Duration of Storage Reduced Erythrocytes Profiles and Plasmodium Viability in Donor Blood

Abstract

Background Malaria screening for blood derived from any donors prior to transfusions is a standard procedure that should be performed; but, in fact, it is not routinely conducted. In case of the blood is infected with Plasmodium spp., the survival of parasites may be depending on, or even influencing, the profile of red blood cells (RBCs). Methods This observational longitudinal study was conducted upon 55 bags of donor blood that randomly selected. Malaria infections were detected using Rapid Diagnostic Test/RDT with thin and thick blood smear confirmation. The changes of Plasmodium spp. viability and RBCs profiles, as well as other hematological parameters, were observed from the results of routine hematological examinations which were performed on days 1,7,14 and 21 of storage. Results Among 55 blood samples, there were 17 and 38 bags, respectively, positive and negative for malaria, then used for analysis as the case and control groups. There were significant decreasing values (p<0.05) of all routine blood examination parameters of donor blood, started from days 1, 7, 14, 21, and 28. There were no differences in decreasing profiles between those infected and non-infected donor blood (p>0.05). On days 21 and 28 none of the positive samples still contained parasites. Conclusion Erythrocytes profiles of donor blood significantly decreased with the duration of storage, but were not influenced by the presence of Plasmodium spp.

Pengaruh Suhu Penyimpanan Terhadap Jumlah Eritrosit Pada Transfusi Darah di Rumah Sakit Bank Darah RSUD Dr. Soedarso Pontianak

“…Penambahan garam dan manitol menurunkan kadar hemolisis, dan glukosa menyediakan jalur substrat energi dan adenin mempertahankan kadar ATP. 13,14 Penelitian ini terdiri dari 3 kelompok perlakuan yaitu suhu 2 0 C, suhu 4 0 C dan 6 0 C dengan penentuan jumlah sampel diambil dari rumus (t-1) (r-1) ≥ 15 sehingga didapat 9 sampel sehingga diperoleh sampel sebanyak 27 sampel.…”