Duodenal-Jejunal Bypass Preferentially Elevates Serum Taurine-Conjugated Bile Acids and Alters Gut Microbiota in a Diabetic Rat Model

Zhang, Xiang; Wang, Yanmin; Zhong, Maiying; Li, Teng; Han, Huijian; Zhang, Guangyong; Liu, Shaozhuang; Wang, Meng; Wu, Qunzheng; Hu, Sanyuan

doi:10.1007/s11695-015-2031-x

Cited by 28 publications

(37 citation statements)

References 46 publications

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“…An upregulation of the abundance of Bacteroidetes was observed on animal experiences after RYGB (19,27) and SG (31). However, contrary results were provided on rats after DJB (28). Two studies reported the increase of the phylum Fusobacteria in 2016 (19,22).…”

Section: Microbiota Altered On Strong Evidencementioning

confidence: 96%

“…An increased population of Proteobacteria was observed in diet-induced mice with obesity after RYGB (27). Experimental results on rats showed an increase of Proteobacteria after DJB (28) and RYGB (19,25). In addition, Li et al found substantial shifts of the main gut phyla toward higher concentrations of Proteobacteria, specifically Enterobacter hormaechei (E. hormaechei), from a non-obese rat model characterizing the effect of RYGB (29).…”

Section: Microbiota Altered On Strong Evidencementioning

confidence: 99%

See 1 more Smart Citation

Modulation of the gut microbiome: a systematic review of the effect of bariatric surgery

Guo

Huang

Liu

et al. 2018

European Journal of Endocrinology

152

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Section: Microbiota Altered On Strong Evidencementioning

confidence: 96%

Section: Microbiota Altered On Strong Evidencementioning

confidence: 99%

Modulation of the gut microbiome: a systematic review of the effect of bariatric surgery

Guo

Huang

Liu

et al. 2018

European Journal of Endocrinology

152

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“…Thus, in order to have a better understanding of the relationship between bariatric surgery, gut microbiota, and SCFA, we studied changes in the gut microbiota and SCFA following DJB-sa surgery in Goto-Kakisaki (GK) rats and determined whether these changes were associated with improved glucose metabolism. By applying this model of diabetes, the potential mechanisms by which DJB-sa is beneficial to T2DM patients can be explained from a new perspective (Arora and Bäckhed, 2016;Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Single-Anastomosis Duodenal Jejunal Bypass Improve Glucose Metabolism by Regulating Gut Microbiota and Short-Chain Fatty Acids in Goto-Kakisaki Rats

Zhang

et al. 2020

Front. Microbiol.

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In recent years, bariatric surgery has emerged as a promising treatment for type 2 diabetes. Bariatric surgery is known to cause alterations in the relative abundance and composition of gut microbiota, which may lead to alterations in the levels of Short-Chain Fatty Acids (SCFAs) that are produced during fermentation by gut microbes. However, little is known about the mechanism of improved glucose metabolism mediated by gut microbiota following bariatric surgery. The aim of our study was to explore whether changes in gut microbiota and in fecal SCFA could be detected following singleanastomosis duodenal jejunal bypass (DJB-sa) surgery, a type of bariatric surgery, and whether these alterations might be related to the improvement of glucose metabolism. To this end, we performed DJB-sa or SHAM surgery on Goto-Kakisaki (GK) rats. We then compared the glucose metabolism as well as changes in gut microbiota and SCFAs levels between both groups. Our results showed that DJB-sa surgery was associated with a significant decrease in fasting blood glucose (FBG), intraperitoneal glucose tolerance test (IPGTT), and fasting serum insulin (FSI). And, DJB-sa led to a change in the composition of gut microbiota including an increase in the relative abundance of SCFA-producing bacteria (Bifidobacterium and Subdoligranulum). Moreover, the levels of six SCFAs in feces, as well as the intestinal expression of SCFA receptors including G-protein-coupled receptor 41 (GPR41), G-protein-coupled receptor 43 (GPR43), and G-protein-coupled receptor 109A (GPR109A), and the expression of Glucagon-like peptide-1 (GLP-1) displayed a significant increase following DJB-sa compared with the Sham group. Thus, the gut microbiota may contribute to the improvement of glucose metabolism in type 2 diabetes following DJB-sa. In conclusion, our study shows that DJB-sa improves glucose metabolism by modulating gut microbiota and by increasing short-chain fatty acid production.

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“…Finally, a long-term stability of the changes observed has been recently reported by Tremaroli et al [36]. In addition, in animal models, Zhang et al have recently observed after duodenal-jejunal bypass in a diabetic rat model an increase of Firmicutes and Proteobacteria but a decrease of Bacteroidetes [37].…”

Section: Surgerymentioning

confidence: 54%

Samples and techniques highlighting the links between obesity and microbiota

Angelakis

Lagier

2017

Microbial Pathogenesis

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The composition of gut microbiota and its relationship to human health, particularly its links with obesity remain an ongoing challenge for scientists. The current gold standard for exploring human gut microbiota consists of using stool samples and only applying next generations sequencing techniques, which sometimes generate contradictory results. Here, we comprehensively describe nutrient absorption, fat digestion, carbohydrate and protein absorption, demonstrating that absorption of these diverse nutrients occurs mainly in the stomach and small intestine. Indeed, bariatric surgery, including Roux-en-Y, removes part of the upper intestine, resulting in weight loss, while colonic surgery is associated with a stable weight. However, most studies only use stool samples rather than small intestine samples because of the easy with which this can be accessed. Metagenomics studies are associated with several biases such as extraction and primer biases and depth bias, including the more modern platforms. High-throughput culture-dependent techniques, such as culturomics, which uses rapid identification methods such as MALDI-TOF, remain time-consuming, but have demonstrated their complementarity with molecular techniques. In conclusion, we believe that a comprehensive analysis of the relationships between obesity and gut microbiota requires large-scale studies coupling metagenomics and culture-dependent research, in order to analyse both small intestine and stool samples.

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Duodenal-Jejunal Bypass Preferentially Elevates Serum Taurine-Conjugated Bile Acids and Alters Gut Microbiota in a Diabetic Rat Model

Abstract: DJB preferentially increases serum taurine-conjugated BAs, probably because of more BA reabsorption in the terminal ileum. The gut microbiota is altered with more Firmicutes and Proteobacteria and less Bacteroidetes.

Cited by 28 publications

References 46 publications

Modulation of the gut microbiome: a systematic review of the effect of bariatric surgery

Modulation of the gut microbiome: a systematic review of the effect of bariatric surgery

Single-Anastomosis Duodenal Jejunal Bypass Improve Glucose Metabolism by Regulating Gut Microbiota and Short-Chain Fatty Acids in Goto-Kakisaki Rats

Samples and techniques highlighting the links between obesity and microbiota

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