Duplex real-time PCR assay with high-resolution melt analysis for the detection and quantification of Listeria species and Listeria monocytogenes in meat products

Vishnuraj, M. R.; Ajay, G.; Kumar, Neeraj; Renuka, J.; Niveditha, Pollumahanti; Chauhan, H. Anusha; Vaithiyanathan, S.; Rawool, Deepak B.; Barbuddhe, Sukhadeo B.

doi:10.1007/s13197-023-05695-2

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Moreover, listeriosis has the thirdhighest case mortality rate (up to 30%) among the foodborne diseases [12]. Currently, Listeria contains 27 species [13], but only four of them are commonly isolated from food, namely: L. welshimeri, L. seeligeri, L. monocytogenes, and L. ivanovii [14]. Among them, L. monocytogenes is the only species that is a major human pathogen of public health significance [15,16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Ngema,

Khumalo,

Ojo

et al. 2023

Microorganisms

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The emergence of multidrug-resistant pathogens creates public health challenges, prompting a continuous search for effective novel antimicrobials. This study aimed to isolate marine actinomycetes from South Africa, evaluate their in vitro antimicrobial activity against Listeria monocytogenes and Shiga toxin-producing Escherichia coli, and characterize their mechanisms of action. Marine actinomycetes were isolated and identified by 16S rRNA sequencing. Gas chromatography–mass spectrometry (GC–MS) was used to identify the chemical constituents of bioactive actinomycetes’ secondary metabolites. Antibacterial activity of the secondary metabolites was assessed by the broth microdilution method, and their mode of actions were predicted using computational docking. While five strains showed antibacterial activity during primary screening, only Nocardiopsis dassonvillei strain SOD(B)ST2SA2 exhibited activity during secondary screening for antibacterial activity. GC–MS identified five major bioactive compounds: 1-octadecene, diethyl phthalate, pentadecanoic acid, 6-octadecenoic acid, and trifluoroacetoxy hexadecane. SOD(B)ST2SA2′s extract demonstrated minimum inhibitory concentration and minimum bactericidal concentration, ranging from 0.78–25 mg/mL and 3.13 to > 25 mg/mL, respectively. Diethyl phthalate displayed the lowest bacterial protein-binding energies (kcal/mol): −7.2, dihydrofolate reductase; −6.0, DNA gyrase B; and −5.8, D-alanine:D-alanine ligase. Thus, marine N. dassonvillei SOD(B)ST2SA2 is a potentially good source of antibacterial compounds that can be used to control STEC and Listeria monocytogenes.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Ngema,

Khumalo,

Ojo

et al. 2023

Microorganisms

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show abstract

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Ngema,

Khumalo,

Ojo

et al. 2023

Preprint

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The emergence of multidrug-resistant pathogens such as Listeria monocytogenes and Shiga toxin-producing Escherichia coli (STEC) poses public health challenges, and this has led to a continuous search for effective novel antimicrobial agents. This study aimed to isolate marine actinomycetes from South Africa, evaluate their in vitro antimicrobial activity against Listeria and STEC, and characterize their mechanisms of action. Marine actinoymcetes were isolated and identified by 16S rRNA sequencing. Gas chromatography-mass spectrometry (GC-MS) was used to identify the chemical constituents of bioactive actinomycetes secondary metabolites. Antibacterial activity of the secondary metabolites was assessed by the broth microdilution method and their mode of actions were predicted using computational docking. While five strains showed antibacterial activity during primary screening, only Nocardiopsis dassonvillei strain SOD(B)ST2SA2 exhibited activity during secondary screening for antibacterial activity. GC-MS identified five major bioactive compounds: 1-octadecene, diethyl phthalate, pentadecanoic acid, 6-octadecenoic acid, and tri-fluoroacetoxy hexadecane. SOD(B)ST2SA2`s extract demonstrated the minimum inhibitory con-centration and minimum bactericidal concentration ranging from 0.78–25 mg/ml and 3.13 to > 25 mg/ml, respectively. Diethyl phthalate displayed the lowest bacterial protein binding energies (kcal/mol): −7.2, dihydrofolate reductase; −6.0, DNA gyrase B, and −5.8, D-alanine:D-alanine lig-ase. Thus, marine N. dassonvillei SOD(B)ST2SA2 is a potentially good source of antibacterial compounds that can be used to control STEC and Listeria monocytogenes.

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Hybrid Nucleic Acid Detection Approach for Ion Concentration Polarization Enabled Polymerase Chain Reaction with Reduced Cycle Numbers

Yu,

Li,

Meng

et al. 2024

Preprint

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Duplex real-time PCR assay with high-resolution melt analysis for the detection and quantification of Listeria species and Listeria monocytogenes in meat products

Cited by 3 publications

References 29 publications

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Evaluation of Antimicrobial Activity by Marine <em>Nocardiopsis dassonvillei </em>against Foodborne <em>Listeria monocytogenes</em> and Shiga Toxin-Producing <em>Escherichia coli</em>

Hybrid Nucleic Acid Detection Approach for Ion Concentration Polarization Enabled Polymerase Chain Reaction with Reduced Cycle Numbers

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