Duration of protection and humoral immunity induced by an adenovirus-vectored subunit vaccine for foot-and-mouth disease (FMD) in Holstein steers

Sitt, Tatjana; Kenney, Mary Alice; Barrera, José; Pandya, Mital; Eckstrom, Korin; Warner, Megan; Pacheco, Juan M.; LaRocco, Michael; Palarea‐Albaladejo, Javier; Brake, David A.; Rieder, Elizabeth; Arzt, Jonathan; Barlow, John W.; Golde, William T.

doi:10.1016/j.vaccine.2019.08.017

Cited by 12 publications

(13 citation statements)

References 20 publications

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“…The duration of the protective immunity conferred by dendrimer B 2 T was close to those elicited by conventional vaccines (Robinson et al, 2016) and that recently reported for cattle immunized with a deficient human adenovirus vector expressing FMDV empty capsids (Sitt et al, 2019). 435 1,437 1,447 1,445 1,449 1, for 1 year after revaccination, performed 6 months after the first dose (Smitsaart & Bergmann, 2017).…”

Section: Discussionsupporting

confidence: 79%

“…The duration of the protective immunity conferred by dendrimer B 2 T was close to those elicited by conventional vaccines (Robinson et al, ) and that recently reported for cattle immunized with a deficient human adenovirus vector expressing FMDV empty capsids (Sitt et al, ). Indeed, in some FMD‐endemic regions, for example South America, manufacturers are required to show that oil‐adjuvanted conventional vaccines are able to induce protective antibody levels for 180 days after one dose and for 1 year after revaccination, performed 6 months after the first dose (Smitsaart & Bergmann, ).…”

Section: Discussionsupporting

confidence: 78%

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A bivalent B‐cell epitope dendrimer peptide can confer long‐lasting immunity in swine against foot‐and‐mouth disease

Cañas‐Arranz

Forner

Defaus

et al. 2020

Transbound Emerg Dis

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Development of safe, cost-effective vaccines is a desirable goal in practically all areas of human and animal health. In the latter field, foot-and-mouth disease (FMD) is paradigmatic of the challenge of designing strategies alternative to the conventional vaccines still used to prevent this highly transmissible, devastating livestock disease (Grubman & Baxt, 2004). Classical FMD vaccines rely on chemically inactivated FMD virus (FMDV) administered with an adjuvant and require about 7 days to induce a protective immunity that requires re-vaccination after 6-12 months to maintain its protective effect (Doel, 2005; Rodriguez & Grubman, 2009). High levels of circulating neutralizing antibodies are considered as the main correlate for FMDV protection, albeit this relationship is not absolute and differences in resistance to virus challenge can be found in animals with similar levels of neutralizing antibodies (McCullough et al., 1992). Duration of the protective immunity is essential in establishing the fitness of any FMD vaccine (Smitsaart & Bergmann, 2017), and boosting at approximately 6 monthly intervals is often required to maintain a protective response in pigs (Cox,

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 78%

A bivalent B‐cell epitope dendrimer peptide can confer long‐lasting immunity in swine against foot‐and‐mouth disease

Cañas‐Arranz

Forner

Defaus

et al. 2020

Transbound Emerg Dis

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“…This increment of VN antibodies in protected animals, matching that of a non-immunized pig, might reflect virus multiplication to a limited extent not resulting in detectable viremia (Table 2). Similar levels of VN antibodies boost upon virus challenge have been reported in pigs and cattle immunized with live-vectored vaccines or FMDV-like particles [34,35].…”

Section: Discussionsupporting

confidence: 72%

A Single Dose of Dendrimer B2T Peptide Vaccine Partially Protects Pigs against Foot-and-Mouth Disease Virus Infection

et al. 2020

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Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals whose control relies on efficient vaccination. We have reported that dendrimer peptide B2T, with two copies of FMDV B-cell epitope VP1 (136–154) linked through maleimide units to T-cell epitope 3A (21–35)], elicits potent B- and T-cell specific responses and confers solid protection in pigs to type-O FMDV challenge after two doses of peptide. Herein we now show that B2T evokes specific protective immune responses after administration of a single dose of either 2 or 0.5 mg of peptide. High titers of ELISA and neutralizing antibodies against FMDV were detectable at day 15 post-immunization. Likewise, activated T cells and induced IFN-γ response to in vitro recall with FMDV peptides were also detected by the same day. Further, in 70% of B2T-vaccinated pigs, full protection—no clinical signs of disease—was observed upon virus challenge at day 25 post-immunization. These results strengthen the potential of B2T as a safe, cost-effective candidate vaccine conferring adequate protection against FMDV with a single dose. The finding is particularly relevant to emergency scenarios permitting only a single shot immunization.

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“…One was the inactivated antigen against PRRS virus, and the other antigen selected was the FMD VLPs. Both PRRS and FMD are the highly contagious diseases of great economic significance in the livestock industry worldwide, which highlights the importance of targeted vaccine development [23, 24]. Their most commercial vaccines contain oil‐based adjuvants [25, 26].…”

Section: Resultsmentioning

confidence: 99%

Green preparation of hydrogel particles‐in‐emulsions for simultaneous enhancement of humoral and cell‐mediated immunity

Zou

Ngai

et al. 2020

Engineering in Life Sciences

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Emulsions are one of the most often used vaccine adjuvant formulations. Although they promote high humoral immunity, the induced cellular immunity is often poor, which restrict their application. To enhance the cellular immunity, some researchers have prepared mixed formulations by adding particles into the aqueous phase of emulsions. However, the particle preparation process usually involves the addition and removal of organic reagents, which is environmentally unfriendly and cumbersome. Moreover, the obtained vaccine adjuvant only induces limited cell‐mediated immunity and humoral immunity compared with emulsion‐adjuvanted vaccines. Herein, we developed a green and simple method for fabricating a novel nanoparticles‐in‐emulsions (NPE) formulation. Firstly, a temperature‐sensitive hydrogel was used to prepare particles by self‐solidification without additional crosslinking reagents. Secondly, the white oil was used as organic phase to avoid the particle washing procedures and organic solvent residues. Moreover, the effect of NPE as vaccine adjuvant was evaluated by using two veterinary vaccines as model antigens. NPE showed advantages than the conventional vaccine formulations in inducing both humoral and cellular immunity. This work provides a facile and broadly applicable approach for preparing nanoparticles‐in‐emulsions formulation, and presents an effective adjuvant for enhancing immunity against infectious diseases.

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Duration of protection and humoral immunity induced by an adenovirus-vectored subunit vaccine for foot-and-mouth disease (FMD) in Holstein steers

Cited by 12 publications

References 20 publications

A bivalent B‐cell epitope dendrimer peptide can confer long‐lasting immunity in swine against foot‐and‐mouth disease

A bivalent B‐cell epitope dendrimer peptide can confer long‐lasting immunity in swine against foot‐and‐mouth disease

A Single Dose of Dendrimer B2T Peptide Vaccine Partially Protects Pigs against Foot-and-Mouth Disease Virus Infection

Green preparation of hydrogel particles‐in‐emulsions for simultaneous enhancement of humoral and cell‐mediated immunity

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