DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress

Fortunato, Rodrigo S.; Gomes, Luciana Rodrigues; Munford, Veridiana; Pessôa, Carolina Fittipaldi; Quinet, Annabel; Hecht, Fábio; Kajitani, Gustavo Satoru; Milito, Cristiane; Carvalho, Denise P.; Menck, Carlos Frederico Martins

doi:10.1155/2018/3570526

Cited by 12 publications

(7 citation statements)

References 29 publications

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“…To evaluate the effects of treatment with isoquercitrin on oxidative stress after sciatic nerve crush, qRT-PCR and western blotting are used to examine the NADPH oxidase 4 (Nox4), Duox1, SOD2 and nuclear factor E2-related factor-2 (Nrf2). Nox4 and Duox1 could promote the production of ROS (37,38). Nrf2, a transcription factor, could decrease the production of ROS (39).…”

Section: Isoquercetin Suppresses Oxidative Stress After Sciatic Nerve Crushmentioning

confidence: 99%

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Qiu¹,

Yang

Wang

et al. 2019

Ann. Transl. Med.

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Background: Oxidative stress has been recognized to play a crucial role in the pathogenesis of peripheral nerve injury. Isoquercitrin (quercetin-3-glucoside) is a flavonoid that exhibited many biological activities, including antioxidative effect. However, it is unclear whether isoquercitrin has protective effects on peripheral nerve injury.Methods: Mice treated by isoquercitrin were used as a case group, and mice injected with saline was the control group. Sciatic behavioral function was assessed using SFI and CMAPs were measured by electrophysiology.Schwann cells proliferation and migration were tested using EdU staining and Transwell migration chambers respectively. The expression of oxidative stress related factors were detected by qRT-PCR and Western blotting.Results: In present study, our results demonstrated that isoquercitrin (20 mg/kg/day) treatment achieved significantly higher SFI and higher amplitude of CMAP, promoted the nerve regeneration and remyelination, increased the production of GAP43, NF200, MAG and PMP22, alleviated target muscle atrophy and autophagy, and suppressed the expression of ATG7, PINK1 and Beclin1 in soleus muscles after sciatic nerve crush. In vitro studies found that isoquercitrin promoted the axonal regeneration of DRGs neurons, the proliferation and migration of Schwann cells, and the expression of proliferating cell nuclear antigen (PCNA) in Schwann cells. The administration of isoquercitrin at 40 and 320 μM showed a dose dependent, and high doses of isoquercitrin (160 and 320 μM) showed better performance in promoting axonal regeneration of DRGs neurons, and the proliferation and migration of Schwann cells than low dose of isoquercitrin (40 μM). Furthermore, isoquercitrin significantly inhibited oxidative stress through reducing the production of Nox4 and Duox1, and promoting the expression of Nrf2 and SOD2 in soleus muscles after sciatic nerve crush.Conclusions: Isoquercitrin may promote motor functional recovery and nerve regeneration following peripheral nerve injury though inhibition of oxidative stress, which highlighted the therapeutic values of isoquercitrin as a neuroprotective drug for peripheral nerve repair applications.

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Section: Isoquercetin Suppresses Oxidative Stress After Sciatic Nerve Crushmentioning

confidence: 99%

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Qiu¹,

Yang

Wang

et al. 2019

Ann. Transl. Med.

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“…In addition, there are four unique genes of 209614_at from SIS-SCAD, 206209_s_at, 204271_s_at, 204396_s_at, and 219719_at from SIS-MCP. 219597_s_at (DUOX1) usually is downregulated and associated with lung breast cancer [48,49]. 209555_s_at (CD36) is also related to breast cancer [50] and affects the progression of lung cancer [51].…”

Section: Real Data Analysismentioning

confidence: 99%

Two-Stage Classification with SIS Using a New Filter Ranking Method in High Throughput Data

Kim

2019

Mathematics

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Over the last decade, high dimensional data have been popularly paid attention to in bioinformatics. These data increase the likelihood of detecting the most promising novel information. However, there are limitations of high-performance computing and overfitting issues. To overcome the issues, alternative strategies need to be explored for the detection of true important features. A two-stage approach, filtering and variable selection steps, has been receiving attention. Filtering methods are divided into two categories of individual ranking and feature subset selection methods. Both have issues with the lack of consideration for joint correlation among features and computing time of an NP-hard problem. Therefore, we proposed a new filter ranking method (PF) using the elastic net penalty with sure independence screening (SIS) based on resampling technique to overcome these issues. We demonstrated that SIS-LASSO, SIS-MCP, and SIS-SCAD with the proposed filtering method achieved superior performance of not only accuracy, AUROC, and geometric mean but also true positive detection compared to those with the marginal maximum likelihood ranking method (MMLR) through extensive simulation studies. In addition, we applied it in a real application of colon and lung cancer gene expression data to investigate the classification performance and power of detecting true genes associated with colon and lung cancer.

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“…Moreover, DUOX1-silenced cells continued to proliferate after genotoxic stress. Taken together, these data suggest that DUOX1 is involved in genotoxic stress response in mammary cells, and its downregulation in breast cancer could be related to chemotherapy response (Fortunato et al, 2018).…”

Section: Duoxs and Cancermentioning

confidence: 66%

The role of dual oxidases in physiology and cancer

Faria

Fortunato

2020

Genet. Mol. Biol.

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NOX/DUOX enzymes are transmembrane proteins that carry electrons through biological membranes generating reactive oxygen species. The NOX family is composed of seven members, which are NOX1 to NOX5 and DUOX1 and 2. DUOX enzymes were initially called thyroid oxidases, based on their high expression level in the thyroid tissue. However, DUOX expression has been documented in several extrathyroid tissues, mostly at the apical membrane of the salivary glands, the airways, and the intestinal tract, revealing additional cellular functions associated with DUOX-related H 2 O 2 generation. In this review, we will briefly summarize the current knowledge regarding DUOX structure and physiological functions, as well as their possible role in cancer biology.

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DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress

Cited by 12 publications

References 29 publications

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Two-Stage Classification with SIS Using a New Filter Ranking Method in High Throughput Data

The role of dual oxidases in physiology and cancer

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