Duplication of Subcytoband 11E2 of Chromosome 11 Is Regularly Associated with Accelerated Tumor Development in v-abl/myc-Induced Mouse Plasmacytomas

Wiener, Francis; Schmälter, Ann-Kristin; Mowat, Michael; Mai, Sabine

doi:10.1177/1947601910382897

Cited by 7 publications

(12 citation statements)

References 47 publications

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“…The plasmacytoma cell line MOPC460D, a gift of J. Mushinski (National Institutes of Health, Bethesda), were cultured as described (31). T38H mice (32) were obtained from Harwell, UK (33). The mice were kept under specific pathogen-free (SPF) conditions.…”

Section: Mice and B Cell Isolationmentioning

confidence: 99%

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Klewes

Höbsch

Katzir³

et al. 2010

Cytometry Pt A

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Telomeres, the end of chromosomes, are organized in a nonoverlapping fashion and form microterritories in nuclei of normal cells. Previous studies have shown that normal and tumor cell nuclei differ in their 3D telomeric organization. The differences include a change in the spatial organization of the telomeres, in telomere numbers and sizes and in the presence of telomeric aggregates. Previous attempts to identify the above parameters of 3D telomere organization were semi-automated. Here we describe the automation of 3D scanning for telomere signatures in interphase nuclei based on three-dimensional fluorescent in situ hybridization (3D-FISH) and, for the first time, define its sensitivity in tumor cell detection. The data were acquired with a highthroughput scanning/acquisition system that allows to measure cells and acquire 3D images of nuclei at high resolution with 403 or 603 oil and at a speed of 10,000-15,000 cells h 21 , depending on the cell density on the slides. The automated scanning, TeloScan, is suitable for large series of samples and sample sizes. We define the sensitivity of this automation for tumor cell detection. The data output includes 3D telomere positions, numbers of telomeric aggregates, telomere numbers, and telomere signal intensities. We were able to detect one aberrant cell in 1,000 normal cells. In conclusions, we are able to detect tumor cells based on 3D architectural profiles of the genome. This new tool could, in the future, assist in patient diagnosis, in the detection of minimal residual disease, in the analysis of treatment response and in treatment decisions. '

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Section: Mice and B Cell Isolationmentioning

confidence: 99%

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Klewes

Höbsch

Katzir³

et al. 2010

Cytometry Pt A

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“…Two cell types were used, a PreB cell line of BALB/c origin and primary B cells from congenic [T38HxBALB/c]N wild‐type mice . Procedures were conducted according to Animal Protocol 11–019, approved by Central Animal Care Services, University of Manitoba (Winnipeg, MB, Canada).…”

Section: Methodsmentioning

confidence: 99%

Measuring murine chromosome orientation in interphase nuclei

et al. 2015

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The nuclear architecture of a cell may change as a result of various diseases, including cancer. A variety of nuclear features are, therefore, of interest to cell biologists. Recently, several studies have investigated the orientation of chromosomes in the interphase nucleus either visually or semi-automatically. In this article an automated method to measure this orientation is presented. The theoretical difference between performing these measurements in two and three dimensions is discussed and experimentally verified. The results computed from measurements of murine nuclei correspond with results from visual inspection. We found significant differences in the orientation of chromosome 11 between nuclei from a PreB cell line of BALB/c origin and primary B nuclei from congenic [T38HxBALB/c]N wild-type mice. Since our new automatic method concurs with both the visual and semi-automatic methods, we conclude that the automatic method can replace these methods in assessing chromosome orientation. V C 2015 International Society for Advancement of Cytometry

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“…This unique mouse model exhibits a long chromosome T(11;X) and a short chromosome T(X;11). The short chromosome T(X;11) contains cytoband 11E2 and parts of cytoband E1 ( Figure 1 ) [19] . To determine the chromosome orientation in cancer cells and in the same cell lineage, we studied mouse PCT induced in this unique mouse model.…”

Section: Introductionmentioning

confidence: 99%

“…There are slow- and fast-developing PCTs. Slow-onset PCTs are induced only by pristane (2,6,10,14-tetramethylpentadecane) [20] ; fast-onset PCTs are induced by pristane and v-abl/myc [19] , [21] . In the current study, we focused on the fast-onset PCTs.…”

Section: Introductionmentioning

confidence: 99%

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Changes in Nuclear Orientation Patterns of Chromosome 11 during Mouse Plasmacytoma Development

Schmälter

Righolt

Kuzyk

et al. 2015

Translational Oncology

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Studying changes in nuclear architecture is a unique approach toward the understanding of nuclear remodeling during tumor development. One aspect of nuclear architecture is the orientation of chromosomes in the three-dimensional nuclear space. We studied mouse chromosome 11 in lymphocytes of [T38HxBALB/c]N mice with a reciprocal translocation between chromosome X and 11 (T38HT(X;11)) exhibiting a long chromosome T(11;X) and a short chromosome T(X;11) and in fast-onset plasmacytomas (PCTs) induced in the same strain. We determined the three-dimensional orientation of chromosome 11 using a mouse chromosome 11 specific multicolor banding probe. We also examined the nuclear position of the small translocation chromosome T(X;11) which contains cytoband 11E2 and parts of E1. Chromosomes can point either with their centromeric or with their telomeric end toward the nuclear center or periphery, or their position is found in parallel to the nuclear border. In T38HT(X;11) nuclei, the most frequently observed orientation pattern was with both chromosomes 11 in parallel to the nuclear border (“PP”). PCT cells showed nuclei with two or more copies of chromosome 11. In PCTs, the most frequent orientation pattern was with one chromosome in parallel and the other pointing with its centromeric end toward the nuclear periphery (“CP”). There is a significant difference between the orientation patterns observed in T38HT(X;11) and in PCT nuclei (P < .0001).

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Duplication of Subcytoband 11E2 of Chromosome 11 Is Regularly Associated with Accelerated Tumor Development in v-abl/myc-Induced Mouse Plasmacytomas

Cited by 7 publications

References 47 publications

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Measuring murine chromosome orientation in interphase nuclei

Changes in Nuclear Orientation Patterns of Chromosome 11 during Mouse Plasmacytoma Development

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