Duplexed aptamer-isothermal amplification-based nucleic acid-templated copper nanoparticles for fluorescent detection of okadaic acid

Shan, Wenchong; Sun, Jingbo; Xu, Wentao; Shao, Bing

doi:10.1016/j.snb.2021.131035

Cited by 18 publications

(9 citation statements)

References 41 publications

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“…This method allows for the oriented immobilization of aptamers, which can offer the advantage of enabling specific modifications to be attached to particular positions within the aptamer sequence. Streptavidin-coated magnetic beads can also be used to connect with aptamers labeled with biotin [ 27 ].…”

Section: Basic Knowledge Of Magnetic Manipulation In Microfluidic Chipmentioning

confidence: 99%

Magnetic Bead Manipulation in Microfluidic Chips for Biological Application

et al. 2023

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Magnetic beads manipulation in microfluidic chips is a promising research field for biological application, especially in the detection of biological targets. In this review, we intend to present a thorough and in-depth overview of recent magnetic beads manipulation in microfluidic chips and its biological application. First, we introduce the mechanism of magnetic manipulation in microfluidic chip, including force analysis, particle properties, and surface modification. Then, we compare some existing methods of magnetic manipulation in microfluidic chip and list their biological application. Besides, the suggestions and outlook for future developments in the magnetic manipulation system are also discussed and summarized.

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Section: Basic Knowledge Of Magnetic Manipulation In Microfluidic Chipmentioning

confidence: 99%

Magnetic Bead Manipulation in Microfluidic Chips for Biological Application

et al. 2023

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“…In order to solve the issue of inferior detection caused by antibodies, aptamers used as sensitive materials have attracted more and more attention. 14,15 Nucleic acid aptamers are short oligonucleotide sequences synthesized in vitro through the systematic evolution of exponential enrichment ligands (SELEX). 16 Owing to the advantages of easy synthesis, easy modication, and temperature and pH stability, aptamers have become a promising effective and signicant sensitive material candidate.…”

Section: Introductionmentioning

confidence: 99%

A 2D carbon nitride-based electrochemical aptasensor with reverse amplification for highly sensitive detection of okadaic acid in shellfish

Chen,

Liu,

Zhu

et al. 2024

Anal. Methods

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“…Fluorescence spectroscopy was a simple and sensitive molecular spectral technique. By combining Apt with fluorescence spectroscopy, some simple, fast, and sensitive Apt fluorescence methods were developed (7)(8)(9)(10)(11). Sun et al (7) developed an upconversing fluorescence DNA probe for the detection of acetamiprid, which consisted of aptamer-conjugated magnetic nanoparticles (Apt-MNPs) and complementary DNA-conjugated upconverting nanoparticles (cDNA-UCNPs).…”

Section: Introductionmentioning

confidence: 99%

“…When the concentration of glypican-3 was from 5.0 to 150.0 ng/ml, the recovery of fluorescence showed a good linear relationship with the concentration of glypican-3, and the detection limit was 2.395 ng/ml. Shan et al ( 9 ) proposed a highly sensitive fluorescence detection method for okadaic acid in seafood, with a LR of 1.0 ng/L to 50.0 μg/L and a detection limit of 1.1 ng/L. Huang et al ( 12 ) proposed a sensor for the detection of Pb 2+ .…”

Section: Introductionmentioning

confidence: 99%

MXene catalytic amplification-fluorescence/absorption dimode aptamer sensor for the detection of trace Pb2+ in milk

Zhi

Wei²,

Zhang³

et al. 2022

Front. Nutr.

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Lead ion (Pb2+) is a toxic heavy metal, which is very harmful to organisms. Therefore, the establishment of a rapid, simple, and sensitive method is of great significance to food safety and human health. It was found that MXeneTi3C2 nanosheet (NS) has a strong catalytic effect on the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) via H2O2 to form the oxidized product (TMBOX); it has a strong fluorescence peak at 415 nm and an absorption (Abs) peak at 295 nm. The aptamer of Pb2+ (Aptpb) can be adsorbed on the surface of an NS to form MXene-Apt conjugates, which reduces its catalytic active sites and inhibits its catalytic activity. When the target Pb2+ is added, it specifically binds with Aptpb to release MXene NSs to enhance the dimode signals. Therefore, a new MXene catalytic fluorescence/absorption dimode aptamer biosenering platform was fabricated for the determination of trace Pb2+ in milk and water samples, with the fluorescence assay linear range (LR) of 5.0 × 10−2-2.0 nmol/L.

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Duplexed aptamer-isothermal amplification-based nucleic acid-templated copper nanoparticles for fluorescent detection of okadaic acid

Cited by 18 publications

References 41 publications

Magnetic Bead Manipulation in Microfluidic Chips for Biological Application

Magnetic Bead Manipulation in Microfluidic Chips for Biological Application

A 2D carbon nitride-based electrochemical aptasensor with reverse amplification for highly sensitive detection of okadaic acid in shellfish

MXene catalytic amplification-fluorescence/absorption dimode aptamer sensor for the detection of trace Pb2+ in milk

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