Duration of protective immunity induced by Mycoplasma gallisepticum strain ts-304 vaccine in chickens

Transcriptomic Analysis of Long-Term Protective Immunity Induced by Vaccination With Mycoplasma gallisepticum Strain ts-304

“…Therefore, development of vaccines with improved characteristics is necessary to further reduce the impact of losses associated with M. gallisepticum in poultry. The findings in this study and our recently reported duration of immunity study (29) have shown that the pathological changes in the tracheal mucosa caused by infection with virulent M. gallisepticum are not detectable in birds challenged 57 weeks after vaccination with strain ts-304, demonstrating that the protection induced by this new vaccine maintains the structural and functional integrity of the tracheal mucosa in the face of challenge with an aerosol of virulent M. gallisepticum. Assessment of the global transcriptional profiles of the tracheal mucosae of vaccinated-and-challenged birds and comparisons with uninfected birds demonstrated that they had completely recovered from any effects of infection on gene transcription within 2 weeks.…”

“…The procedures used for experimental vaccination and infection with virulent M. gallisepticum have been described previously (29). Briefly, 20 White Leghorn specific-pathogen-free (SPF) chickens were randomly allocated into two different groups of 10 (Groups 1 and 2) and inoculated by eye-drop with 30 ml ts-304 vaccine containing 10 6.0 color changing units (CCU) of strain ts-304 at 3 weeks of age and kept in microbiologically secure isolators until they were 60 weeks of age.…”

“…A previous study by our laboratory examined the protective immunity afforded by vaccination with strain ts-304 against chronic M. gallisepticum infection, but only at four weeks after vaccination (28). In a recent study we examined the duration of immunity induced by vaccination with ts-304 and found that it persisted for at least 57 weeks after a single vaccination at 3 weeks of age (29).…”

Mucosal immune responses in the trachea after chronic infection withMycoplasma gallisepticumin unvaccinated and vaccinated mature chickens

“…These B cells are likely to be IgG (or more correctly, IgY) (Leslie & Clem, 1969;Shimizu et al, 1992) or IgM secreting cells, as mucosal IgA secretion is T cell dependent in chickens (Lawrence et al, 1979). We have detected significantly higher concentrations of serum IgG in these chickens 40-, 48-and 57-weeks after vaccination than at 2 weeks after vaccination (Kanci Condello, Kulappu Arachchige, et al, 2020). These findings suggest that the antigenspecific antibodies either transudated into or locally produced in the trachea by birds vaccinated with the ts-304 vaccine may prevent infection by blocking attachment of pathogen to the respiratory epithelium, as has been suggested previously (Avakian & Ley, 1993;Javed et al, 2005).…”

Prudent Use of Tylosin for Treatment of Mycoplasma gallisepticum Based on Its Clinical Breakpoint and Lung Microbiota Shift

“…MG mainly infects turkeys and chickens, but it has also been reported in other birds ( Sawicka et al, 2020 ); it can be transmitted horizontally through direct or indirect contact or vertically through eggs ( Kleven, 2008 ). Although vaccination is effective for MG at present ( Bennett et al, 2013 ; Condello et al, 2020 ; Arachchige et al, 2021 ), vaccination against MG is not prevalent due to the variation of bacterial strains and the lack of means to prevent horizontal infection ( Feberwee et al, 2006 ).…”