi:10.1016/j.plantsci.2007.02.002

Cited by 41 publications

(34 citation statements)

References 56 publications

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“…Recent papers have demonstrated that the expression of genes involved in the water stress response is dependent on the developmental stage (De Leonardis et al. 2007) as is solute accumulation (Zhu et al.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Drought‐Related Traits and Screening Methods at Different Developmental Stages in Spring Barley

Szira

Bálint

Börner

et al. 2008

J Agronomy Crop Science

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Despite intensive research and breeding efforts, the physiological and quantitative genetic bases of drought tolerance are still poorly understood. The comparison of results obtained from different sources is also complex, because different testing methods may lead to controversial conclusions. This report discusses various drought stress experiments (hydroponics and in soil) in which the plant tolerance was studied at different developmental stages. Tests were performed in the germination, seedling and adult plant stages on the parental lines of five well‐known barley‐mapping populations. The results suggest that drought tolerance is a stage‐specific trait and changes during the life cycle. The effect of drought stress depended not only on the duration and intensity of water deficiency, but also on the developmental phase in which it began. To induce the same type of stress and to obtain comparable tolerance information from the replications, it is recommended that drought stress should be induced at the same growth stage. Correlations between the traits, commonly associated with improved drought resistance (high relative water content under stress, proline accumulation, osmoregulation) with stress tolerance indexes, are also presented, while the advantages and disadvantages of the most frequently used screening methods are discussed.

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Section: Discussionmentioning

confidence: 99%

“…However, comparative drought‐related studies, which compare and evaluate the commonly used methods, have not yet been published. The findings of a recent paper point out that the molecular responses of plants to stress events may change greatly during the life cycle and depend on the stress conditions (De Leonardis et al. 2007).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Drought‐Related Traits and Screening Methods at Different Developmental Stages in Spring Barley

Szira

Bálint

Börner

et al. 2008

J Agronomy Crop Science

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“…Studies carried out with different durum wheat genotypes demonstrated that the responsiveness to elevated [CO 2 ] (Aranjuelo et al, 2013), water stress (De Leonardis et al, 2007; Aprile et al, 2013; Habash et al, 2014), and the combination of both (Erice et al, 2014) is genotype specific. Moreover, the growth stage greatly influences the response of durum wheat to elevated [CO 2 ] (Aranjuelo et al, 2011; Vicente et al, 2015a) and drought (Liu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Interactive Effects of Elevated [CO2] and Water Stress on Physiological Traits and Gene Expression during Vegetative Growth in Four Durum Wheat Genotypes

et al. 2016

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The interaction of elevated [CO2] and water stress will have an effect on the adaptation of durum wheat to future climate scenarios. For the Mediterranean basin these scenarios include the rising occurrence of water stress during the first part of the crop cycle. In this study, we evaluated the interactive effects of elevated [CO2] and moderate to severe water stress during the first part of the growth cycle on physiological traits and gene expression in four modern durum wheat genotypes. Physiological data showed that elevated [CO2] promoted plant growth but reduced N content. This was related to a down-regulation of Rubisco and N assimilation genes and up-regulation of genes that take part in C-N remobilization, which might suggest a higher N efficiency. Water restriction limited the stimulation of plant biomass under elevated [CO2], especially at severe water stress, while stomatal conductance and carbon isotope signature revealed a water saving strategy. Transcript profiles under water stress suggested an inhibition of primary C fixation and N assimilation. Nevertheless, the interactive effects of elevated [CO2] and water stress depended on the genotype and the severity of the water stress, especially for the expression of drought stress-responsive genes such as dehydrins, catalase, and superoxide dismutase. The network analysis of physiological traits and transcript levels showed coordinated shifts between both categories of parameters and between C and N metabolism at the transcript level, indicating potential genes and traits that could be used as markers for early vigor in durum wheat under future climate change scenarios. Overall the results showed that greater plant growth was linked to an increase in N content and expression of N metabolism-related genes and down-regulation of genes related to the antioxidant system. The combination of elevated [CO2] and severe water stress was highly dependent on the genotypic variability, suggesting specific genotypic adaptation strategies to environmental conditions.

