The moth-eye structure is an anti-reflection structure that is expected to effective over a wide range of wavelengths and angles of incident light. In addition, since the moth-eye structure is a single layer, it can be easily replicated using ultraviolet nanoimprint lithography (UV-NIL). However the moth-eye structure is fragile, because it has a lot of small sharp needles, and it has been reported that the durability decreases as the aspect ratio increases. The moth-eye structure exhibits anti-reflection function even with a low aspect ratio, but in this study, a fragile moth-eye structure with a high aspect ratio was used to facilitate evaluation of the releasability. In this experiment, a glassy carbon mold with high aspect ratio was release treated using a newly developed release agent. Furthermore, using the "partial-filling method" led to a dramatic improvement in the durability when compared to the conventional method using repeated cycles of the UV-NIL machine.