Duration of the seminiferous epithelium cycle in the frugivorous bat Artibeus lituratus

Silva, Soraia Fonseca Marinho da; Vieira, Maria Eduarda de Lima; Freitas, Mariella Bontempo; Matta, Sérgio Luís Pinto da; Morais, Danielle Barbosa

doi:10.1016/j.theriogenology.2019.10.010

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…ecautada , as well as in other bats and mammals, the SEC is divided into eight stages, as described by Berndston [18]. Stage 2 was the most frequent, while in other species, stage 1 is usually the most observed [14, 15, 19, 33, 35, 40, 41]. Zygotene primary spermatocytes emerged only from stage 2, similarly to the observed in most mammals already studied.…”

Section: Discussionmentioning

confidence: 61%

“…However, it differs from the observed in other bat species, such as insectivore Molossus molossus and frugivore Sturnira lilium , in which these cells were found at stage 1 [15, 19, 32, 42]. The pachytene primary spermatocyte is found at all stages, since this phase may last for hours, days or even weeks, depending on the species [14, 19, 38, 39, 41].…”

Section: Discussionmentioning

confidence: 64%

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Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Silva

Dias

et al. 2019

PLoS ONE

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Diphylla ecaudata is a hematophagous bat endemic of South America, with food preference for bird blood. Given the lack of information about the reproductive activity of this species, this study aimed to describe the testicular morphology and histomorphometry of D. ecaudata in order to understand its reproductive biology, specially spermatogenesis. The animals were collected in Lajes city, Rio Grande do Norte, Brazil. Following euthanasia, the testes were histologically processed for morphological, morphometric, ultrastructural and immunohistochemical analyses. Their average body weight was 24.64g, with a gonadosomatic index of 0.49%, tubulesomatic index of 0.47%, and a total of 32.20m of seminiferous tubules per gram of testis. The pre-meiotic, meiotic, and post-meiotic phases accounted for 56.20%, 9.30%, and 34.50% of the seminiferous epithelium cycle, respectively. The ultrastructure of spermiogenesis was similar to that described in other mammals and the perforatorium was not observed in the sperm. Androgen receptors were detected in Sertoli cell nuclei and Leydig cell cytoplasm, while aromatase enzyme was detected only in Sertoli cell nuclei. FGF2 and BCL-2 activities were detected in the cytoplasm of zygotene and pachytene primary spermatocytes, as well as round and elongated spermatids. D. ecaudata showed testicular pattern similar to other mammals and characteristics common to other bat species. This species stood out for its high efficiency of Sertoli cells, which presented high capacity to support germ cells, besides the highest sperm production rates among those already recorded. This study is the first step towards the knowledge of D. ecaudata reproduction and the first description of its spermatogenesis.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 64%

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Silva

Dias

et al. 2019

PLoS ONE

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“…However, the results are controversial. For example, some researchers have reported that SARS-CoV-2 was not detected in the male reproductive tract, [25][26][27][28][29][30][31][32][33][34] while others reported that SARS-CoV-2 RNA was found in the semen or testes of COVID-19 patients. 35,36 There are also unknown factors regarding COVID-19 and male reproduction.…”

Section: Introductionmentioning

confidence: 99%

Spermatogenesis in the Roborovski hamster (Phodopus roborovskii) and the Chinese hamster (Cricetulus griseus)

2021

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Background: Spermatogenesis is an elaborately organized and tightly regulated differentiation process. The spermatogenesis duration is stable within a certain species but highly variable between species of the same family.Objectives: In this study, the spermatogenesis duration of the Roborovski hamster was measured for the first time, and the spermatogenesis duration of the Chinese hamster was re-assessed. Materials and methods:Stage classification and cycle length measurement were carried out by labeling the dividing cells with bromodeoxyuridine and an antibody-based chromogen as well as with the periodic acid-Schiff/hematoxylin stain. Analysis was conducted using reference calculation and linear regression. Morphological measurements completed our set of methods. Results:The mean duration of one seminiferous epithelium cycle was 8.58 ± 0.34 days (mean ± SEM; Phodopus roborovskii) and 16.59 ± 0.47 days (Cricetulus griseus) based on the reference calculation. Slightly higher results were obtained using linear regression analysis: 9.72 ± 0.41 days for P. roborovskii and 17.64 ± 0.61 days for C. griseus.Additionally, a newly developed exemplary flowchart was proposed for the Roborovski hamster to facilitate spermatogenesis stage classification also in other species. The Chinese hamster presented an unexpectedly high paired epididymides weight of 1.701 ± 0.046 g (mean ± SEM) although having a body weight of only 40.5 ± 0.7 g. However, no significant correlation between the relative epididymis weight and spermatogenesis duration in mammals (Spearman rank correlation: r = −0.119, p = 0.607, n = 21) or rodents could be found (r = 0.045, p = 0.903, n = 11). Conclusion:Our data emphasize the stability of the spermatogenesis duration within species and its remarkable variability between species. Further research is needed to identify the principal mechanisms and selection drivers that are responsible for such stability within species and the variability between species.

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Hormones and reproductive cycles in bats

Krishna,

Roy,

Banerjee

2024

Hormones and Reproduction of Vertebrates

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Duration of the seminiferous epithelium cycle in the frugivorous bat Artibeus lituratus

Cited by 4 publications

References 18 publications

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Spermatogenesis in the Roborovski hamster (Phodopus roborovskii) and the Chinese hamster (Cricetulus griseus)

Hormones and reproductive cycles in bats

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