Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain

Yu, Ermeng; Wang, Wenguang; Yamaji, Naoki; Fukuoka, Shuichi; Che, Jianfei; Ueno, Daisei; Ando, Tsuyu; Deng, Fenglin; Hori, Kiyosumi; Yano, Masahiro; Shen, Ren Fang; Feng, Jian

doi:10.1038/s43016-022-00569-w

Cited by 50 publications

(23 citation statements)

References 45 publications

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“…The accumulation of Cd by plants involves several key physiological processes, including the absorption of external Cd by roots and its subsequent transport from roots to shoots [ 1 ]. These processes need several key members, among which the absorption of external Cd by roots is mainly controlled by the plasma-membrane-localized protein Nramp5 [ 63 , 64 ], while the process of root transport to the shoot mainly depends on the tonoplast-localized P 1B -type heavy metal ATPase HMA3, which reduces the transfer of Cd to the shoots by compartmentalizing it to the vacuoles [ 65 , 66 ]. Gene expression results showed that the expression of ZIP2 in the roots of S. plumbizincicola was inhibited by Cd, but was induced in S. alfredii , and the expression level was higher in S. alfredii than that in S. plumbizincicola .…”

Section: Discussionmentioning

confidence: 99%

Cloning and Functional Characterization of SpZIP2

Han

Tang

Zhang

et al. 2022

Genes

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Zinc (Zn)-regulated and iron (Fe)-regulated transporter-like proteins (ZIP) are key players involved in the accumulation of cadmium (Cd) and Zn in plants. Sedum plumbizincicola X.H. Guo et S.B. Zhou ex L.H. Wu (S. plumbizincicola) is a Crassulaceae Cd/Zn hyperaccumulator found in China, but the role of ZIPs in S. plumbizincicola remains largely unexplored. Here, we identified 12 members of ZIP family genes by transcriptome analysis in S. plumbizincicola and cloned the SpZIP2 gene with functional analysis. The expression of SpZIP2 in roots was higher than that in the shoots, and Cd stress significantly decreased its expression in the roots but increased its expression in leaves. Protein sequence characteristics and structural analysis showed that the content of alanine and leucine residues in the SpZIP2 sequence was higher than other residues, and several serine, threonine and tyrosine sites can be phosphorylated. Transmembrane domain analysis showed that SpZIP2 has the classic eight transmembrane regions. The evolutionary analysis found that SpZIP2 is closely related to OsZIP2, followed by AtZIP11, OsZIP1 and AtZIP2. Sequence alignment showed that most of the conserved sequences among these members were located in the transmembrane regions. A further metal sensitivity assay using yeast mutant Δyap1 showed that the expression of SpZIP2 increased the sensitivity of the transformants to Cd but failed to change the resistance to Zn. The subsequent ion content determination showed that the expression of SpZIP2 increased the accumulation of Cd in yeast. Subcellular localization showed that SpZIP2 was localized to membrane systems, including the plasma membrane and endoplasmic reticulum. The above results indicate that ZIP member SpZIP2 participates in the uptake and accumulation of Cd into cells and might contribute to Cd hyperaccumulation in S. plumbizincicola.

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Section: Discussionmentioning

confidence: 99%

Cloning and Functional Characterization of SpZIP2

Han

Tang

Zhang

et al. 2022

Genes

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“…Paradoxically, overexpression of OsNRAMP5 also markedly decreased Cd accumulation in the shoots and grain of rice by disrupting radial Cd transport into the stele for xylem loading (Chang et al, 2020a). Tandem duplication of an OsNRAMP5 allele in an old rice cultivar, leading to a higher expression level of the gene, was found to decrease Cd accumulation in the grains (Yu et al, 2022). Introgression of this locus into an elite rice cultivar through backcrossing significantly reduced Cd accumulation in the grains without affecting yield (Yu et al, 2022).…”

Section: Strategies To Enhance Tolerance To or Reduce Accumulation Of...mentioning

confidence: 99%

“…Tandem duplication of an OsNRAMP5 allele in an old rice cultivar, leading to a higher expression level of the gene, was found to decrease Cd accumulation in the grains (Yu et al, 2022). Introgression of this locus into an elite rice cultivar through backcrossing significantly reduced Cd accumulation in the grains without affecting yield (Yu et al, 2022).…”

Section: Strategies To Enhance Tolerance To or Reduce Accumulation Of...mentioning

confidence: 99%

Molecular mechanisms underlying the toxicity and detoxification of trace metals and metalloids in plants

