Durable nanofibrous matrices augmented with hydrotalcite-like compounds for cutaneous regeneration of burn wounds

Devi, Mohan Vimala; Sobhana, S. S. Liji; Shiny, Punalur John; Ramanathan, Giriprasath; Felciya, Sekar Jeyakumar Grace; Poornima, V.; Thennarasu, Sathiah; Fardim, Pedro; Uma, T.

doi:10.1016/j.clay.2020.105476

Cited by 9 publications

(13 citation statements)

References 64 publications

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“…The hemocompatibility of the latex was intended to be the essential assay while using the latex-loaded nanofibrous matrix at the wound site for the regeneration. The extracted latex exhibited no visible sign of hemolysis when compared to the positive control . Moreover, according to the standards, samples with less than 5% are considered hemocompatible that can be evident from the Figure .…”

Section: Resultsmentioning

confidence: 99%

“…Nonetheless, the synergistic effect of CA and L in the nanofibrous matrix significantly enhances the strength of the bilayer wound-dressing material. 33 Normally, dressing were changed for the open wound periodically at an interval of 4 days, 32 but given the material stability, they can be altered within a 5-day 11 period. However, the CA:L-CSPG bilayer matrix exhibited with 43.83% of degradation will favor as a suitable dressing material for wound healing application.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the latex incorporated bilayer matrix act as dressing material topically with excellent hemocompatibility with the blood cells. 32 , 66 …”

Section: Resultsmentioning

confidence: 99%

“…Hemolytic percentage is calculated using the following formula where A S is the absorbance of test sample and A PC and A NC are the absorbance values of positive and negative controls, respectively. 32 …”

Section: Materials and Methodsmentioning

confidence: 99%

“…After incubation, the samples were centrifuged at 5000 rpm for 10 min and the absorbance of the supernatants was read at 540 nm. Hemolytic percentage is calculated using the following formula where A S is the absorbance of test sample and A PC and A NC are the absorbance values of positive and negative controls, respectively …”

Section: Materials and Methodsmentioning

confidence: 99%

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Fabrication of Biohybrid Cellulose Acetate-Collagen Bilayer Matrices as Nanofibrous Spongy Dressing Material for Wound-Healing Application

et al. 2020

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Tissue engineering is currently one the fastest growing engineering fields, requiring fabrication of advanced and multifunctional materials to be used as scaffolds or dressing for tissue regeneration. In this work, a bilayer matrix was fabricated by electrospinning of a hybrid cellulose acetate nanofibers (CA) containing bioactive latex or Ciprofloxacin over highly interconnected collagen (CSPG) 3D matrix previously obtained by a freeze-drying process. The bilayer matrix was fabricated with a nanofibrous part as the primary (top) layer and a spongy porous part as the secondary (bottom) layer by combining electrospinning and freeze-drying techniques to enhance the synergistic effect of both materials corresponding to physical and biological properties. The final material was physicochemically characterized using Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The bilayer matrix exhibited nanofibrous and 3D porous structure with properties such as high porosity, swelling, and stability required for soft-tissue-engineering applications. Furthermore, the in vitro biological and fluorescence properties of the matrix were tested against NIH 3T3 fibroblast and human keratinocyte (HaCaT) cell lines and showed good cell adhesion and proliferation over the bilayer matrix. Thus, the synergistic combination of nanofibrous material deposition onto to the collagenous porous material has proved efficient in the fabrication of a bilayer matrix for skin-tissue-engineering applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Moreover, the latex incorporated bilayer matrix act as dressing material topically with excellent hemocompatibility with the blood cells. 32 , 66 …”

Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

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Fabrication of Biohybrid Cellulose Acetate-Collagen Bilayer Matrices as Nanofibrous Spongy Dressing Material for Wound-Healing Application

et al. 2020

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Multifunctional Nanoclay‐Based Hemostatic Materials for Wound Healing: A Review

Tan,

Yang,

Zheng

et al. 2023

Adv Healthcare Materials

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Bleeding to death accounts for around 30–40% of all trauma‐related fatalities. Current hemostatic materials are mainly mono‐functional or have insufficient hemostatic capacity. Nanoclay has been recently shown to accelerate hemostasis, improve wound healing, and provide the resulting multifunctional hemostatic materials antibacterial, anti‐inflammatory, and healing‐promoting due to its distinctive morphological structure and physicochemical properties. Herein, the chemical design and action mechanism of nanoclay‐based hemostatic, antibacterial, and pro‐wound healing materials in the context of wound healing are discussed. The physiological processes of hemostasis and wound healing to elucidate the significance of nanoclay for functional wound hemostatic dressing design are outlined. A summary of the features of various nanoclay and product types used in wound hemostatic dressings is provided. Nanoclay can be antimicrobial due to the slow release of metal ions and has an abundant surface charge allowing for high affinity for proteins and cells, which can activate the coagulation reaction or facilitate tissue repair. Nanoclay with a microporous structure can be used as drug carriers to create composites critical for inhibiting bacterial growth on wounds or promoting the regeneration of vascular, muscle, and skin tissues. Directions for further research and innovation of nanoclay‐based multifunctional materials for hemostasis and tissue regeneration are explored.

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Resorbable Layered Double Hydroxides‐Based Composite Implants Synergistically Accelerates Critical‐Sized Defect Closure

Mohan,

V. K.,

Haridoss

et al. 2023

Advanced Therapeutics

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The study focuses on the fabrication of a composite implant composed of MgAl Layered Double Hydroxides (LDH), gelatin and diosgenin and its regeneration potential in experimentally‐induced tibial defect in rabbits. The implant was fabricated by blending and lyophilization technique and eventually cross‐linked using glutaraldehyde. The implant was characterized by Attenuated Total Reflectance (ATR) spectroscopy, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and compressive strength to analyse the morphological characteristics of the implant. A sustained release of diosgenin occurred over a period of 28 days. The osteogenic activity of the implant was analysed by alkaline phosphatase activity and Alizarin Red S staining; that aids in determining the extent of biomineralization. The results of the present study also demonstrated that LGD100 implant stimulates the expression of genes associated with osteoblastic activities in MG‐63 cells, namely type I collagen (COL I), osteocalcin (OCN) and osteopontin (OPN). We then tested the bone regeneration capacity of diosgenin‐loaded gelatin‐based LDH implant by creating critical bone defects in the tibia of rabbits by using empty bone defects as controls. Periodic radiographs were taken which showed promising results. Histological and immunohistochemical staining confirmed that after treatment with LGD100, new bone was formed at the defect site. This study suggests that LGD100 implant aids in faster bone formation as it mimics the natural properties of bone.This article is protected by copyright. All rights reserved

show abstract

Durable nanofibrous matrices augmented with hydrotalcite-like compounds for cutaneous regeneration of burn wounds

Cited by 9 publications

References 64 publications

Fabrication of Biohybrid Cellulose Acetate-Collagen Bilayer Matrices as Nanofibrous Spongy Dressing Material for Wound-Healing Application

Fabrication of Biohybrid Cellulose Acetate-Collagen Bilayer Matrices as Nanofibrous Spongy Dressing Material for Wound-Healing Application

Multifunctional Nanoclay‐Based Hemostatic Materials for Wound Healing: A Review

Resorbable Layered Double Hydroxides‐Based Composite Implants Synergistically Accelerates Critical‐Sized Defect Closure

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