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“…10). Besides being overinduced by cold and dehydration in leaves of wheat plants at seedling stage (Mastrangelo et al, 2005), TdRF1 was previously shown to be induced by a controlled drought treatment very similar to environmental dry conditions of wheat cultivation (De Leonardis et al, 2007). This induction was seen in different tissues and at different developmental stages, including booting, seed development, and maturation.…”

Section: Discussionmentioning

confidence: 88%

Identification of a Protein Network Interacting with TdRF1, a Wheat RING Ubiquitin Ligase with a Protective Role against Cellular Dehydration

et al. 2011

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Plants exploit ubiquitination to modulate the proteome with the final aim to ensure environmental adaptation and developmental plasticity. Ubiquitination targets are specifically driven to degradation through the action of E3 ubiquitin ligases. Genetic analyses have indicated wide functions of ubiquitination in plant life; nevertheless, despite the large number of predicted E3s, only a few of them have been characterized so far, and only a few ubiquitination targets are known. In this work, we characterized durum wheat (Triticum durum) RING Finger1 (TdRF1) as a durum wheat nuclear ubiquitin ligase. Moreover, its barley (Hordeum vulgare) homolog was shown to protect cells from dehydration stress. A protein network interacting with TdRF1 has been defined. The transcription factor WHEAT BEL1-TYPE HOMEODOMAIN1 (WBLH1) was degraded in a TdRF1-dependent manner through the 26S proteasome in vivo, the mitogen-activated protein kinase TdWNK5 [for Triticum durum WITH NO LYSINE (K)5] was able to phosphorylate TdRF1 in vitro, and the RING-finger protein WHEAT VIVIPAROUS-INTERACTING PROTEIN2 (WVIP2) was shown to have a strong E3 ligase activity. The genes coding for the TdRF1 interactors were all responsive to cold and/or dehydration stress, and a negative regulative function in dehydration tolerance was observed for the barley homolog of WVIP2. A role in the control of plant development was previously known, or predictable based on homology, for wheat BEL1-type homeodomain1(WBLH1). Thus, TdRF1 E3 ligase might act regulating the response to abiotic stress and remodeling plant development in response to environmental constraints.Plants are sessile organisms that must cope with their surrounding environments; thus, stresses such as drought, soil salinity, or extreme temperatures represent common events during their life cycle. These unfavorable conditions limit plant growth and development and strongly threat crop productivity, preventing the expression of the full genetic potential of cultivars. At the molecular level, upon plant exposition to stress, a plethora of genes are up-or down-regulated to reorganize the metabolic fluxes leading to plant adaptation as a final result. These responses provide plants with the capacity to avoid or mitigate the effects of stresses. Besides gene transcriptional changes, cells have evolved other types of molecular responses, such as posttranslational modifications of key proteins (Mazzucotelli et al., 2008). Among them, protein phosphorylation and ubiquitination are key components of the molecular response to different types of abiotic stresses. These modifications are implicated in signal transduction, positive and negative regulation of the response, and cross-connections between different signaling pathways and stress responses. The rapid and precise activation or inhibition of regulatory proteins is essential for the coordination of an array of physiological responses to cope with different stresses and to ensure a tightly controlled regulation of cellular changes induced by stress inte...

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Durum wheat genes up-regulated in the early phases of cold stress are modulated by drought in a developmental and genotype dependent manner

Cited by 41 publications

References 56 publications

Evaluation of Drought‐Related Traits and Screening Methods at Different Developmental Stages in Spring Barley

Evaluation of Drought‐Related Traits and Screening Methods at Different Developmental Stages in Spring Barley

Interactive Effects of Elevated [CO2] and Water Stress on Physiological Traits and Gene Expression during Vegetative Growth in Four Durum Wheat Genotypes

Identification of a Protein Network Interacting with TdRF1, a Wheat RING Ubiquitin Ligase with a Protective Role against Cellular Dehydration

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