Tang

Wang

Chen

et al. 2023

JIPB

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Plants take up a wide range of trace metals/metalloids (hereinafter referred to as trace metals) from the soil, some of which are essential but become toxic at high concentrations (e.g., Cu, Zn, Ni, Co), while others are non-essential and toxic even at relatively low concentrations (e.g., As, Cd, Cr, Pb, and Hg). Soil contamination of trace metals is an increasing problem worldwide due to intensifying human activities. Trace metal contamination can cause toxicity and growth inhibition in plants, as well as accumulation in the edible parts to levels that threatens food safety and human health. Understanding the mechanisms of trace metal toxicity and how plants respond to trace metal stress is important for improving plant growth and food safety in contaminated soils. The accumulation of excess trace metals in plants can cause oxidative stress, genotoxicity, programmed cell death, and disturbance in multiple physiological processes. Plants have evolved various strategies to detoxify trace metals through cell-wall binding, complexation, vacuolar sequestration, efflux, and translocation. Multiple signal transduction pathways and regulatory responses are involved in plants challenged with trace metal stresses. In this review, we discuss the recent progress in understanding the molecular mechanisms involved in trace metal toxicity, detoxification, and regulation, as well as strategies to enhance plant resistance to trace metal stresses and reduce toxic metal accumulation in food crops.

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“…For example, it has been reported that Cd-contaminated soil covered an area of more than 20% of the total farmland area in China ( Tang et al., 2016 ; Xie et al., 2021 ). Cd is one of the most toxic environmental pollutants with a long biological half-life ( Ismael et al., 2019 ; Yu et al., 2022 ), which is concurred to give rise to continuously harmful effects for farmland soil ( Zhang et al., 2021 ). As a result, Cd ion can finally enter into the human body through a contaminated food chain, due to its high mobility from soil to plant and thus causing a Cd accumulation in plant tissues ( Clabeaux et al., 2011 ; Zhu et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Low-level cadmium exposure induced hormesis in peppermint young plant by constantly activating antioxidant activity based on physiological and transcriptomic analyses

et al. 2023

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As one of the most toxic environmental pollutants, cadmium (Cd) has lastingly been considered to have negative influences on plant growth and productivity. Recently, increasing studies have shown that low level of Cd exposure could induce hormetic effect which benefits to plants. However, the underlying mechanisms of Cd-triggered hormesis are poorly understood. In this study, we found that Cd stress treatment showed a hormetic effect on peppermint and Cd treatment with 1.6 mg L-1 concertation manifested best stimulative effects. To explore the hormesis mechanisms of Cd treatment, comparative transcriptome analysis of peppermint young plants under low (1.6 mg L-1) and high (6.5 mg L-1) level of Cd exposure at 0 h, 24 h and 72 h were conducted. Twelve of differentially expressed genes (DEGs) were selected for qRT-PCR validation, and the expression results confirmed the credibility of transcriptome data. KEGG analysis of DEGs showed that the phenylpropanoid biosynthesis and photosynthesis were important under both low and high level of Cd treatments. Interestingly, GO and KEGG analysis of 99 DEGs specifically induced by low level of Cd treatment at 72 h indicated that these DEGs were mainly involved in the pathway of phenylpropanoid biosynthesis and their functions were associated with antioxidant activity. The expression pattern of those genes in the phenylpropanoid biosynthesis pathway and encoding antioxidant enzymes during 72 h of Cd exposure showed that low level of Cd treatment induced a continuation in the upward trend but high level of Cd treatment caused an inverted V-shape. The changes of physiological parameters during Cd exposure were highly consistent with gene expression pattern. These results strongly demonstrate that low level of Cd exposure constantly enhanced antioxidant activity of peppermint to avoid oxidative damages caused by Cd ion, while high level of Cd stress just induced a temporary increase in antioxidant activity which was insufficient to cope with lasting Cd toxicity. Overall, the results presented in this study shed a light on the underlying mechanisms of the Cd-mediated hormesis in plant. Moreover, our study provided a safe method for the efficient utilization of mild Cd-contaminated soil as peppermint is an important cash plant.

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Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain

Cited by 50 publications

References 45 publications

Cloning and Functional Characterization of SpZIP2

Cloning and Functional Characterization of SpZIP2

Molecular mechanisms underlying the toxicity and detoxification of trace metals and metalloids in plants

Low-level cadmium exposure induced hormesis in peppermint young plant by constantly activating antioxidant activity based on physiological and transcriptomic analyses

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